HBZ (NM_005332) Human Untagged Clone

CAT#: SC122708

HBZ (untagged)-Human hemoglobin, zeta (HBZ)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HBZ
• Synonyms: HBAZ; HBZ-T1; HBZ1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005332 edited
  GGCCCAACTCCAGTGCAGCTGCCCACCCTGCCGCCATGTCTCTGACCAAGACTGAGAGGA
  CCATCATTGTGTCCATGTGGGCCAAGATCTCCACGCAGGCCGACACCATCGGCACCGAGA
  CTCTGGAGAGGCTCTTCCTCAGCCACCCGCAGACCAAGACCTACTTCCCGCACTTCGACC
  TGCACCCGGGGTCCGCGCAGTTGCGCGCGCACGGCTCCAAGGTGGTGGCCGCCGTGGGCG
  ACGCGGTGAAGAGCATCGACGACATCGGCGGCGCCCTGTCCAAGCTGAGCGAGCTGCACG
  CCTACATCCTGCGCGTGGACCCGGTCAACTTCAAGCTCCTGTCCCACTGCCTGCTGGTCA
  CCCTGGCCGCGCGCTTCCCCGCCGACTTCACGGCCGAGGCCCACGCCGCCTGGGACAAGT
  TCCTATCGGTCGTATCCTCTGTCCTGACCGAGAAGTACCGCTGAGCGCCGCCTCCGGGAC
  CCCCAGGACAGGCTGCGGCCCCTCCCCCGTCCTGGAGGTTCCCCAGCCCCACTTACCGCG
  TAATGCGCCAATAAACCAATGAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005332 unedited
  NNGGGGTTTGGATTTGTATACGATCATATAGGCGGCCGCGATTCCCGGGTGGCCCAACTC
  CGTGCAGCTGCCCACCCTGCCGCCTGTCTCTGACCAAGACTGAGAGGACCATCATTGTGT
  CCATGTGGGCCAAGATCTCCACGCAGGCCGACACCATCGGCACCGAGACTCTGGAGAGGC
  TCTTCCTCAGCCACCCGCAGACCAAGACCTACTTCCCGCACTTCGACCTGCACCCGGGGT
  CCGCGCAGTTGCGCGCGCACGGCTCCAAGGTGGTGGCCGCCGTGGGCGACGCGGTGAAGA
  GCATCGACGACATCGGCGGCGCCCTGTCCAAGCTGAGCGAGCTGCACGCCTACATCCTGC
  GCGTGGACCCGGTCAACTTCAAGCTCCTGTCCCACTGCCTGCTGGTCACCCTGGCCGCGC
  GCTTCCCCGCCGACTTCACGGCCGAGGCCCACGCCGCCTGGGACAAGTTCCTATCGGTCG
  TATCCTCTGTCCTGACCGAGAAGTACCGCTGAGCGCCGCCTCCGGGACCCCCAGGACAGG
  CTGCGGCCCCTCCCCCGTCCTGGAGGTTCCCCAGCCCCACTTACCGCGTAATGCGCCAAT
  AAACCAATGAACGNNNAANAAAAANNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAGGGCGGCCGCGNGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGAC
  CCCTCCCCAGTGCCTCTCCTGGCCCTGGAAAGTGCCCTCCAGTGGCCACCAGCCCTGTCC
  TAATAAATNAAGGTGCATCATTTTGTCTGACTGGTGTCCCTCTATAATATAATGGTGGAG
  GGGGTGGGATGGAGCAAGGGGCAAATTGGGAAACAAACCGGAGG
  
• Restriction Sites: Please inquire
• ACCN: NM_005332
• Insert Size: 617 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005332.2, NP_005323.1
• RefSeq Size: 589 bp
• RefSeq ORF: 429 bp
• Locus ID: 3050
• Cytogenetics: 16p13.3
• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS
• Gene Summary: 'Zeta-globin is an alpha-like hemoglobin. The zeta-globin polypeptide is synthesized in the yolk sac of the early embryo, while alpha-globin is produced throughout fetal and adult life. The zeta-globin gene is a member of the human alpha-globin gene cluster that includes five functional genes and two pseudogenes. The order of genes is: 5' - zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 -alpha-1 - theta1 - 3'. [provided by RefSeq, Nov 2009]'