NEDD4 (NM_198400) Human Untagged Clone

CAT#: SC123396

NEDD4 (untagged)-Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEDD4
• Synonyms: NEDD4-1; RPF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_198400 edited
  ATGGCACAAAGCTTACGATTGCACTTTGCAGCCAGAAGAAGCAATACTTACCCTTTGTCA
  GAAACCTCCGGAGATGACTTGGATAGCCATGTTCACATGTGCTTCAAAAGACCAACACGG
  ATTTCAACGTCTAACGTTGTTCAAATGAAGCTGACTCCCAGACAGACTGCACTAGCTCCG
  TTAATAAAGGAAAACGTTCAGTCTCAAGAAAGATCATCTGTTCCCTCATCTGAAAATGTT
  AATAAAAAGAGCAGCTGTCTACAGATTTCACTACAGCCAACAAGGTACAGTGGATATCTT
  CAGTCTAGCAATGTCTTAGCTGATAGTGATGATGCTTCGTTTACTTGTATCTTGAAGGAT
  GGTATTTACAGTAGTGCTGTGGTCGATAATGAATTGAATGCTGTGAATGATGGTCACCTT
  GTAAGCAGTCCAGCCATTTGTAGTGGTAGCCTTAGTAACTTTTCAACCAGTGATAATGGG
  TCTTACAGCAGCAACGGTAGTGATTTTGGGTCATGTGCAAGTATCACAAGTGGAGGTTCA
  TATACTAACAGTGTCATCAGTGACAGTAGTAGTTATACTTTTCCACCAAGTGATGATACT
  TTTTTGGGTGGAAACTTACCTTCTGACAGCACCTCCAATAGAAGTGTGCCAAACAGGAAT
  ACTACTCCTTGTGAAATTTTTTCAAGAAGTACAAGTACAGATCCTTTTGTCCAGGATGAC
  TTGGAACATGGATTAGAGATTATGAAATTGCCAGTGAGCAGGAACACAAAAATTCCACTA
  AAACGTTACTCCTCCTTAGTCATTTTTCCTAGGAGTCCTTCAACTACCCGACCGACTTCT
  CCAACAAGTCTGTGTACTCTTCTGAGCAAAGGATCCTATCAAACTTCACACCAGTTTATT
  ATTTCTCCTAGTGAAATTGCACATAATGAGGATGGCACTAGTGCTAAAGGATTTCTTTCA
  ACAGCTGTCAATGGACTTCGGTTATCTAAAACAATTTGTACCCCCGGAGAAGTAAGAGAC
  ATACGGCCGCTTCACAGGAAGGGCTCGTTACAGAAGAAAATTGTTCTTTCGAATAATACT
  CCCAGACAGACTGTCTGTGAAAAGTCATCTGAAGGATATTCTTGTGTTTCAGTGCATTTC
  ACCCAACGAAAAGCAGCTACATTAGACTGTGAAACAACAAATGGTGATTGTAAACCAGAA
  ATGTCAGAAATTAAGCTTAATTCTGATTCAGAGTATATTAAGCTCATGCATAGGACATCT
  GCATGTTTGCCATCCTCCCAAAATGTAGATTGTCAAATAAATATCAATGGAGAATTGGAA
  AGACCACATTCACAGATGAACAAAAACCATGGTATTTTACGAAGAAGTATTTCATTGGGA
  GGAGCTTATCCAAATATTTCCTGTCTATCCAGCCTTAAGCACAATTGTTCTAAAGGGGGA
  CCATCTCAGTTACTCATAAAGTTTGCATCTGGAAATGAAGGTAAAGTGGATAATTTATCA
  AGAGACAGCAACAGAGATTGCACAAATGAACTGTCTAATTCTTGCAAGCCTGGCTGGGTT
  GTTTTGGACCAACCAGATGCTGCTTGCCATTTGCAGCAACAACAAGAACCTTCTCCTCTA
  CCTCCAGGGTGGGAAGAGAGGCAGGATATCCTTGGAAGGACCTATTATGTAAACCATGAA
  TCTAGAAGAACACAGTGGAAAAGACCAACCCCTCAGGACAACCTAACAGATGCTGAGAAT
  GGCAACATTCAACTGCAAGCACAACGTGCATTTACCACCAGGCGGCAGATATCCGAGGAA
  ACAGAAAGTGTTGACAACCGAGAGTCTTCCGAGAACTGGGAAATTATAAGAGAAGATGAA
  GCCACCATGTATAGCAACCAGGCCTTCCCATCACCTCCACCGTCAAGTAACTTGGATGTT
  CCAACTCATCTTGCAGAAGAATTGAATGCCAGACTCACCATTTTTGGAAATTCAGCCGTG
  AGCCAGCCAGCATCGAGCTCAAATCATTCCAGCAGAAGAGGCAGCTTACAAGCCTATACT
  TTTGAGGAACAACCTACACTTCCTGTGCTTTTGCCTACTTCATCTGGATTACCACCAGGT
  TGGGAAGAAAAACAAGATGAAAGAGGAAGATCATATTATGTAGATCACAATTCCAGAACG
  ACTACTTGGACAAAGCCCACTGTACAGGCCACAGTGGAGACCAGTCAGCTGACCTCAAGC
  CAGAGTTCTGCAGGCCCTCAATCACAAGCCTCCACCAGTGATTCAGGCCAGCAGGTGACC
  CAGCCATCTGAAATTGAGCAAGGATTCCTTCCTAAAGGCTGGGAAGTCCGGCATGCACCA
  AATGGGAGGCCTTTCTTTATTGACCACAACACTAAAACCACCACCTGGGAAGATCCAAGA
  TTGAAAATTCCAGCCCATCTGAGAGGAAAGACATCACTTGATACTTCCAATGATCTAGGG
  CCTTTACCTCCAGGATGGGAAGAGAGAACTCACACAGATGGAAGAATCTTCTACATAAAT
  CACAATATAAAAAGAACACAATGGGAAGATCCTCGGTTGGAGAATGTAGCAATAACTGGA
  CCAGCAGTGCCCTACTCCAGGGATTACAAAAGAAAGTATGAGTTCTTCCGAAGAAAGTTG
  AAGAAGCAGAATGACATTCCAAACAAATTTGAAATGAAACTTCGCCGAGCAACTGTTCTT
  GAAGACTCTTACCGGAGAATTATGGGTGTCAAGAGAGCAGACTTCCTGAAGGCTCGACTG
  TGGATTGAGTTTGATGGTGAAAAGGGATTGGATTATGGAGGAGTTGCCAGAGAATGGTTC
  TTCCTGATCTCAAAGGAAATGTTTAACCCTTATTATGGGTTGTTTGAATATTCTGCTACG
  GACAATTATACCCTACAGATAAATCCAAACTCTGGATTGTGTAACGAAGATCACCTCTCT
  TACTTCAAGTTTATTGGTCGGGTAGCTGGAATGGCAGTTTATCATGGCAAACTGTTGGAT
  GGTTTTTTCATCCGCCCATTTTACAAGATGATGCTTCACAAACCAATAACCCTTCATGAT
  ATGGAATCTGTGGATAGTGAATATTACAATTCCCTAAGATGGATTCTTGAAAATGACCCA
  ACAGAATTGGACCTCAGGTTTATCATAGATGAAGAACTTTTTGGACAGACACATCAACAT
  GAGCTGAAAAATGGTGGATCAGAAATAGTTGTCACCAATAAGAACAAAAAGGAATATATT
  TATCTTGTAATACAATGGCGATTTGTAAACCGAATCCAGAAGCAAATGGCTGCTTTTAAA
  GAGGGATTCTTTGAACTAATACCACAGGATCTCATCAAAATTTTTGATGAAAATGAACTA
  GAGCTTCTTATGTGTGGACTGGGAGATGTTGATGTGAATGACTGGAGGGAACATACAAAG
  TATAAAAATGGCTACAGTGCAAATCATCAGGTTATACAGTGGTTTTGGAAGGCTGTTTTA
  ATGATGGATTCAGAAAAAAGAATAAGATTACTTCAGTTTGTCACTGGCACATCTCGGGTG
  CCTATGAATGGATTTGCTGAACTATACGGTTCAAATGGACCACAGTCATTTACAGTTGAA
  CAGTGGGGTACTCCTGAAAAGCTGCCAAGAGCTCATACCTGTTTTAATCGCCTGGACTTG
  CCACCTTATGAATCATTTGAAGAATTATGGGATAAACTTCAGATGGCAATTGAAAACACC
  CAGGGCTTTGATGGAGTTGATTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_198400
• Insert Size: 5500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF was fully sequenced and matches to NM_198400.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198400.1, NP_940682.1
• RefSeq Size: 7084 bp
• RefSeq ORF: 3744 bp
• Locus ID: 4734
• Cytogenetics: 15q21.3
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Ubiquitin mediated proteolysis
• Gene Summary: 'This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. [provided by RefSeq, Jul 2016]'
  Transcript Variant: This variant (2) encodes isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.