RASGRF1 (BC040275) Human Untagged Clone

CAT#: SC123451

RASGRF1 (untagged)-Human Ras protein-specific guanine nucleotide-releasing factor 1 (cDNA clone MGC:35460 IMAGE:5192914), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RASGRF1
• Synonyms: CDC25; CDC25L; GNRP; GRF1; GRF55; guanine nucleotide-releasing factor, 55 kD; guanine nucleotide exchange factor; H-GRF55; PP13187; Ras-specific guanine nucleotide-releasing factor, CDC25 homolog; Ras-specific nucleotide exchange factor CDC25; Ras protein
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC040275 edited
  CGGCACCGGAGGAGGGTCGGGCGGCATCTTCCGGGTACTGGGGCTCTGCGGAGCGGAGAA
  GAGGTTCCAGCGGGGAATGGTATATCTGGATTCAAGAAGCCGCGGCTCGGCGCCAGATCC
  TGGAGTGTAGATATTTGGGGGGAGGGGAGAGCTAGAGGGAGCGAGCGAGCGAGCGCCAGA
  GAGAGGCAGCGCGCAGCGCGCACGGACGGGGGGCTGCTGCGCGGAGAAGATGTAAGCGCG
  TGGGGTCTCGCTGGCCTCTGAGCACCATGCAGAAGGGGATCCGGCTGAATGATGGCCACG
  TCGCGTCCCTGGGACTGCTGGCGCGCAAGGACGGCACGCGCAAAGGCTACCTGAGCAAGC
  GGAGTTCGGACAACACAAAATGGCAAACCAAGTGGTTCGCGCTGCTGCAGAACCTGCTCT
  TCTACTTCGAGAGCGACTCGAGCTCGCGGCCCTCGGGGCTTTACCTGCTGGAGGGCTGCG
  TCTGCGACCGCGCGCCCTCCCCCAAGCCGGCGCTGTCGGCCAAGGAGCCGCTGGAGAAAC
  AGCATTACTTCACGGTGAACTTCAGCCATGAGAACCAGAAAGCCTTGGAGCTGAGGACAG
  AGGACGCAAAAGATTGTGACGAATGGGTGGCAGCCATTGCACATGCCAGCTACAGGACCC
  TCGCCACAGAGCATGAGGCATTAATGCAGAAATACCTGCACCTGCTGCAGATCGTGGAGA
  CAGAGAAGACCGTGGCCAAGCAGCTTCGGCAGCAGATCGAGGATGGGGAGATCGAGATCG
  AGCGGCTGAAGGCAGAGATCACATCCCTGCTCAAGGACAATGAGCGCATCCAGTCCACCC
  AGACTGTCGCCCCCAACGATGAAGACAGCGACATCAAGAAAATTAAGAAGGTGCAGAGCT
  TCCTGCGGGGCTGGCTGTGCCGGCGGAAGTGGAAGACCATCATCCAGGACTACATCCGGT
  CACCCCATGCTGACAGCATGCGCAAGAGGAACCAGGTGGTGTTCAGCATGCTGGAGGCTG
  AGGCTGAGTACGTGCAGCAGCTGCACATCCTTGTCAACAATTTCCTGCGCCCGCTGCGGA
  TGGCCGCCAGCTCCAAGAAGCCTCCCATCACACACGACGACGTCAGCAGCATCTTCCTGA
  ACAGCGAAACCATCATGTTTTTACATCAGATCTTTTACCAAGGCCTGAAGGCCCGCATCT
  CCAGCTGGCCCACGCTGGTCCTGGCTGACCTATTTGACATCCTGCTGCCCATGCTCAACA
  TCTACCAAGAGTTCGTCCGCAACCACCAGTACAGCCTGCAGATCCTGGCCCACTGCAAGC
  AGAACCGTGACTTCGACAAGCTGCTGAAGCACTACGAGGCCAAGCCTGACTGCGAGGAGA
  GGACGCTGGAGACCTTCCTCACCTACCCCATGTTCCAGATCCCCAGGTACATCCTGACCC
  TCCATGAGCTCCTGGCCCACACGCCTCATGAGCACGTTGAGCGCAACAGCCTGGACTACG
  CCAAGTCCAAACTGGAGGAGCTGTCCAGAATAATGCACGATGAAGTAAGTGAGACGGAGA
  ACATCCGGAAAAACCTGGCCATCGAGCGCATGATCATCGAAGGCTGTGAGATCCTCCTGG
  ACACCAGCCAGACCTTTGTGAGACAAGGTTCCCTCATTCAGGTGCCCATGTCTGAAAAGG
  GCAAGATCACCAGGGGGCGCCTGGGGTCTCTCTCCCTAAAGAAAGAGGGCGAGCGACAGT
  GCTTCCTGTTTTCTAAGCATCTGATTATCTGTACCAGAGGCTCTGGAGGGAAGCTTCACT
  TGACCAAGAATGGAGTCATATCCCTCATTGACTGCACTTTATTGGAGGAGCCAGAAAGCA
  CGGAGGAGGAAGCCAAAGGATCCGGCCAAGACATAGATCACTTGGATTTTAAAATCGGGG
  TGGAGCCAAAGGATTCCCCGCCCTTTACAGTCATCCTAGTGGCCTCGTCCAGACAGGAGA
  AGGCAGCGTGGACCAGTGACATCAGCCAGTGTGTGGATAACATCCGATGCAATGGGCTCA
  TGATGAACGCATTTGAAGAAAATTCCAAGGTCACTGTGCCGCAGATGATCAAGTCCGACG
  CCTCCTTATATTGTGATGATGTTGACATTCGCTTCAGCAAAACCATGAACTCCTGCAAAG
  TGCTGCAGATCCGCTACGCCAGTGTGGAGCGGCTGCTGGAGAGGCTGACGGACCTGCGCT
  TCCTGAGCATCGACTTCCTCAACACCTTCCTGCACTCCTACCGCGTCTTCACCACCGCCA
  TCGTGGTCCTGGACAAGCTCATTACCATCTACAAGAAGCCTATCAGTGCCATTCCTGCCA
  GGTCGCTGGAGCTCCTGTTTGCCAGTGGCCAGAACAATAAGCTCCTGTACGGTGAACCCC
  CCAAGTCCCCGCGCGCCACCCGCAAGTTCTCCTCGCCGCCACCTCTGTCCATCACCAAGA
  CATCGTCACCGAGCCGCCGGCGGAAGCTCTCCCTGAACATCCCCATCATCACTGGCGGCA
  AGGCCCTGGACCTGGCCGCCCTCAGCTGCAACTCCAATGGCTACACCAGCATGTACTCGG
  CCATGTCACCCTTCAGCAAGGCCACGCTGGACACCAGCAAGCTCTATGTGTCCAGCAGCT
  TCACCAACAAGATTCCAGATGAGGGCGATACGACCCCTGAGAAGCCCGAAGACCCTTCAG
  CGCTCAGCAAGCAGAGCTCAGAAGTCTCCATGAGAGAGGAGTCAGATATTGATCAAAACC
  AGAGTGATGATGGTGATACTGAAACATCACCAACTAAATCTCCAACAACACCCAAATCAG
  TCAAAAACAAAAATTCTTCAGAGTTCCCACTCTTTTCCTATAACAATGGAGTCGTCATGA
  CCTCCTGTCGTGAACTGGACAATAACCGCAGTGCCTTGTCGGCCGCCTCTGCCTTTGCCA
  TAGCAACCGCCGGGGCCAACGAGGGCACCCCAAACAAGGAGAAGTACCGGAGGATGTCCT
  TAGCCAGTGCAGGGTTTCCCCCAGACCAGAGGAATGGAGACAAGGAGTTTGTGATCCGCA
  GAGCAGCCACCAATCGTGTCTTGAACGTGCTCCGCCACTGGGTGTCCAAGCACTCTCAGG
  ACTTTGAGACCAACGATGAGCTCAAATGCAAGGTGATCGGCTTCCTGGAAGAAGTCATGC
  ACGACCCGGAGCTCCTGACCCAGGAGCGGAAGGCTGCAGCCAACATCATCAGGACTCTGA
  CCCAGGAGGACCCAGGTGACAACCAGATCACGCTGGAGGAGATCACGCAGATGGCTGAAG
  GCGTGAAGGCTGAGCCCTTTGAAAACCACTCAGCCCTGGAGATCGCGGAGCAGCTGACCC
  TGCTAGATCACCTCGTCTTCAAGAAGATTCCTTATGAGGAGTTCTTCGGACAAGGATGGA
  TGAAACTGGAAAAGAATGAAAGGACCCCTTATATCATGAAAACCACTAAGCACTTCAATG
  ACATCAGTAACTTGATTGCTTCAGAAATCATCCGCAACGAGGACATCAACGCCAGGGTGA
  GCGCCATCGAGAAGTGGGTGGCCGTAGCTGACATATGCCGCTGCCTCCACAACTACAATG
  CCGTACTGGAGATCACCTCGTCCATGAACCGCAGTGCAATCTTCCGGCTCAAAAAGACGT
  GGCTCAAAGTCTCTAAGCAGGTGCGGGCAGGAGGCTGGCACCACTGCCTCCCTGGGGCTG
  GATTGGGGCTGGGGCAGGAAGACCCGTGGGCTGGCCACTGGGCATCCACAAGTGGGCAAA
  GGACTGGGCTGAGGTCTAACACTCCCAGGTAAAACAGAATAGCTTCCCCTATATCACCTG
  GGACCAGATAGCAGGCCCACAGATATGGCAGTGAGACCGTGGACTGTAATCCCCACTTGA
  GTAATTAATACCTCAAAGTGGAAAGAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC040275 unedited
  GAGTATAGAGGGNGNAAACCACTCATTATAGGCGGCCGACGATTCCCGGGATCGGCACCG
  GNAGAGGGTCGGGCGGCATCTTCCGGGTACTGGGGCTCTGCGGAGCGGAGAAGAGGTTCC
  AGCGGGGAATGGTATATCTGGATTCAAGAAGCCGCGGCTCGGCGCCAGATCCTGGAGTGT
  AGATATTTGGGGGGAGGGGAGAGCTAGAGGGAGCGAGCGAGCGAGCGCCAGAGAGAGGCA
  GCGCGCAGCGCGCACGGACGGGGGGCTGCTGCGCGGAGAAGATGTAAGCGCGTGGGGTCT
  CGCTGGCCTCTGAGCACCATGCAGAAGGGGATCCGGCTGAATGATGGCCACGTCGCGTCC
  CTGGGACTGCTGGCGCGCAAGGACGGCACGCGCAAAGGCTACCTGAGCAAGCGGAGTTCG
  GACAACACAAAATGGCAAACCAAGTGGTTCGCGCTGCTGCAGAACCTGCTCTTCTACTTC
  GAGAGCGACTCGAGCTCGCGGCCCTCGGGGCTTTACCTGCTGGAGGGCTGCGTCTGCGAC
  CGCGCGCCCTCCCCCAAGCCGGCGCTGTCGGCCAAGGAGCCGCTGGAGAAACAGCATTAC
  TTCACGGTGAACTTCAGCCATGAGAACCAGANAGCCTTGGAGCTGAGGACAGAGGACGCA
  AAAGATTGTGACGAATGGGTGGCAGCCATTGCACATGCCAGCTACAGGACCCTCGCCACA
  GAGCATGAGGCATTAATGCAGAAATACCTGCACCTGCTGCAGATCGTGGAGACAGAGAAG
  ACCGTGGCCAAGCAGCTTCGGCAGCAGATCGAGGATGGGGAGATCGAGATCCAGCGGCTG
  AAGGCAGAGATCACATCCCTGCTCAAGGACAATGAGCGCATCCAGTCCACCCAGATGTCG
  NCCCCCCCAATGAAGACAGA
  
• Restriction Sites: Please inquire
• ACCN: BC040275
• Insert Size: 3950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC040275.1, AAH40275.1
• RefSeq Size: 3950 bp
• Locus ID: 5923
• Cytogenetics: 15q25.1
• Protein Families: Druggable Genome
• Protein Pathways: Focal adhesion, MAPK signaling pathway
• Gene Summary: 'The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Mar 2009]'