DGKG (NM_001346) Human Untagged Clone

CAT#: SC124112

DGKG (untagged)-Human diacylglycerol kinase, gamma 90kDa (DGKG), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DGKG
• Synonyms: DAGK3; DGK-GAMMA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001346, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGTGAAGAACGGTGGGTCTCCCTCACTCCAGAAGAATTTGACCAACTCCAGAAATATTCAGAATATT
  CCTCCAAGAAGATAAAAGATGCCTTGACTGAATTTAATGAGGGTGGGAGCCTCAAACAATATGACCCACA
  TGAGCCGATTAGCTATGATGTCTTCAAGCTGTTCATGAGGGCGTACCTGGAGGTGGACCTTCCCCAGCCA
  CTGAGCACTCACCTCTTCCTGGCCTTCAGCCAGAAGCCCAGACACGAGACCTCTGACCACCCGACGGAGG
  GAGCCAGCAACAGTGAGGCCAACAGCGCAGATACTAATATACAGAATGCAGATAATGCCACCAAAGCAGA
  CGAGGCCTGTGCCCCTGATACTGAATCAAATATGGCTGAGAAGCAAGCACCAGCTGAAGACCAAGTGGCT
  GCGACCCCCCTGGAACCCCCCGTCCCTCGGTCTTCAAGCTCGGAATCCCCAGTGGTATACCTGAAGGATG
  TTGTGTGCTACCTGTCCCTGCTGGAGACGGGGAGGCCTCAGGATAAGCTGGAGTTCATGTTTCGCCTCTA
  TGATTCAGATGAGAACGGTCTCCTGGACCAAGCGGAGATGGATTGCATTGTCAACCAAATGCTGCATATT
  GCCCAGTACCTGGAGTGGGATCCCACAGAGCTGAGGCCTATATTGAAGGAGATGCTGCAAGGGATGGACT
  ACGACCGGGACGGCTTTGTGTCTCTACAGGAATGGGTCCATGGAGGGATGACCACCATCCCATTGCTGGT
  CCTCCTGGGGATGGATGACTCTGGCTCCAAGGGGGATGGGCGGCACGCCTGGACCATGAAGCACTTCAAG
  AAACCAACCTACTGCAACTTCTGCCATATCATGCTCATGGGCGTCCGCAAGCAAGGCCTGTGCTGCACTT
  ACTGTAAATACACTGTCCACGAACGCTGTGTGTCCAGAAACATTCCTGGTTGTGTCAAAACGTACTCAAA
  AGCCAAAAGGAGTGGTGAGGTGATGCAGCACGCATGGGTGGAAGGGAACTCCTCCGTCAAGTGTGACCGG
  TGCCACAAAAGTATCAAGTGCTACCAGAGTGTCACCGCGCGGCACTGCGTGTGGTGCCGGATGACGTTTC
  ACCGCAAATGTGAATTATCAACGTTGTGTGACGGTGGGGAACTCAGAGACCACATCTTACTGCCCACCTC
  CATATGCCCCATCACCCGGGACAGGCCAGGTGAGAAGTCTGATGGCTGCGTGTCCGCCAAGGGCGAACTT
  GTCATGCAGTATAAGATCATCCCCACCCCGGGTACCCACCCCCTGCTGGTCTTGGTGAACCCCAAGAGTG
  GAGGGAGACAAGGAGAAAGAATTCTTCGGAAATTCCACTATCTGCTCAACCCCAAACAAGTTTTCAACCT
  GGACAATGGGGGGCCTACTCCAGGGTTGAACTTTTTCCGTGATACTCCAGACTTCCGTGTTTTGGCCTGT
  GGTGGAGATGGGACAGTTGGCTGGATTTTGGATTGCATTGATAAGGCCAACTTTGCAAAGCATCCACCAG
  TGGCTGTCCTGCCTCTTGGAACAGGAAATGACCTTGCCCGTTGTCTCCGCTGGGGAGGAGGTTATGAAGG
  GGGCAGCTTGACAAAAATCCTGAAAGACATTGAGCAGAGCCCCTTGGTGATGCTGGACCGCTGGCATCTG
  GAAGTCATCCCCAGAGAGGAAGTGGAAAACGGGGACCAGGTCCCATACAGCATCATGAACAACTATTTCT
  CCATTGGTGTGGACGCTTCCATTGCACACAGATTCCATGTGATGAGAGAGAAACATCCTGAAAAATTCAA
  CAGCAGGATGAAGAACAAGCTGTGGTACTTTGAATTTGGCACCTCGGAGACTTTTGCAGCGACCTGCAAG
  AAACTCCACGACCACATTGAGTTGGAGTGTGATGGGGTTGGGGTGGACCTGAGCAACATCTTCCTGGAAG
  GCATTGCCATTCTCAACATTCCCAGCATGTACGGAGGCACCAATCTCTGGGGAGAAAACAAGAAGAACCG
  GGCTGTGATCCGGGAAAGCAGGAAGGGTGTCACTGACCCCAAAGAACTGAAATTCTGCGTTCAAGACCTC
  AGTGACCAGCTCCTTGAAGTGGTGGGGCTAGAAGGAGCCATGGAGATGGGGCAGATCTACACCGGCCTGA
  AGAGTGCAGGCAGGAGGCTGGCCCAGTGCGCCTCTGTCACCATCAGGACAAACAAGCTGCTGCCAATGCA
  AGTGGATGGAGAACCCTGGATGCAGCCATGTTGCACGATTAAAATTACTCACAAGAACCAAGCGCCCATG
  ATGATGGGGCCTCCCCAGAAGAGCAGCTTCTTCTCGTTGAGAAGGAAGAGCCGTTCAAAAGACTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001346 unedited
  NTGTCAAAATTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGGGCCAC
  GGAGATAGACAGCTTTGGAGCTGCTGAACTCCGAGCACAGGGTGAAGACCCCGGCGCTAC
  CAACCACAGCCTGGCAGCCTGGTCTCCGCGGCACCCACTGGGGCTGCATCCCCCTCCCCC
  GAGAGGGCTGCGCAGGCGGGAAGACGCCCAGAGGCCAGCTTCGGTCCCCCTTCTGTCTCT
  CGGTTCCTCTTTCCTCCCAAGTAAGGGAATAAACCGCGAAGAAGGAGCGCCCCGGGCCAC
  CGCGCAACCAAGTGTTGCCTGGTGAGGAAGAGCCAGGACTTCTGAATTTACCTTGAATAC
  AGACAGGAGGATGTTGCCTAAGGAATAGCAGAGATCTTGTCTCATCTTCTGAGAGGTGCC
  TGCTGCTGCTGTATACACTTGAGTGCTCCCAGAAGTCTCCTGAAAGGCTTACATCGCAAA
  CCTGCAATGAGCCAGGCCCTGGGCTGGGCCTCCACTTCAGCCTAGTGAACAAAACTCCAT
  CACTGCCCTTTAGCCACTCACATAAAGTTTAAAAATGGGTGAAGAACGGTGGGTCTCCCT
  CACTCCAGAAGAATTTGACCAACTCCAGAAATATTCAGAATATTCCTCCAAGAAGATAAA
  AGATGCCTTGACTGAATTTAATGAGGGTGGGAGCCTCANACAATATGACCCACATGAGCC
  GATTAGCTATGATGTCTTCAAGCTGTTCATGAGGGCGTACCTGGAGGTGGACCTTCCCCA
  GCCACTGAGCACTCACCTCTTCCTGGCCTTCAGCCAGAAGCCCAGACACGAGACCTCTGA
  CCACCCGACGGAGGGNAGCCAGCACAGTGAGGCCAACAGCGCAGATACTAATATACAGAA
  TGCAGATNATGCCACCAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001346 unedited
  AGGGGNNAGGTNNNNTTTGCCTGCATGCAACTTCCAGGCCAGGAAAGGCACTGGGGAGGG
  GTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTA
  GAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACACAATA
  TTATGACACCAATTTGAAAAATAAATCCCAGTTTCTCCGCTCTCCCTCCCAGGTGTTCAC
  AGAACTAAGAAAATAAATCCCACATCTAAGGTGTGCTATGCAGGCAGCTCTGGGGGCTCT
  TGGAGCCGCCTCTAAGCAAACATGTAGACCCCAGGAGAAGGTTCCTGGAAAATAACAAGT
  GCCCACCGACGGCGCAGAAACCCAAGAATGGAAATACAGCTTTCCACGGCCTTTCTGCCT
  CTTCAGCAGGTTCACGGAGAAGTTGTCACTCAAAGGACGACCAAGAAACCAACGTGCGCC
  TGCTGAGCCCTGCCAAGGATTATGCTCTGAGAGCCAAAAGCCGTTTTGTCTCTGTACAGA
  GGGAACTTTGTGCAAATTCTCTGGAACCAGAGCTTCTTCCCTTGGCCACATTTGATCTCA
  CCATAGGCAGGAAACCTTGAGCCTTGTTCTCGGGAGCGTCCATGAAGAAACTTTGAGGGG
  CTGTAGAAAGAAGCGGGATTTCCCTCATCTAAAAAATTCTGCTAAAGCTGTGGGAAATTG
  TGAGTCTCTANNCANCCCACCCTTTGCCCTAGTCATTCAAGTGTTGTGCACTGCTTTTAA
  GTTACCT
  
• Restriction Sites: Please inquire
• ACCN: NM_001346
• Insert Size: 3900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001346.1, NP_001337.1
• RefSeq Size: 3758 bp
• RefSeq ORF: 2376 bp
• Locus ID: 1608
• Cytogenetics: 3q27.2-q27.3
• Domains: DAGKa, DAGKc, EFh, DAG_PE-bind
• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: 'This gene encodes an enzyme that is a member of the type I subfamily of diacylglycerol kinases, which are involved in lipid metabolism. These enzymes generate phosphatidic acid by catalyzing the phosphorylation of diacylglycerol, a fundamental lipid second messenger that activates numerous proteins, including protein kinase C isoforms, Ras guanyl nucleotide-releasing proteins and some transient receptor potential channels. Diacylglycerol kinase gamma has been implicated in cell cycle regulation and in the negative regulation of macrophage differentiation in leukemia cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).