Angiotensin II Type 1 Receptor (AGTR1) (NM_031850) Human Untagged Clone

CAT#: SC124238

AGTR1 (untagged)-Human angiotensin II receptor, type 1 (AGTR1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AGTR1
• Synonyms: AG2S; AGTR1B; AT1; AT1AR; AT1B; AT1BR; AT1R; AT2R1; HAT1R
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_031850, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGAAAATGAATCACAAGTCAACTGACAGTCCAAAGGCTCCACAGCTCAGAGGAGGTGTATTTGATA
  TAGTGTTTGCAACAAATTCGACCCAGGTGATCAAAATGATTCTCAACTCTTCTACTGAAGATGGTATTAA
  AAGAATCCAAGATGATTGTCCCAAAGCTGGAAGGCATAATTACATATTTGTCATGATTCCTACTTTATAC
  AGTATCATCTTTGTGGTGGGAATATTTGGAAACAGCTTGGTGGTGATAGTCATTTACTTTTATATGAAGC
  TGAAGACTGTGGCCAGTGTTTTTCTTTTGAATTTAGCACTGGCTGACTTATGCTTTTTACTGACTTTGCC
  ACTATGGGCTGTCTACACAGCTATGGAATACCGCTGGCCCTTTGGCAATTACCTATGTAAGATTGCTTCA
  GCCAGCGTCAGTTTCAACCTGTACGCTAGTGTGTTTCTACTCACGTGTCTCAGCATTGATCGATACCTGG
  CTATTGTTCACCCAATGAAGTCCCGCCTTCGACGCACAATGCTTGTAGCCAAAGTCACCTGCATCATCAT
  TTGGCTGCTGGCAGGCTTGGCCAGTTTGCCAGCTATAATCCATCGAAATGTATTTTTCATTGAGAACACC
  AATATTACAGTTTGTGCTTTCCATTATGAGTCCCAAAATTCAACCCTCCCGATAGGGCTGGGCCTGACCA
  AAAATATACTGGGTTTCCTGTTTCCTTTTCTGATCATTCTTACAAGTTATACTCTTATTTGGAAGGCCCT
  AAAGAAGGCTTATGAAATTCAGAAGAACAAACCAAGAAATGATGATATTTTTAAGATAATTATGGCAATT
  GTGCTTTTCTTTTTCTTTTCCTGGATTCCCCACCAAATATTCACTTTTCTGGATGTATTGATTCAACTAG
  GCATCATACGTGACTGTAGAATTGCAGATATTGTGGACACGGCCATGCCTATCACCATTTGTATAGCTTA
  TTTTAACAATTGCCTGAATCCTCTTTTTTATGGCTTTCTGGGGAAAAAATTTAAAAGATATTTTCTCCAG
  CTTCTAAAATATATTCCCCCAAAAGCCAAATCCCACTCAAACCTTTCAACAAAAATGAGCACGCTTTCCT
  ACCGCCCCTCAGATAATGTAAGCTCATCCACCAAGAAGCCTGCACCATGTTTTGAGGTTGAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031850 unedited
  NGGGTCANAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACCAGGCAGCAGC
  GAGTGACAGGACGTCTGGACCGGCGCGCCGCTAGCAGCTCTGCCGGGCCGCGGCGGTGAT
  CGATGGGGAGCGGCTGGAGCGGACCCAGCGAGTGAGGGCGCACAGCCGGGACGCCGAGGC
  GGCGGGCGGGAGACCCGCACCAGCGCAGCCGGCCCTCGGCGGGACGTGACGCAGCGCCCG
  GGGCGCGGGTTTGATATTTGACAAATTGATCTAAAATGGCTGGGTTTTTATCTGAATAAC
  TCACTGATGCCATCCCAGAAAGTCGGCACCAGGTGTATTTGATATAGTGTTTGCAACAAA
  TTCGACCCAGGTGATCAAAATGATTCTCAACTCTTCTACTGAAGATGGTATTAAAAGAAT
  CCAAGATGATTGTCCCAAAGCTGGAAGGCATAATTACATATTTGTCATGATTCCTACTTT
  ATACAGTATCATCTTTGTGGTGGGAATATTTGGAAACAGCTTGGTGGTGATAGTCATTTA
  CTTTTATATGAAGCTGAAGACTGTGGCCAGTGTTTTTCTTTTGAATTTAGCACTGGCTGA
  CTTATGCTTTTTACTGACTTTGCCACTATGGGCTGTCTACACAGCTATGGAATACCGCTG
  GCCCTTTGGCAATTACCTATGTAAGATTGCTTCAGCCAGCGTCAGTTTCAACCTGTACGC
  TAGTGTGTTTCTACTCACGTGTCTCAGCATTGATCGATACCTGGCTATTGTTCACCCAAT
  GAAGTCCCGCCTTCGACGCACAATGCTTGTAGCCAAAGTCACCTGCATCATCATTTGGCT
  GCTGGCAGGCTTGGCCAGTTTGCCAGCTATNATCCATCGNAATGTATTTTTCATTGAGAA
  CACAATATTACAGTTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_031850 unedited
  TTCTATCGGGTCTTTATCTGATTCGCTTCGATCGCGGCCGCAATCTAAGAATCGGAAAAA
  AAAAAAAAAAAAAAAAAACAACTGGAAATAAACCACACCCACACCCTAGAGGCTAACATC
  AACCCCTGCCAGACCCCTTTTTATATTTACAGCAGTTTTTGATGCTCTAGACACACACAC
  ACATATATTGTGTAGAAAATATACCCCCTTTTAAACAGGAAGGCATACTTTTATGATATA
  TAATTCTTTTTACCACAAACTATATACACTTCTCAGATATTAAATCAAACTAATATACTA
  GGAAGACACTACTACTTGGGACCAGTGCAGCACCTTTACAAGTAGTTTAACTTTTATATG
  TGACTTTAGTAACACCATTATACAAATAAGCCATCTTACGGGCATTGTTTTGGCAGTGTA
  AACCTATAAGACACAGGTTTGAATTTCACAAAAAGATCTGCAACTTGACGACTACTGCTT
  AACATATCTTTGTACAAAATGTGCAGTACCAGGTGCAAGTGTAGCACAGTTGCTAATAGC
  TGAAAACCGGCACGAAAACTTTACTGCCCTTTGGAAACTGGACAGAACAATCTGGAACTC
  TCATCTCCTGTTGCTCCTCTAACGATTTAATATATTCTAAATACCTTTTATGTGGAAATA
  AAAAATAACAGGACAAAAGCGGCTAGGGGAGATTCCTTTTTCTGTCAGTTAAATTTTTCA
  AATCAACAATTCNTTCGAGTTTCTGACTTTGTTCTTCGAACAGCCGTCATCTGTCTATGG
  CAATGGGGGCTTTTCCTTTTGCCTTGTTGCAAAGGAAAGAAAGCTTTTGTTCAAACTTTT
  AAAAATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_031850
• Insert Size: 2650 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031850.1, NP_114038.1
• RefSeq Size: 2405 bp
• RefSeq ORF: 1080 bp
• Locus ID: 185
• Cytogenetics: 3q24
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction, Renin-angiotensin system, Vascular smooth muscle contraction
• Gene Summary: 'Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2020]'
  Transcript Variant: This variant (4), also known as pC3 or hAT1R-D, has an additional exon in the 5' region, which contains an in-frame AUG start codon, compared to variant 1. The resulting isoform (2) has a longer N-terminus, compared to isoform 1. Variants 3 and 4 encode the same isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.