CARS (NM_139273) Human Untagged Clone

CAT#: SC124342

CARS (untagged)-Human cysteinyl-tRNA synthetase (CARS), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CARS
• Synonyms: CARS1; CYSRS; MGC:11246
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124342 sequence for NM_139273 edited (data generated by NextGen Sequencing)
  ATGGCAGATTCCTCCGGGCAGCAGGGCAAAGGCCGGCGTGTGCAGCCCCAGTGGTCCCCT
  CCTGCTGGGACCCAGCCATGCAGACTCCACCTTTACAACAGCCTCACCAGGAACAAGGAA
  GTGTTCATACCTCAAGATGGGAAAAAGGTGACGTGGTATTGCTGTGGGCCAACCGTCTAT
  GACGCATCTCACATGGGGCACGCCAGGTCCTACATCTCTTTTGATATCTTGAGAAGAGTG
  TTGAAGGATTACTTCAAATTTGATGTCTTTTATTGCATGAACATTACGGATATTGATGAC
  AAGATCATCAAGAGGGCCCGGCAGAACCACCTGTTCGAGCAGTATCGGGAGAAGAGGCCT
  GAAGCGGCACAGCTCTTGGAGGATGTTCAGGCCGCCCTGAAGCCATTTTCAGTAAAATTA
  AATGAGACCACGGATCCCGATAAAAAGCAGATGCTCGAACGGATTCAGCACGCAGTGCAG
  CTTGCCACAGAGCCACTTGAGAAAGCTGTGCAGTCCAGACTCACGGGAGAGGAAGTCAAC
  AGCTGTGTGGAGGTGTTGCTGGAAGAAGCCAAGGATTTGCTCTCTGACTGGCTGGATTCT
  ACACTTGGCTGTGATGTCACTGACAATTCCATCTTCTCCAAGCTGCCCAAGTTCTGGGAG
  GGGGACTTCCACAGAGACATGGAAGCTCTGAATGTTCTCCCTCCAGATGTCTTAACCCGG
  GTTAGTGAGTATGTGCCAGAAATTGTGAACTTTGTCCAGAAGATTGTGGACAACGGTTAC
  GGCTATGTCTCCAATGGGTCTGTCTACTTTGATACAGCGAAGTTTGCTTCTAGCGAGAAG
  CACTCCTATGGGAAGCTGGTGCCTGAGGCCGTTGGAGATCAGAAAGCCCTTCAAGAAGGG
  GAAGGTGACCTGAGCATCTCTGCAGACCGCCTGAGTGAGAAGCGCTCTCCCAACGACTTT
  GCCTTATGGAAGGCCTCTAAGCCCGGAGAACCGTCCTGGCCGTGCCCTTGGGGAAAGGGT
  CGTCCGGGCTGGCATATCGAGTGCTCGGCCATGGCAGGCACCCTCCTAGGGGCTTCGATG
  GACATTCACGGAGGTGGGTTCGACCTCCGGTTCCCCCACCATGACAATGAGCTGGCACAG
  TCGGAGGCCTACTTTGAAAACGACTGCTGGGTCAGGTACTTCCTGCACACAGGCCACCTG
  ACCATTGCAGGCTGCAAAATGTCAAAGTCACTAAAAAACTTCATCACCATTAAAGATGCC
  TTGAAAAAGCACTCAGCACGGCAGTTGCGGCTGGCCTTCCTCATGCACTCGTGGAAGGAC
  ACCCTGGACTACTCCAGCAACACCATGGAGTCAGCGCTTCAATATGAGAAGTTCTTGAAT
  GAGTTTTTCTTAAATGTGAAAGATATCCTTCGCGCTCCTGTTGACATCACTGGTCAGTTT
  GAGAAGTGGGGAGAAGAAGAAGCAGAACTGAATAAGAACTTTTATGACAAGAAGACAGCA
  ATTCACAAAGCCCTCTGTGACAATGTTGACACCCGCACCGTCATGGAAGAGATGCGGGCC
  TTGGTCAGTCAGTGCAACCTCTATATGGCAGCCCGGAAAGCCGTGAGGAAGAGGCCCAAC
  CAGGCTCTGCTGGAGAACATCGCCCTGTACCTCACCCATATGCTGAAGATCTTTGGGGCC
  GTAGAAGAGGACAGCTCCCTGGGATTCCCGGTCGGAGGGCCTGGAACCAGCCTCAGTCTC
  GAGGCCACAGTCATGCCCTACCTTCAGGTGTTATCAGAATTCCGAGAAGGAGTGCGGAAG
  ATTGCCCGAGAGCAAAAAGTCCCTGAGATTCTGCAGCTCAGCGATGCCCTGCGGGACAAC
  ATCCTGCCCGAGCTTGGGGTGCGGTTTGAAGACCACGAAGGACTGCCCACAGTGGTGAAA
  CTGGTAGACAGAAACACCTTATTAAAAGAGAGAGAAGAAAAGAGACGGGTTGAAGAGGAG
  AAGAGGAAGAAGAAAGAGGAGGCGGCCCGGAGGAAACAGGAACAAGAAGCAGCAAAGCTG
  GCCAAGATGAAGATTCCCCCCAGTGAGATGTTCTTGTCAGAAACCGACAAATACTCCAAG
  TTTGATGAAAATGTAAGCATGGTCTGCCCACACATGACATGGAGGGCAAAGAGCTCAGCA
  AAGGGCAAGCCAAGAAGCTGA
  
  Clone variation with respect to NM_139273.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_139273 unedited
  AGGAATTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGTAGCCGCAG
  CTGCGGGTCCGGGATTCCCAGCCATGGCAGATTCCTCCGGGCAGCAGGGCAAAGGCCGGC
  GTGTGCAGCCCCAGTGGTCCCCTCCTGCTGGGACCCAGCCATGCAGACTCCACCTTTACA
  ACAGCCTCACCAGGAACAAGGAAGTGTTCATACCTCAAGATGGGAAAAAGGTGACGTGGT
  ATTGCTGTGGGCCAACCGTCTATGACGCATCTCACATGGGGCACGCCAGGTCCTACATCT
  CTTTTGATATCTTGAGAAGAGTGTTGAAGGATTACTTCAAATTTGATGTCTTTTATTGCA
  TGAACATTACGGATATTGATGACAAGATCATCAAGAGGGCCCGGCAGAACCACCTGTTCG
  AGCAGTATCGGGAGAAGAGGCCTGAAGCGGCACAGCTCTTGGAGGATGTTCAGGCCGCCC
  TGAAGCCATTTTCAGTAAAATTAAATGAGACCACGGATCCCGATAAAAAGCAGATGCTCG
  AACGGATTCAGCACGCAGTGCAGCTTGCCACAGAGCCACTTGAGAAAGCTGTGCAGTCCA
  GACTCACGGGAGAGGAAGTCAACAGCTGTGTGGAGGTGTTGCTGGAAGAAGCCAAGGATT
  TGCTCTCTGACTGGCTGGATTCTACACTTGGCTGTGATGTCACTGACAATTCCATCTTCT
  CCAAGCTGCCCAAGTTCTGGGAGGGGGACTTCCACAGAGACATGGAAGCTCTGAAATGTT
  CTCCTCCAGATGTCTTAACCCGGGTTAGTGAGTATGTGCCAGAAATTGTGAACTTGTCCA
  GAAGAATGTGACACGGTTACGCTATGTCTCCAATGGTCTGTCTACTTGAACAGCGAAGTT
  GCTTCTACGAGAGCACTCTATGAAGNCTGTGCTGAGGCGTTGNAATCAAAAGCCTCAGAG
  GGGAGTGACTGACATTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_139273 unedited
  TTTTTTTCGTTTACTAAGAACCGCGGCCGCATACNANGATCGNATTTTTTTTTTTTTTTT
  TTCACTGTTNGAAAAAAATTATTATCAATGTCTCAAAGCCAACGACGACACGAACCTACA
  TGAACACAACTCTTAATTTAGGACCCAAGGGTGACTGTAAACATGATAGGAGCGCTGGGA
  CATTGTCACTGAGGCAGACAGCAGCCACTAGTCCACAATGGTTTAAAAAGTCAGTCCTGT
  GCCCCCTCACTGGAAGCTTCCATTCTGGGCCATCTGCAGATATTCCTTGTAGAGCTTCTC
  CTGAGCCTCGAAGAGCTTCTTCAGCTTCTTGGCTTGCCCTTTGCTGAGCTCTTTGCCCTC
  CATGTCATGTGTGGGCAGACCATGCTTACATTTTCATCAAACTTGGAGTATTTGTCGGTT
  TCTGACAAGAACATCTCACTGGGGGGAATCTTCATCTTGGCCAGCTTTGCTGCTTCTTGT
  TCCTGTTTCCTCCGGGCCGCCTCCTCTTTCTTCTTCCTCTTCTCCTCTTCAACCCGTCTC
  TTTTCTTCTCTCTCTTTTAATAAGGTGTTTCTGTCTACCAGTTTCACCACTGTGGGCAGT
  CCTTCGTGGTCTTCAAACCGCACCCCAAGCTCGGGCAGGATGTTGTCCCGCAGGGCATCG
  CTGAGCTGCAGAATCTCAGGGACTTTTTGCTCTCGGGCAATCTTCCGCACTCCTTCTCGG
  AATTCTGATAACACCNTGAGGTAGGGCATGACTGTGGCCTCGAGACTGAGGCTGGTTCCA
  GGCCCTCCGACCGGGAATCCCAGGGAGCTGTCCTCTTCTACGGCCCCAAAGTCTTCACAT
  ATGGGTGAAGTACAGGGCGATGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_139273
• Insert Size: 2550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139273.2, NP_644802.1
• RefSeq Size: 2536 bp
• RefSeq ORF: 2181 bp
• Locus ID: 833
• Cytogenetics: 11p15.4
• Domains: tRNA-synt_1e
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• Gene Summary: 'This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2010]'
  Transcript Variant: This variant (1) consists of 22 exons and encodes the shortest isoform (a).