GIT2 (NM_057169) Human Untagged Clone

CAT#: SC124350

GIT2 (untagged)-Human G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GIT2
• Synonyms: CAT-2; CAT2; PKL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_057169 edited
  ATGTCGAAACGGCTCCGGAGCAGCGAGGTGTGCGCTGACTGCAGCGGGCCGGATCCTTCC
  TGGGCATCAGTAAATAGGGGAACGTTTTTATGTGATGAGTGCTGCAGTGTCCATCGGAGT
  CTAGGGCGCCATATCTCCCAAGTGAGGCATCTGAAACACACACCGTGGCCTCCAACACTG
  CTTCAGATGGTTGAGACCTTGTATAATAACGGTGCTAACTCTATATGGGAGCATTCTTTG
  CTGGACCCTGCGTCTATTATGAGTGGAAGACGTAAAGCTAATCCACAGGATAAAGTACAT
  CCCAATAAAGCGGAATTCATCAGAGCCAAGTATCAGATGTTAGCGTTCGTCCATCGCTTG
  CCCTGCCGGGATGACGATAGTGTGACTGCCAAAGATCTTAGCAAGCAACTCCATTCGAGC
  GTGAGAACAGGGAATCTTGAAACCTGTTTGAGACTGTTATCTTTAGGAGCACAAGCCAAC
  TTCTTTCATCCTGAAAAAGGAAACACCCCACTCCATGTTGCCTCCAAAGCAGGGCAGATT
  TTACAGGCTGAATTATTGGCAGTATATGGAGCAGACCCAGGCACACAGGATTCTAGTGGG
  AAAACTCCCGTTGATTATGCAAGGCAAGGAGGGCACCATGAGCTGGCAGAGCGCCTCGTG
  GAAATACAGTATGAGCTAACGGACAGACTAGCCTTCTATCTCTGTGGCAGGAAACCAGAT
  CACAAAAATGGACAGCACTTTATAATACCTCAAATGGCAGACAGACGGCTCAGCCTGGAT
  TTGTCTGAATTGGCAAAAGCTGCTAAGAAGAAACTTCAATCTCTAAGTAATCATTTGTTT
  GAAGAACTTGCCATGGATGTGTACGATGAAGTTGACAGGCGAGAGACGGATGCAGTCTGG
  CTTGCCACGCAAAACCACAGCGCCCTGGTAACCGAGACAACGGTCGTCCCCTTTCTTCCG
  GTCAATCCTGAGTACTCATCAACACGAAATCAGGGCAGACAGAAGTTAGCTCGGTTCAAC
  GCCCATGAGTTTGCCACGCTGGTCATTGACATTCTCAGTGACGCCAAGAGGAGACAGCAG
  GGCAGTTCTCTCTCGGGTTCAAAAGACAATGTGGAGCTCATACTGAAAACCATCAATAAC
  CAGCACAGCGTTGAGAGTCAAGACAACGATCAGCCCGACTATGACAGCGTGGCATCAGAC
  GAAGACACAGATTTGGAAACCACTGCAAGCAAAACAAACCGGCAGAAGAGCCTAGATTCA
  GATTTATCAGATGGACCAGTCACTGTACAGGAATTTATGGAGGTCAAAAACGCTCTAGTG
  GCTTCTGAGGCCAAGATACAGCAGCTAATGAAGGTGAATAACAACTTGAGTGACGAGCTG
  AGAATTATGCAGAAAAAGCTTCAAACACTCCAGAGTGAAAATTCGAACCTCAGGAAACAG
  GCCACAACCAATGTATATCAGGTGCAAACTGGTTCTGAGTACACAGACACTTCCAACCAC
  TCTTCCTTAAAGAGACGTCCGTCTGCCCGGGGCAGTAGGCCCATGTCCATGTACGAGACC
  GGATCAGGTCAGAAACCATATCTCCCAATGGGAGAAGCGAGCCGCCCCGAAGAGAGCAGG
  ATGAGACTCCAGCCCTTCCCCGCGCACGCATCCAGGCTGGAGAAGCAGAACAGCACACCT
  GAGAGTGACTACGACAACACTCCCAACGACATGGAGCCAGATGGCATGGGGTCAAGCCGA
  AAGGGACGGCAAAGAAGTATGGTGTGGCCAGGGGATGGCTTGGTACCAGACACAGCAGAA
  CCCCATGTGGCCCCAAGCCCCACTCTCCCTAGCACCGAAGATGTCATCAGGAAGACTGAA
  CAGATCACCAAAAACATACAGGAGCTCTTAAGAGCAGCCCAAGAAAATAAACATGACAGA
  AACCCAAGTCTGATATGGTGAGGACTTCCCTTCGTTTACTGACGTCCAGTGCCTACCGAC
  TGCAGTCAGAGTGCAAGAAGACCCTCCCAGGGGACCCCGGCTCACCCACAGACGTTCAGC
  TGGTCACGCAGCAGGTCATCCAGTGTGCGTACGACATCGCCAAGGCTGCCAAGCAGCTGG
  TTACCATCACCACCAAAGAGAACAACAACTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_057169 unedited
  TTGTACACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGTCAGCGCCGCCG
  CAGCTGGGACCCGTTAGAGCGGAAGCGCCGCCGCCACCGCCGCCTTTGCTGTCCCCCGGC
  CTCTAGTTCCCCGCAGGTGGGAGGTGGGAGCCATGTCGAAACGGCTCCGGAGCAGCGAGG
  TGTGCGCTGACTGCAGCGGGCCGGATCCTTCCTGGGCATCAGTAAATAGGGGAACGTTTT
  TATGTGATGAGTGCTGCAGTGTCCATCGGAGTCTAGGGCGCCATATCTCCCAAGTGAGGC
  ATCTGAAACACACACCGTGGCCTCCAACACTGCTTCAGATGGTTGAGACCTTGTATAATA
  ACGGTGCTAACTCTATATGGGAGCATTCTTTGCTGGACCCTGCGTCTATTATGAGTGGAA
  GACGTAAAGCTAATCCACAGGATAAAGTACATCCCAATAAAGCGGAATTCATCAGAGCCA
  AGTATCAGATGTTAGCGTTCGTCCATCGCTTGCCCTGCCGGGATGACGATAGTGTGACTG
  CCAAAGATCTTAGCAAGCAACTCCATTCGAGCGTGAGAACAGGGAATCTTGAAACCTGTT
  TGAGACTGTTATCTTTAGGAGCACAAGCCAACTTCTTTCATCCTGAAAAAGGAAACACCC
  CACTCCATGTTGCCTCCAAAGCAGGGCAGATTNTACAGGCTGAATTATTGGCAGTATATG
  GAGCAGACCCAGGCACACAGGATTCTAGTGGGAAAACTCCCGTTGATTATGCANGGGCAG
  GGAGGCACCATGAGCTGGCAGAGCGCCTCGTGGAATACAGTATGAGCTAACGGACAGACT
  AGCCTTCTATCTCTGTGGCAGGANACCAGATCACANAATGGACAGCACTTTNATATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_057169 unedited
  AATTTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTGTTTTATGAAAATATTTCATTTATAG
  TTTCAGCCATAGGCAGGGAGTTTTAAGGGATGGAAAAGTTTTCACAAACTTTAGACAATG
  AAACTATACTTTTGGATGTATGTGATCAACTCAACAGTTGTTGACAACACAACCGAACAT
  CAGAAACTAAACCAGCAAATAGGCACAAAATAAGGCAAGTGGGTTAAATAGTTGAAAATT
  GGTTAGAAAACATGCAAAAATAATACTGAGTTTTCTTTTAAAAAGTTTTAAACCGTCATT
  AAATGTTTCTTTTTGTAGAAATCTGAAGAGTTCTGCGTCTGAATTTGAAATTGGACTTTA
  TAAAGCCTAGAAAACAGAGGAGGCGGTGCCCTGCCCTTGTCAGTTGTTGTTCTCTTTGGT
  GGTGATGGTAACCAGCTGCTTGGCAGCCTTGGCGATGTCGTACGCACACTGGATGACCTG
  CTGCGTGACCAGCTGAACGTCTGTGGGTGAGCCGGGGTCCCCTGGGAGGGTCTTCTTGCA
  CTCTGACTGCAGTCGGTAGGCACTGGACGTCAGTAAACGAAGGGAAGTCCTCACCATATC
  AGACTTGGGTTTCTGTCATGTTTATTNTCTTGGGCTGCTCCTAAGAGCTCCTGTATGTTT
  TTGGTGATCTGTTCAGTCTTCCTGATGACATCCTTCGTGCCTAGGAAAAGTGGGCTTGGG
  GCCCCATGGGGTTCTGCTGTGTTTGTTACCAGCCATCCCCTGGCCAACCATACTTTTTGN
  CGCCCTTTCCGGTTGACCCATGCCATTTGCCTCATGTCGTGGGAATGTTGCCTAACACCT
  CTCAGGTGCCTGTCTGTTCTCCACCTGTATCCTTCCCCGGAAAGCTCGANTCTATCCTGG
  TTTTTCGGGGGGGTGTTTTTCCATTGGAAGAAAGGTTTTGACCTATCCGCTCTTAACTGC
  AAGGGCCACTGCCCCGCCAAAAGAGTTTTAAGAAAAAGGTGAAGGCCGGTTCCAAAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_057169
• ORF Size: 2280 bp
• Insert Size: 2820
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_057169.2, NP_476510.1
• RefSeq Size: 5525
• RefSeq ORF: 2280
• Locus ID: 9815
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis
• Gene Summary: This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq, Sep 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).