MBD1 (NM_015844) Human Untagged Clone

CAT#: SC124480

MBD1 (untagged)-Human methyl-CpG binding domain protein 1 (MBD1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MBD1
• Synonyms: CXXC3; PCM1; RFT
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_015844 edited
  TGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGG
  GGGAGGTGCAGGAGGGACCCTCGCCATGGGTCCACGGGCCTAGAGTGGCGGAAGATACCG
  GCCTGGTGCCAAACTGGCTACTGCTGCTTCCTGTGGCCTCCATGGCTGAGGACTGGCTGG
  ACTGCCCGGCCCTGGGCCCTGGCTGGAAGCGCCGCGAAGTCTTTCGCAAGTCAGGGGCCA
  CCTGTGGACGCTCAGACACCTATTACCAGAGCCCCACAGGAGACAGGATCCGAAGCAAAG
  TTGAGCTGACTCGATACCTGGGCCCTGCGTGTGATCTCACCCTCTTCGACTTCAAACAAG
  GCATCTTGTGCTATCCAGCCCCCAAGGCCCATCCCGTGGCGGTTGCCAGCAAGAAGCGAA
  AGAAGCCTTCAAGGCCAGCCAAGACTCGGAAACGTCAGGTTGGACCCCAGAGTGGTGAGG
  TCAGGAAGGAGGCCCCGAGGGATGAGACCAAGGCTGACACTGACACAGCCCCAGCTTCAT
  TCCCTGCTCCTGGGTGCTGTGAGAACTGTGGAATCAGCTTCTCAGGGGATGGCACCCAAA
  GGCAGCGGCTCAAAACGTTGTGCAAAGACTGTCGAGCACAGAGAATTGCCTTCAACCGGG
  AACAGAGAATGTTTAAGCGTGTGGGCTGTGGGGAGTGTGCAGCCTGCCAGGTAACAGAAG
  ACTGTGGGGCCTGCTCCACCTGCCTCCTGCAGCTGCCCCATGATGTGGCATCGGGGCTGT
  TCTGCAAGTGTGAACGGAGACGCTGCCTCCGGATTGTGGAAAGGAGCCGAGGGTGTGGAG
  TATGCCGGGGCTGTCAGACCCAAGAGGATTGTGGCCATTGCCCCATCTGCCTTCGCCCTC
  CCCGCCCTGGTCTCAGGCGCCAGTGGAAATGTGTCCAGCGACGTTGCCTACGGGGTAAAC
  ATGCCCGCCGCAAGGGAGGCTGTGACTCCAAGATGGCTGCCAGGCGGCGCCCCGGAGCCC
  AGCCACTGCCTCCACCACCCCCATCACAGTCCCCAGAGCCCACAGAGCCGCACCCCAGAG
  CCCTGGCCCCCTCGCCACCTGCCGAGTTCATCTATTACTGTGTAGACGAGGACGAGCTAA
  AGCGGCTGCTGCCCAGTGTCTGGTCAGAGTCTGAGGATGGGGCAGGATCGCCCCCACCTT
  ACCGTCGTCGAAAGAGGCCCAGCTCTGCCCGACGGCACCATCTTGGCCCTACCTTGAAGC
  CCACCTTGGCTACACGCACAGCCCAACCAGACCATACCCAGGCTCCAACGAAGCAGGAAG
  CAGGTGGTGGCTTTGTGCTGCCCCCGCCTGGCACTGACCTTGTGTTTTTACGGGAAGGCG
  CAAGCAGTCCTGTGCAGGTGCCGGGCCCTGTTGCAGCTTCCACAGAAGCCCTGTTGCAGG
  AGGCCCAGTGCTCTGGCCTGAGTTGGGTTGTGGCCTTACCCCAGGTGAAGCAAGAGAAGG
  CGGATACCCAGGACGAGTGGACACCAGGCACAGCTGTCCTGACTTCTCCCGTATTGGTGC
  CTGGCTGCCCTAGCAAGGCAGTAGACCCAGGCCTGCCTTCTGTGAAGCAAGAGCCACCTG
  ACCCAGAGGAGGACAAGGAGGAGAACAAGGATGATTCTGCCTCCAAATTGGCCCCAGAGG
  AAGAGGCAGGAGGGGCTGGCACACCCGTGATCACGGAGATTTTCAGCCTGGGTGGAACCC
  GCTTCCGAGATACAGCAGTCTGGTTGCCAAGGTCCAAAGACCTTAAAAAACCTGGAGCTA
  GAAAGCAGTAGACTGGAGGCTTCTACAGACTGTAGGATTCAAG:::::::GGCAGGCACT
  CGGGAAGGGAAGATGGATGTAAAGTGTGGGAGACCGAGGACACAGTGGAGCCCACGAGCA
  CGAGCTGGAACCCACGAGGATGGCCTGGAACCCATGTCAGTCTCTCACCACCTCCAGCTT
  CGATGATGTGGGTGTCCTGCAGAAGAAGCTGGTGCCCTTCCTCACAGAGTTAAATATGCA
  TCTGGCCCAGGAATTAGAGAAGCTGAAAGGATGATCCTGGGGAAGGTGGAGCAGCTGCAG
  GCCTGGCTGCAGGCCTGACTACTGCCCACACCAACGAGGTGATCTAGCAGATACATGGCA
  ACGTGTGAACTGCAACAACGCCTGGTGCCCCAGCACCAACCTTCCAAGTGTAAAAACAAT
  GTGCTGCTGCTTCACTTCCGCCCTCCGGTTATCAAGCAAAATGTCTCTTGTGGCCCATCT
  TACTGGAAGAGAGTTCCGGGAAACATAGCCTCACCAAGGTGACACATTACAAAGCCACCC
  TACCATGAATCCGCTCCCAAGGGTCTCACTGCTCACCTGAGGATAACTCAATATAACTAT
  GTTGCTGAAAATGCAAAGCTGAAGACCATGGATTTCATGGTGATTCCAGCAAGTACAGAG
  ATTCTATGAAGCCCACCCAGAAAAAACTTGCTGGTCCTGGCTATTTTTGTGTCATTTATT
  CAAGTATTGAGAACCTGGCCTGTGGTAGGCACTGTACTTAATACTAGGATACAGAAATGC
  AAAAGATACGGCCCATGCAATTTTATTAAATGCATCAATATGTATTACAAAAAAAAAAAA
  AAAAAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015844
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_015844.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015844.1, NP_056669.1
• RefSeq Size: 2793 bp
• RefSeq ORF: 1650 bp
• Locus ID: 4152
• Cytogenetics: 18q21.1
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Feb 2011]'
  Transcript Variant: This variant (3) lacks an in-frame coding exon compared to variant 1, resulting in a shorter isoform (3) missing an internal protein segment compared to isoform 1.