HLAB (HLA-B) (NM_005514) Human Untagged Clone

CAT#: SC124484

HLA (untagged)-Human major histocompatibility complex, class I, B (HLA-B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HLA-B
• Synonyms: AS; B-4901; HLAB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124484 sequence for NM_005514 edited (data generated by NextGen Sequencing)
  ATGCTGGTCATGGCGCCCCGAACCGTCCTCCTGCTGCTCTCGGCGGCCCTGGCCCTGACC
  GAGACCTGGGCCGGCTCCCACTCCATGAGGTATTTCTACACCTCCGTGTCCCGGCCCGGC
  CGCGGGGAGCCCCGCTTCATCTCAGTGGGCTACGTGGACGACACCCAGTTCGTGAGGTTC
  GACAGCGACGCCGCGAGTCCGAGAGAGGAGCCGCGGGCGCCGTGGATAGAGCAGGAGGGG
  CCGGAGTATTGGGACCGGAACACACAGATCTACAAGGCCCAGGCACAGACTGACCGAGAG
  AGCCTGCGGAACCTGCGCGGCTACTACAACCAGAGCGAGGMCGGGTCTCACACCCTCCAG
  AGCATGTACGGCTGCGACGTGGGGCCGGACGGGCGCCTCCTCCGCGGGCATGACCAGTAC
  GCCTACGACGGCAAGGATTACATCGCCCTGAACGAGGACCTGCGCTCCTGGACCGCCGCG
  GACACGGCGGCTCAGATCACCCAGCGCAAGTGGGAGGCGGCCCGTGAGGCGGAGCAGYGG
  AGAGCCTACCTGGAGGGCGAGTGCGTGGAGTGGCTCCGCAGATACCTGGAGAACGGGAAG
  GACAAGCTGGAGCGCGCTGACCCCCCAAAGACACACGTGACCCACCAYCCCRTCTCTGAC
  CATGAGGCCACCCTGAGGTGCTGGGCCCTGGGYTTCTACCCTGCGGAGATCACACTGACC
  TGGCAGCGGGATGGCGAGGACCAAACTCAGGACACTGAGCTTGTGGAGACCAGACCAGCA
  GGAGATAGAACCTTCCAGAAGTGGGCAGCTGTGGTGGTGCCTTCTGGAGAAGAGCAGAGA
  TACACATGCCATGTACAGCATGAGGGGCTGCCGAAGCCCCTCACCCTGAGATGGGAGCCG
  TCTTCCCAGTCCACCGTCCCCATCGTGGGCATTGTTGCTGGCCTGGCTGTCCTAGCAGTT
  GTGGTCATCGGAGCTGTGGTCGCTGCTGTGATGTGTAGGAGGAAGAGTTCAGGTGGAAAA
  GGAGGGAGCTACTCTCAGGCTGCGTGCAGCGACAGTGCCCAGGGCTCTGATGTGTCTCTC
  ACAGCTTGA
  
  Clone variation with respect to NM_005514.6
  341 c=>m;538 c=>y;648 c=>y;652 a=>r;693 t=>y
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005514 unedited
  CAATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGAGATGCTG
  GTCATGGCGCCCCGAACCGTCCTCCTGCTGCTCTCGGCGGCCCTGGCCCTGACCGAGACC
  TGGGCCGGCTCCCACTCCATGAGGTATTTCTACACCTCCGTGTCCCGGCCCGGCCGCGGG
  GAGCCCCGCTTCATCTCAGTGGGCTACGTGGACGACACCCAGTTCGTGAGGTTCGACAGC
  GACGCCGCGAGTCCGAGAGAGGAGCCGCGGGCGCCGTGGATAGAGCAGGAGGGGCCGGAG
  TATTGGGACCGGAACACACAGATCTACAAGGCCCAGGCACAGACTGACCGAGAGAGCCTG
  CGGAACCTGCGCGGCTACTACAACCAGAGCGAGGCCGGGTCTCACACCCTCCAGAGCATG
  TACGGCTGCGACGTGGGGCCGGACGGGCGCCTCCTCCGCGGGCATGACCAGTACGCCTAC
  GACGGCAAGGATTACATCGCCCTGAACGAGGACCTGCGCTCCTGGACCGCCGCGGACACG
  GCGGCTCAGATCACCCAGCGCAAGTGGGAGGCGGCCCGTGAGGCGGAGCAGCGGAGAGCC
  TACCTGGAGGGCGAGTGCGTGGAGTGGCTCCGCAGATACCTGGAGAACGGGAAGGACAAG
  CTGGAGCGCGCTGACCCCCCAAAGACACACGTGACCCACCACCCCATCTCTGACCCATGA
  GGCCACCCTGAGTGCTGGGCCCTGGGTTTCTACCCTGCNGAGATCACACTGACCTGGCAG
  CGGGATGGCGAGGACCAAACTCANGACACTNGAGCTTGTGGAGACCCAGACCAGCANGAG
  ATAGAACCTTCCANAAGNNTGGCAGCTGTGGTGGNTGCCTTNCTGAGAAGAGCAGAGATA
  CACATGCCATGTACAGCATGAGGGGCTGCCGAGCCCCTCACCTGAGAGGNNAGCGTNNTC
  CCAGTCACGTNCCATCGTGGGCATTGTGCTGGCCTGCTGCCTANCANTGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005514 unedited
  NGGTACGACTATGGNACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTGA
  AGGTTCTCAGGTCTTTATTTGCTCTCTCAAATTCCAGGAATTGACTTATTTAATTAATCC
  ATCAACCTCTCATAGCAAATATTTGAGAAAACAAATTTATATTCAGATTCTTATTTTCAG
  TAGGGAAGTAAGAAGTTGCAGCTCAGTGCACGTAAAGTTGAGACAGAGATGGAGACATCC
  AGCCCCACCTCTCTGGAACAAGAAAGATGACTGGGGAGGAAACACAGGTCAGCATGGGAA
  CAGGGGTCACAGTGGACACAAGGGTGGGCTGTCTCTCCACCTCCTCACATTATGCTAACA
  GGGACGCAGACACATTCAGGTGCCTTTGCAGAAAGAGATGCCAGAGGCTCTTGAAGTCAC
  AAAGGGGAGGCGTGAAGAAATCCTGCATCTCAGTCCCTCACAAGACAGCTGTCTCAGGCT
  TTTCAAGCTGTGAGAGACACATCAGAGCCCTGGGCACTGTCGCTGCACGCAGCCTGAGAG
  TAGCTCCCTCCTTTTCCACCTGAACTCTTCCTCCTACACATCACAGCAGCGACCACAGCT
  CCGATGACCACAACTGCTAGGACAGCCAGGCCAGCAACAATGCCCACGATGGGGACGGTG
  GACTGGGAAGACGGCTCCCTCTAGGTGGAGGGGGGCTTCGGCAGCCCCTCATGCTGTACA
  TGGCATGTGTATCTCTGCTCTTCTCCAGAAGCACCACACAGCTGCCCACTCTGGAAGGGT
  CTATCTCCTGGTGGTCTGGTCTCCCCAGCTCATGTCCTGAGTTTGGTCCTCGCCATCCGC
  TGCCAGTCAGTGTGATCTCCGCAGGGTAGAAACCCAGGGCCAGCACCTTCAGGTGGCCCT
  CTGGTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005514
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005514.5, NP_005505.2
• RefSeq Size: 1533 bp
• RefSeq ORF: 1089 bp
• Locus ID: 3106
• Cytogenetics: 6p21.33
• Domains: MHC_I, ig, IGc1
• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Endocytosis, Graft-versus-host disease, Natural killer cell mediated cytotoxicity, Type I diabetes mellitus, Viral myocarditis
• Gene Summary: 'HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008]'