DNMT3B (NM_175848) Human Untagged Clone

CAT#: SC124706

DNMT3B (untagged)-Human DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNMT3B
• Synonyms: ICF; ICF1; M.HsaIIIB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_175848, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGGGAGACACCAGGCATCTCAATGGAGAGGAGGACGCCGGCGGGAGGGAAGACTCGATCCTCGTCA
  ACGGGGCCTGCAGCGACCAGTCCTCCGACTCGCCCCCAATCCTGGAGGCTATCCGCACCCCGGAGATCAG
  AGGCCGAAGATCAAGCTCGCGACTCTCCAAGAGGGAGGTGTCCAGTCTGCTAAGCTACACACAGGACTTG
  ACAGGCGATGGCGACGGGGAAGATGGGGATGGCTCTGACACCCCAGTCATGCCAAAGCTCTTCCGGGAAA
  CCAGGACTCGTTCAGAAAGCCCAGCTGTCCGAACTCGAAATAACAACAGTGTCTCCAGCCGGGAGAGGCA
  CAGGCCTTCCCCACGTTCCACCCGAGGCCGGCAGGGCCGCAACCATGTGGACGAGTCCCCCGTGGAGTTC
  CCGGCTACCAGGTCCCTGAGACGGCGGGCAACAGCATCGGCAGGAACGCCATGGCCGTCCCCTCCCAGCT
  CTTACCTTACCATCGACCTCACAGACGACACAGAGGACACACATGGGACGCCCCAGAGCAGCAGTACCCC
  CTACGCCCGCCTAGCCCAGGACAGCCAGCAGGGGGGCATGGAGTCCCCGCAGGTGGAGGCAGACAGTGGA
  GATGGAGACAGTTCAGAGTATCAGGATGGGAAGGAGTTTGGAATAGGGGACCTCGTGTGGGGAAAGATCA
  AGGGCTTCTCCTGGTGGCCCGCCATGGTGGTGTCTTGGAAGGCCACCTCCAAGCGACAGGCTATGTCTGG
  CATGCGGTGGGTCCAGTGGTTTGGCGATGGCAAGTTCTCCGAGGTCTCTGCAGACAAACTGGTGGCACTG
  GGGCTGTTCAGCCAGCACTTTAATTTGGCCACCTTCAATAAGCTCGTCTCCTATCGAAAAGCCATGTACC
  ATGCTCTGGAGAAAGCTAGGGTGCGAGCTGGCAAGACCTTCCCCAGCAGCCCTGGAGACTCATTGGAGGA
  CCAGCTGAAGCCCATGTTGGAGTGGGCCCACGGGGGCTTCAAGCCCACTGGGATCGAGGGCCTCAAACCC
  AACAACACGCAACCAGAGAACAAGACTCGAAGACGCACAGCTGACGACTCAGCCACCTCTGACTACTGCC
  CCGCACCCAAGCGCCTCAAGACAAATTGCTATAACAACGGCAAAGACCGAGGGGATGAAGATCAGAGCCG
  AGAACAAATGGCTTCAGATGTTGCCAACAACAAGAGCAGCCTGGAAGATGGCTGTTTGTCTTGTGGCAGG
  AAAAACCCCGTGTCCTTCCACCCTCTCTTTGAGGGGGGGCTCTGTCAGACATGCCGGGATCGCTTCCTTG
  AGCTGTTTTACATGTATGATGACGATGGCTATCAGTCTTACTGCACTGTGTGCTGCGAGGGCCGAGAGCT
  GCTGCTTTGCAGCAACACGAGCTGCTGCCGGTGTTTCTGTGTGGAGTGCCTGGAGGTGCTGGTGGGCACA
  GGCACAGCGGCCGAGGCCAAGCTTCAGGAGCCCTGGAGCTGTTACATGTGTCTCCCGCAGCGCTGTCATG
  GCGTCCTGCGGCGCCGGAAGGACTGGAACGTGCGCCTGCAGGCCTTCTTCACCAGTGACACGGGGCTTGA
  ATATGAAGCCCCCAAGCTGTACCCTGCCATTCCCGCAGCCCGAAGGCGGCCCATTCGAGTCCTGTCATTG
  TTTGATGGCATCGCGACAGGCTACCTAGTCCTCAAAGAGTTGGGCATAAAGGTAGGAAAGTACGTCGCTT
  CTGAAGTGTGTGAGGAGTCCATTGCTGTTGGAACCGTGAAGCACGAGGGGAATATCAAATACGTGAACGA
  CGTGAGGAACATCACAAAGAAAAATATTGAAGAATGGGGCCCATTTGACTTGGTGATTGGCGGAAGCCCA
  TGCAACGATCTCTCAAATGTGAATCCAGCCAGGAAAGGCCTGTATGAGGGTACAGGCCGGCTCTTCTTCG
  AATTTTACCACCTGCTGAATTACTCACGCCCCAAGGAGGGTGATGACCGGCCGTTCTTCTGGATGTTTGA
  GAATGTTGTAGCCATGAAGGTTGGCGACAAGAGGGACATCTCACGGTTCCTGGAGTGTAATCCAGTGATG
  ATTGATGCCATCAAAGTTTCTGCTGCTCACAGGGCCCGATACTTCTGGGGCAACCTACCCGGGATGAACA
  GGCCCGTGATAGCATCAAAGAATGATAAACTCGAGCTGCAGGACTGCTTGGAATACAATAGGATAGCCAA
  GTTAAAGAAAGTACAGACAATAACCACCAAGTCGAACTCGATCAAACAGGGGAAAAACCAACTTTTCCCT
  GTTGTCATGAATGGCAAAGAAGATGTTTTGTGGTGCACTGAGCTCGAAAGGATCTTTGGCTTTCCTGTGC
  ACTACACAGACGTGTCCAACATGGGCCGTGGTGCCCGCCAGAAGCTGCTGGGAAGGTCCTGGAGCGTGCC
  TGTCATCCGACACCTCTTCGCCCCTCTGAAGGACTACTTTGCATGTGAATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_175848 unedited
  TTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGGAGATTCGCGAGCCCA
  GCGCCCTGCACGGCCGCCAGCCGGCCTCCCGCCAGCCAGCCCCGACCCGCGGCTCCGCCG
  CCCAGCCGCGCCCCAGCCAGCCCTGCGGCAGGAAAGCATGAAGGGAGACACCAGGCATCT
  CAATGGAGAGGAGGACGCCGGCGGGAGGGAAGACTCGATCCTCGTCAACGGGGCCTGCAG
  CGACCAGTCCTCCGACTCGCCCCCAATCCTGGAGGCTATCCGCACCCCGGAGATCAGAGG
  CCGAAGATCAAGCTCGCGACTCTCCAAGAGGGGAGGTGTCCAGTCTGCTAAGCTACACAC
  AGGACTTGACAGGCGATGGCGACGGGAAAGGGNNGGAATGGCTCTGACACCCCAGTCATG
  CCAAAGCTCTTCCGGGAAACCAGGACTCGTTCAGAAAGCCCAGCTTCCCTGAGACGGCGG
  GCAACAGCATCGGCAGGAACGCCATGGGCCGTCCCCTCCCAGCTCTTACCTTACCATCGA
  CCTCACAGACGACACAGAGGACACACATGGGACGCCCCAGAGCAGCAGTACCCCCTACGC
  CCGCCTAGCCCAGGACAGCCAGCAAGGGGGGCATGGAGCCCCCGCAGGTGGAGGCAGACA
  GTGGGAGATGAAAACAGTTCAAAGATCAGGATGGGAAAGGGATTTGGAATTAGGGGACCC
  TGTGTGGGGAAAAGAACAAGGGCTTCTCTTGGTGGCCCCCTGTTGGGGGTCTTGAAAAGC
  CCCTCCCAACCAAAGGCTTTGTTTGCTTTCCGGGGGTCCANNTGTTTTGGNNATGCAATT
  TTTCCAGGTTTTTNNAACAAATTGTGGCCTGGGGNTTTTTTACCCCCATTTTTTTTNGCA
  CTCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_175848 unedited
  TGGACCGCGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTAAACAA
  ATACTGATTTTAATTAAACATAAGGTAAACTCTAGGCATCCGTCATCTTTCAGCCTAAAA
  ATTAGCAAAAACTGTTGAAACAAGGCACAGTTTTTTCCCCATATTTGTTACGTCGTGGCT
  CCAGTTACAAAAAAATTTTAATGAAAACGTTAAACATAAAAATAGAAGTTTGAGATTTTA
  AAAAGTGTATAAAAAGCCCCACAAAACTTGTCAACGTTGTTCCTTATTCTACAAAATAGC
  ACCAGTAAGAAGAGTAAAAGGTGTTAAAAACCATTATGACAGCATTTCTGAAATGCAGCT
  TGTCTGAATTCCCGTTCTCCCTAAAAACGACTTCTTATGGAATAAAAAAGGATTAAAAAA
  TCTCCAAAGGGAGCACCGAGCTTTGCAGTTTTCCCTGTCATCTCTCAGATGTGGGGAAGG
  TATGAGAAATGTATGTCTGTCCCTGACTGCTGTCACTGCCTCTGAGTTTAGTAAAAAGAT
  GAGAAATGAGGGTAGCAGACTTCTCATCTGGGGACCTGTGCCTGTGGAGGGTAGGTCTCC
  TGGAAGGGAATGGGTTGAGGGGGGGTTTTATCTAAGCTCTTGCTTTCACTCCTTTCTAGG
  GAAGAGGAGATCCGGGGAGCCTTCCTGCCTTCAAAATATGGCGCGTTAATTTAGAACTGA
  GACACCCTTGCCTGACCCCCAGAGGCTCTTTTGTATTACGCGGAGGTTATACCAAAAACG
  CCCCTCTCCTTGACCTCGTGGGCAATCATTTTTTTTTTTCATTAACCCCTCATCGACAAA
  CTCATTAGTCCGTCCATCCCCCCGCCTCCCCCCTCTTTCCCTCCTCCCTACTTTCGCTCC
  CCCCCTCGTTTTCTCTTCCCCCACCTCTCCTTAGTCCCCCCCCATCCCCTCCTTCCTCTC
  CCCATTCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_175848
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175848.1, NP_787044.1
• RefSeq Size: 4293 bp
• RefSeq ORF: 2502 bp
• Locus ID: 1789
• Cytogenetics: 20q11.21
• Protein Families: Druggable Genome, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways
• Gene Summary: 'CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq, May 2011]'
  Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. Isoform 2 is shorter than isoform 1.