TAZ (NM_181313) Human Untagged Clone

CAT#: SC125060

TAZ (untagged)-Human tafazzin (TAZ), nuclear gene encoding mitochondrial protein, transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TAZ
• Synonyms: BTHS; CMD3A; EFE; EFE2; G4.5; LVNCX; Taz1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125060 sequence for NM_181313 edited (data generated by NextGen Sequencing)
  ATGCCTCTGCACGTGAAGTGGCCGTTCCCCGCGGTGCCGCCGCTCACCTGGACCCTGGCC
  AGCAGCGTCGTCATGGGCTTGGTGGGCACCTACAGCTGCTTCTGGACCAAGTACATGAAC
  CACCTGACCGTGCACAACAGGGAGGTGCTGTACGAGCTCATCGAGAAGCGAGGCCCGGCC
  ACGCCCCTCATCACCGTGTCCAATCACCAGTCCTGCATGGACGACCCTCATCTCTGGGGG
  ATCCTGAAACTCCGCCACATCTGGAACCTGAAGTTGATGCGTTGGACCCCTGCAGCTGCA
  GACATCTGCTTCACCAAGGAGCTACACTCCCACTTCTTCAGCTTGGGCAAGTGTGTGCCT
  GTGTGCCGAGGAGATGGCGTCTACCAGAAGGGGATGGACTTCATTTTGGAGAAGCTCAAC
  CATGGGGACTGGGTGCATATCTTCCCAGAAGGAATCGGGCGCCTGATTGCTGAGTGTCAT
  CTCAACCCCATCATCCTGCCCCTGTGGCATGTCGGAATGAATGACGTCCTTCCTAACAGT
  CCGCCCTACTTCCCCCGCTTTGGACAGAAAATCACTGTGCTGATCGGGAAGCCCTTCAGT
  GCCCTGCCTGTACTCGAGCGGCTCCGGGCGGAGAACAAGTCGGCTGTGGAGATGCGGAAA
  GCCCTGACGGACTTCATTCAAGAGGAATTCCAGCATCTGAAGACTCAGGCAGAGCAGCTC
  CACAACCACCTCCAGCCTGGGAGATAG
  
  Clone variation with respect to NM_181313.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_181313 unedited
  GTTAGCATTTGTATACGACTTTACTATAGGGCGGCCGCGAATTCGCACCAGCCGGCCCGG
  GGCTCTGGGAGCGCCGGCCGCGGGCCGGGTGGGGATGCCTCTGCACGTGAAGTGGCCGTT
  CCCCGCGGTGCCGCCGCTCACCTGGACCCTGGCCAGCAGCGTCGTCATGGGCTTGGTGGG
  CACCTACAGCTGCTTCTGGACCAAGTACATGAACCACCTGACCGTGCACAACAGGGAGGT
  GCTGTACGAGCTCATCGAGAAGCGAGGCCCGGCCACGCCCCTCATCACCGTGTCCAATCA
  CCAGTCCTGCATGGACGACCCTCATCTCTGGGGGATCCTGAAACTCCGCCACATCTGGAA
  CCTGAAGTTGATGCGTTGGACCCCTGCAGCTGCAGACATCTGCTTCACCAAGGAGCTACA
  CTCCCACTTCTTCAGCTTGGGCAAGTGTGTGCCTGTGTGCCGAGGAGATGGCGTCTACCA
  GAAGGGGATGGACTTCATTNTGGAGAAGCTCAACCATGGGGACTGGGTGCATATCTTCCC
  AGAAGGGAAAGTGAACATGAGTTCCGAATTCCTGCGTTTCAAGTGGGGAATCGGGCGCCT
  GATTGCTGAGTGTCATCTCANACCCCATCATCCTGCCCTGTGGCATGTCNGAATGAATGA
  CGTCCTTCCTAACAGTCCGNCCTACTTTCCCCGCTTTGGACAGAAAATCACTGTGCTGAT
  CGGGAAGCCCTTCAGTGCCCTGCCTGTACTCGAGCGGCTCCGGNNCGAGACCAGTCGGCT
  GTTGAGATGCCGAAAGCCTGACGGACTTCATTCAGAAGAATTCCACCTCTGGAGACTTAG
  CAGACAGCTTCACACCACTCCAGCTGGAGATAGCCTGCTGCTGCTTCTGATTCTGGCCGC
  CANACTGNGCTGAGGATGACTGAGCTTTACTCAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_181313
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181313.1, NP_851830.1
• RefSeq Size: 1772 bp
• RefSeq ORF: 747 bp
• Locus ID: 6901
• Cytogenetics: Xq28
• Protein Families: ES Cell Differentiation/IPS, Transmembrane
• Gene Summary: 'This gene encodes a protein that is expressed at high levels in cardiac and skeletal muscle. Mutations in this gene have been associated with a number of clinical disorders including Barth syndrome, dilated cardiomyopathy (DCM), hypertrophic DCM, endocardial fibroelastosis, and left ventricular noncompaction (LVNC). Multiple transcript variants encoding different isoforms have been described. A long form and a short form of each of these isoforms is produced; the short form lacks a hydrophobic leader sequence and may exist as a cytoplasmic protein rather than being membrane-bound. Other alternatively spliced transcripts have been described but the full-length nature of all these transcripts is not known. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (4) lacks two in-frame coding exons compared to variant 1, resulting in a shorter isoform (4) compared to isoform 1.