EAAT4 (SLC1A6) (NM_005071) Human Untagged Clone

CAT#: SC125329

SLC1A6 (untagged)-Human solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6 (SLC1A6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC1A6
• Synonyms: EAAT4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005071 edited
  GCACGAGCAGGGCCGGCCCGGATCCCTCCCGCAGCATAGCGCGTCCCGGCTCCGCGCCGG
  TGCCTCCACGGTCCGGTCCCCGCGCCGGTGCTGCACAGTCCCTGGCGGGTCCCCGCGGCC
  CCGGCCGGGCGCTTCGCCGGGCTCCGGCTCCTGCATCCGGGCGCAGCGCGCAGGCCGAGG
  CGCGGGCAGGCCGCCCCCGCCGCTCCGGACGCCGACCATGAGCAGCCATGGCAACAGCCT
  GTTCCTTCGGGAGAGCGGCCAGCGGCTGGGCCGGGTGGGCTGGCTGCAGCGGCTGCAGGA
  AAGCCTGCAGCAGAGAGCACTGCGCACGCGCCTGCGCCTGCAGACCATGACCCTCGAGCA
  CGTGCTGCGCTTCCTGCGCCGAAACGCCTTCATTCTGCTGACGGTCAGCGCCGTGGTCAT
  TGGGGTCAGCCTGGCCTTTGCCCTGCGCCCATATCAGCTCACCTACCGCCAGATCAAGTA
  CTTCTCTTTTCCTGGAGAGCTTCTGATGAGGATGCTGCAGATGCTGGTGTTACCTCTCAT
  TGTCTCCAGCCTGGTCACAGGTATGGCATCCCTGGACAACAAGGCCACGGGGCGGATGGG
  GATGCGGGCAGCTGTGTACTACATGGTGACCACCATCATCGCGGTCTTCATCGGCATCCT
  CATGGTCACCATCATCCATCCCGGGAAGGGCTCCAAGGAGGGGCTGCACCGGGAGGGCCG
  GATCGAGACCATCCCCACAGCTGATGCCTTCATGGACCTGATCAGAAATATGTTTCCACC
  AAACCTTGTGGAGGCCTGCTTCAAACAGTTCAAGACGCAGTACAGCACGAGGGTGGTAAC
  CAGGACCATGGTGAGGACAGAGAACGGGTCTGAGCCGGGTGCCTCCATGCCTCCTCCATT
  CTCAGTGGAGAACGGAACCAGCTTCCTGGAAAATGTCACTCGGGCCTTGGGTACCCTGCA
  GGAGATGCTGAGCTTTGAGGAGACTGTACCCGTGCCTGGCTCCGCCAATGGCATCAACGC
  CCTGGGCCTCGTGGTCTTCTCTGTGGCCTTTGGGCTGGTCATTGGTGGCATGAAACACAA
  GGGCAGAGTCCTCAGGGACTTCTTCGACAGCCTCAATGAGGCTATTATGAGGCTGGTGGG
  CATCATTATCTGGTATGCACCTGTGGGCATCCTGTTCCTGATTGCTGGGAAGATTCTGGA
  GATGGAAGACATGGCCGTCCTGGGGGGTCAGCTGGGCATGTACACCCTGACCGTCATCGT
  GGGCCTGTTCCTCCATGCCGGCATTGTCCTTCCCCTCATCTACTTCCTCGTCACTCACCG
  GAACCCCTTCCCCTTCATTGGGGGCATGCTACAAGCCCTCATCACCGCTATGGGCACGTC
  TTCCAGCTCGGCAACGCTGCCCATCACCTTCCGCTGCCTGGAGGAGGGCCTGGGTGTGGA
  CCGCCGCATCACCAGGTTCGTCCTGCCCGTGGGCGCCACGGTCAACATGGATGGCACTGC
  CCTCTACGAGGCCCTGGCTGCCATCTTCATTGCTCAAGTTAACAACTACGAGCTCAACCT
  GGGTCAGATCACAACCATCAGCATCACGGCCACAGCAGCCAGTGTTGGGGCTGCTGGCAT
  CCCCCAGGCGGGTCTGGTCACCATGGTCATTGTGCTTACGTCGGTCGGCTTGCCCACGGA
  AGACATCACGCTCATCATTGCCGTGGACTGGTTCCTTGACCGGCTTCGCACAATGACCAA
  CGTACTGGGGGACTCAATTGGAGCGGCCGTCATCGAGCACTTGTCTCAGCGGGAGCTGGA
  GCTTCAGGAAGCTGAGCTTACCCTCCCCAGCCTGGGGAAACCCTACAAGTCCCTCATGGC
  ACAGGAGAAGGGGGCATCCCGGGGACGGGGAGGCAACGAGAGTGCTATGTGAGGGGCCTC
  CAGCTCTGCCCCCCCAGAGAGGAGGGAAGGGGGCTGGGGAGGGGAGTCCTGGTGACACAT
  CTGTTGCCCAACTGACCGTGGGCTGAACACACGTTCTGCTTGACTCATTTAGGGGGGAGG
  GAAAAGTGAAATAAAGGAGCAGGAATGAAAGGTAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005071 unedited
  NNCGTCACCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGCAGGGCCG
  GCCCGGATCCCTCCCGCAGCATAGCGCGTCCCGGCTCCGCGCCGGTGCCTCCACGGTCCG
  GTCCCCGCGCCGGTGCTGCACAGTCCCTGGCGGGTCCCCGCGGCCCCGGCCGGGCGCTTC
  GCCGGGCTCCGGCTCCTGCATCCGGGCGCAGCGCGCAGGCCGAGGCGCGGGCAGGCCGCC
  CCCGCCGCTCCGGACGCCGACCATGAGCAGCCATGGCAACAGCCTGTTCCTTCGGGAGAG
  CGGCCAGCGGCTGGGCCGGGTGGGCTGGCTGCAGCGGCTGCAGGAAAGCCTGCAGCAGAG
  AGCACTGCGCACGCGCCTGCGCCTGCAGACCATGACCCTCGAGCACGTGCTGCGCTTCCT
  GCGCCGAAACGCCTTCATTCTGCTGACGGTCAGCGCCGTGGTCATTGGGGTCAGCCTGGC
  CTTTGCCCTGCGCCCATATCAGCTCACCTACCGCCAGATCAAGTACTTCTCTTTTCCTGG
  AGAGCTTCTGATGAGGATGCTGCAGATGCTGGTGTTACCTCTCATTGTCTCCAGCCTGGT
  CACAGGTATGGCATCCCTGGACAACAAGGCCACGGNGCGGATGGGGATGCNGGCAGCTGT
  GTACTACATGGTGACCACCATCATCGCGGGTCTCATCGGCATCCTCATGGTCACCATCAT
  CCATCCCGGGAAGGGGCTCCAGGANGGGCTGCACCGGNAGGGCCGNATCGAGACCATCCC
  ACAGCTGATGCCTTCATGGACCTGATCAGAAATATGTTNCACCANACCTTGTNAGGCNCC
  TGCTCAACAGTCAAGACGCATCAGCACGAGGGTGGTAACCAGACATGGTGAGACANANNA
  CGGTCTGAGCCGGNTGCCTCATGCCTCTCATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005071
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005071.1, NP_005062.1
• RefSeq Size: 1719 bp
• RefSeq ORF: 1695 bp
• Locus ID: 6511
• Cytogenetics: 19p13.12
• Domains: SDF
• Protein Families: Transmembrane
• Gene Summary: ''
  Transcript Variant: This variant (2) differs in the 5' UTR and its transcription extends past a splice site that is used in variant 1, resulting in a novel 3' coding region and 3' UTR compared to variant 1. It encodes isoform 2 which is longer and has a distinct C-terminus, compared to isoform 1.