SEPTIN4 (NM_080416) Human Untagged Clone

CAT#: SC125356

41521 (untagged)-Human septin 4 (SEPT4), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SEPTIN4
• Synonyms: ARTS; BRADEION; C17orf47; CE5B3; H5; hCDCREL-2; hucep-7; MART; PNUTL2; SEP4; SEPT4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125356 sequence for NM_080416 edited (data generated by NextGen Sequencing)
  ATGATCAAGCGTTTCCTGGAGGACACCACGGATGATGGAGAACTGAGCAAGTTCGTGAAG
  GATTTCTCAGGAAATGCGAGCTGCCACCCACCAGAGGCTAAGACCTGGGCATCCAGGCCC
  CAAGTCCCGGAGCCAAGGCCCCAGGCCCCGGACCTCTATGATGATGACCTGGAGTTCAGA
  CCCCCCTCGCGGCCCCAGTCCTCTGACAACCAGCAGTACTTCTGTGCCCCAGCCCCTCTC
  AGCCCATCTGCCAGGCCCCGCAGCCCATGGGGCAAGCTTGATCCCTATGATTCCTCTGAG
  GATGACAAGGAGTATGTGGGCTTTGCAACCCTCCCCAACCAAGTCCACCGAAAGTCCGTG
  AAGAAAGGCTTTGACTTTACCCTCATGGTGGCAGGAGAGTCTGGCCTGGGCAAATCCACA
  CTTGTCAATAGCCTCTTCCTCACTGATCTGTACCGGGACCGGAAACTTCTTGGTGCTGAA
  GAGAGGATCATGCAAACTGTGGAGATCACTAAGCATGCAGTGGACATAGAAGAGAAGGGT
  GTGAGGCTGCGGCTCACCATTGTGGACACACCAGGTTTTGGGGATGCAGTCAACAACACA
  GAGTGCTGGAAGCCTGTGGCAGAATACATTGATCAGCAGTTTGAGCAGTATTTCCGAGAC
  GAGAGTGGCCTGAACCGAAAGAACATCCAAGACAACAGGGTGCACTGCTGCCTGTACTTC
  ATCTCACCCTTCGGCCATGGGCTCCGGCCATTGGATGTTGAATTCATGAAGGCCCTGCAT
  CAGCGGGTCAACATCGTGCCTATCCTGGCTAAGGCAGACACACTGACACCTCCCGAAGTG
  GACCACAAGAAACGCAAAATCCGGGAGGAGATTGAGCATTTTGGAATCAAGATCTATCAA
  TTCCCAGACTGTGACTCTGATGAGGATGAGGACTTCAAATTGCAGGACCAAGCCCTAAAG
  GAAAGCATCCCATTTGCAGTAATTGGCAGCAACACTGTAGTAGAGGCCAGAGGGCGGCGA
  GTTCGGGGTCGACTCTACCCCTGGGGCATCGTGGAAGTGGAAAACCCAGGGCACTGCGAC
  TTTGTGAAGCTGAGGACAATGCTGGTACGTACCCACATGCAGGACCTGAAGGATGTGACA
  CGGGAGACACATTATGAGAACTACCGGGCACAGTGCATCCAGAGCATGACCCGCCTGGTG
  GTGAAGGAACGGAATCGCAACAAACTGACTCGGGAAAGTGGTACCGACTTCCCCATCCCT
  GCTGTCCCACCAGGGACAGATCCAGAAACTGAGAAGCTTATCCGAGAGAAAGATGAGGAG
  CTGCGGCGGATGCAGGAGATGCTACACAAAATACAAAAACAGATGAAGGAGAACTATTAA
  
  Clone variation with respect to NM_080416.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_080416 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCTGCAGCCATGATCAAGC
  GTTTCCTGGAGGACACCACGGATGATGGAGAACTGAGCAAGTTCGTGAAGGATTTCTCAG
  GAAATGCGAGCTGCCACCCACCAGAGGCTAAGACCTGGGCATCCAGGCCCCAAGTCCCGG
  AGCCAAGGCCCCAGGCCCCGGACCTCTATGATGATGACCTGGAGTTCAGACCCCCCTCGC
  GGCCCCAGTCCTCTGACAACCAGCAGTACTTCTGTGCCCCAGCCCCTCTCAGCCCATCTG
  CCAGGCCCCGCAGCCCATGGGGCAAGCTTGATCCCTATGATTCCTCTGAGGATGACAAGG
  AGTATGTGGGCTTTGCAACCCTCCCCAACCAAGTCCACCGAAAGTCCGTGAAGAAAGGCT
  TTGACTTTACCCTCATGGTGGCAGGAGAGTCTGGCCTGNGCAAATCCACACTTGTCAATA
  GCCTCTTCCTCACTGATCTGTACCGGGACCGGAAACTTCTTGGTGCTGAAGAGAGGATCA
  TGCAAACTGTGGAGATCACTAAGCATGCAGTGGACATAGAAGAGAAGGTGTGATGCTGCG
  GTTCACCTTGTGGACACCCCAGGTTTTGGGGATGCACCCACAACACAGACGGCTGGAGCC
  TGTGGCAGATACATTGATCAGAGCTTGACCATTTTTCAAGACAGAGGGGCTGACCCGAAA
  AACTCCAAGACACAGGGTGCCCGGCTGCTGTACTTATCTCACCCTCGGCCCGGCCCTCCG
  GCCTTGGATGTTGAATCATGAAGGCCGCATAAACGGCCAACTCGGGCCTTCCTGCTAAGA
  GGACACTGACCCCTCCGAGGGGCCCAAGAAACCCATCCGGGGGGATTGCCCTTTGAATCA
  AACCTCCCCCCACCTGGGCCTCGAGAGAAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_080416
• Insert Size: 1900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080416.1, NP_536341.1
• RefSeq Size: 1669 bp
• RefSeq ORF: 1380 bp
• Locus ID: 5414
• Cytogenetics: 17q22
• Domains: GTP_CDC
• Gene Summary: 'This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer. [provided by RefSeq, Nov 2010]'
  Transcript Variant: This variant (3) differs in the 5' UTR and coding region and initiates translation at an alternate start codon, compared to variant 7. It encodes isoform 3 (also known as PNUTL2b, hcdcrel2b) with a distinct N-terminus compared to isoform 5.