alpha Adducin (ADD1) (NM_176801) Human Untagged Clone

CAT#: SC125367

ADD1 (untagged)-Human adducin 1 (alpha) (ADD1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADD1
• Synonyms: ADDA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_176801, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAATGGTGATTCTCGTGCTGCGGTGGTGACCTCACCACCCCCGACCACAGCCCCTCACAAGGAGAGGT
  ACTTCGACCGAGTAGATGAGAACAACCCAGAGTACTTGAGGGAGAGGAACATGGCACCAGACCTTCGCCA
  GGACTTCAACATGATGGAGCAAAAGAAGAGGGTGTCCATGATTCTGCAAAGCCCTGCTTTCTGTGAAGAA
  TTGGAATCAATGATACAGGAGCAATTTAAGAAGGGGAAGAACCCCACAGGCCTATTGGCATTACAGCAGA
  TTGCAGATTTTATGACCACGAATGTACCAAATGTCTACCCAGCAGCTCCGCAAGGAGGGATGGCTGCCTT
  AAACATGAGTCTTGGTATGGTGACTCCTGTGAACGATCTTAGAGGATCTGATTCTATTGCGTATGACAAA
  GGAGAGAAGTTATTACGGTGTAAATTGGCAGCGTTTTATAGACTAGCAGATCTCTTTGGGTGGTCTCAGC
  TTATCTACAATCATATCACAACCAGAGTGAACTCCGAGCAGGAACACTTCCTCATTGTCCCTTTTGGGCT
  TCTTTACAGTGAAGTGACTGCATCCAGTTTGGTTAAGATCAATCTACAAGGAGATATAGTAGATCGTGGA
  AGCACTAATCTGGGAGTGAATCAGGCCGGCTTCACCTTACACTCTGCAATTTATGCTGCACGCCCGGACG
  TGAAGTGCGTCGTGCACATTCACACCCCAGCAGGGGCTGCGGTCTCTGCAATGAAATGTGGCCTCTTGCC
  AATCTCCCCGGAGGCGCTTTCCCTTGGAGAAGTGGCTTATCATGACTACCATGGCATTCTGGTTGATGAA
  GAGGAAAAAGTTTTGATTCAGAAAAATCTGGGGCCTAAAAGCAAGGTTCTTATTCTCCGGAACCATGGGC
  TCGTGTCAGTTGGAGAGAGCGTTGAGGAGGCCTTCTATTACATCCATAACCTTGTGGTTGCCTGTGAGAT
  CCAGGTTCGAACTCTGGCCAGTGCAGGAGGACCAGACAACTTAGTCCTGCTGAATCCTGAGAAGTACAAA
  GCCAAGTCCCGTTCCCCAGGGTCTCCGGTAGGGGAAGGCACTGGATCGCCTCCCAAGTGGCAGATTGGTG
  AGCAGGAATTTGAAGCCCTCATGCGGATGCTCGATAATCTGGGCTACAGAACTGGCTACCCTTATCGATA
  CCCTGCTCTGAGAGAGAAGTCTAAAAAATACAGCGATGTGGAGGTTCCTGCTAGTGTCACAGGTTACTCC
  TTTGCTAGTGACGGTGATTCGGGCACTTGCTCCCCACTCAGACACAGTTTTCAGAAGCAGCAGCGGGAGA
  AGACAAGATGGCTGAACTCTGGCCGGGGCGACGAAGCTTCCGAGGAAGGGCAGAATGGAAGCAGTCCCAA
  GTCGAAGACTAAGGTGTGGACGAACATTACACACGATCACGTGAAACCCTTGCTGCAGTCTCTCTCGTCC
  GGTGTCTGCGTGCCAAGCTGTATTACCAACTGCTTGTGGACTAAAGAGGATGGACATAGAACTTCCACCT
  CTGCTGTCCCTAACCTGTTTGTTCCATTGAACACTAACCCAAAAGAGGTCCAGGAGATGAGGAACAAGAT
  CCGAGAGCAGAATTTACAGGACATTAAGACGGCTGGCCCTCAGTCCCAGGTTTTGTGTGGTGTAGTGATG
  GACAGGAGCCTCGTCCAGGGAGAGCTGGTGACGGCCTCCAAGGCCATCATTGAAAAGGAGTACCAGCCCC
  ACGTCATTGTGAGCACCACGGGCCCCAACCCCTTCACCACACTCACAGACCGTGAGCTGGAGGAGTACCG
  CAGGGAGGTGGAGAGGAAGCAGAAGGGCTCTGAAGAGAATCTGGACGAGGCTAGAGAACAGAAAGAAAAG
  AGTCCTCCAGACCAGCCTGCGGTCCCCCACCCGCCTCCCAGCACTCCCATCAAGCTGGAGGAAGGAGACG
  GATGCGCTAGAGAGTACCTGTTACCCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_176801 unedited
  TTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGAGAGGCCTGGCGGGCC
  GCTGCTGCGGGCCAGGGGACGGGGGCGGAGCCGGAGCCGGAGCCGACGGGCGGTGGCCGC
  ACTGGGACCCCGGAATCCCGCGCGCTGCCCACGATTCGCTTCTGAGGAACCTAGAAAGAT
  TGTACAATGAATGGTGATTCTCGTGCTGCGGTGGTGACCTCACCACCCCCGACCACAGCC
  CCTCACAAGGAGAGGTACTTCGACCGAGTAGATGAGAACAACCCAGAGTACTTGAGGGAG
  AGGAACATGGCACCAGACCTTCGCCAGGACTTCAACATGATGGAGCAAAAGAAGAGGGTG
  TCCATGATTCTGCAAAGCCCTGCTTTCTGTGAAGAATTGGAATCAATGATACAGGAGCAA
  TTTAAGAAGGGGAAGAACCCCACAGGCCTATTGGCATTACAGCAGATTGCAGATTTTATG
  ACCACGAATGTACCAAATGTCTACCCAGCAGCTCCGCAAGGAGGGATGGCTGCCTTAAAC
  ATGAGTCTTGGTATGGTGACTCCTGTGAACGATCTTAGAGGATCTGATTCTATTGCGTAT
  GACAAAGGAGAGAAGTTATTACGGTGTAAATTGGCAGCGTTTTATAGACTAGCAGATCTC
  TTTGGGTGGTCTCAGCTTATCTACAATCATATCACAACCAGAGTGAACTCCGAGCAGGAA
  CACTTCCTCATTGTCCCTTTTGGGCTTCTTTACAGTGAAGTGACTGCATNCAGTTTGGTT
  AAGATCAATCTACANGGAGATATAGTAGATCGTGGAAGCACTAATCTGGGAGTGAAATCA
  GCCGGCTTCACCTTNACACTCTGCATTATGCTGCACCCCCGGACGTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_176801 unedited
  CCGGGATTGTCGATTTTTTATTGTGNAGTAAAACTGTTACACTGGNANAAGGNCATTCAG
  GACCTGTCCTCCCAGTCGGTGTCCATATATGCAACTATATTTCATGTTGTAATACAAAGG
  ATTAAACTACAAAAGTGAAAATTAAAAGTCATTGCATATGTGGGGTAAAGGAGGGGATAA
  AGGAAGAGTCCACGGTTAAGGACTTTTCATTCTGCCTTGGCAACAAAACGACACAGAAGA
  GAGGCAGCACGCTTGCCCGCGTAGCAGGCCCTGCGGCTCAGAGGAAGCTGGAAGCGGCGG
  TCAGTGCCATGCAGCAGCCTGGTTCTCGCTCGGCTGGCCAATCAGTGGGATCCGAGGTGG
  CTCGGCCCATCATGCCCAGGATCTGAGCCCACATCTGGCACCAGCTCTACCAAGGGCCTG
  TTGGGCCCACACAAGCTTGCCTGTACCTAGTCAGGCTGGTACCGGCACCTCCTGAAATGG
  GTCACCCTCCGGTTCCTGCTCAGCAGCTCTGAGCCCTTTTCCAGCCTCTTGGAGGGAAGT
  AACAACAACAGCATCTGCCGAGGAGCACACGGTTCAGGCTCCATCAATGACGGTGATGTC
  GAGGGGCCCCAGGGCTACCCTCCACCTCGAGTTACCTTTGATCACAACTAGGGCATCTTT
  CTGGCTGAGTTAAGGTGAGAACAGTCCCCTGAAGCCTCCCCCCAATAAGTGAAAAGTGCT
  CTACTGTGGGGGTCTGGAAAGGAGAAAGCAAAAAGCAGCCTATTCACTTTCACAGTTCGA
  AAATGTAAGCTAAATTGTAAACCTGCTCAAAGAAAATTCTAGAAAGGCCTCTTTCCCTCC
  TGATTACCCTCCCTATGACAGGGATTCAGAAACCCCGTGAAAAAGGAACCGAGCTCCTGG
  ATCTGTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_176801
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176801.1, NP_789771.1
• RefSeq Size: 4068 bp
• RefSeq ORF: 1989 bp
• Locus ID: 118
• Cytogenetics: 4p16.3
• Protein Families: Druggable Genome
• Gene Summary: 'Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, and gamma). Adducin acts as a heterodimer of the related alpha, beta, or gamma subunits. The protein encoded by this gene represents the alpha subunit. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. [provided by RefSeq, Aug 2017]'
  Transcript Variant: This variant (4) utilizes an alternate splice site and includes an additional exon in the coding region, which results in a frameshift, compared to variant 2. The encoded protein (isoform d) contains an early stop codon and shorter C-terminus, compared to isoform b.