Deltex (DTX1) (NM_004416) Human Untagged Clone

CAT#: SC125438

DTX1 (untagged)-Human deltex homolog 1 (Drosophila) (DTX1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DTX1
• Synonyms: hDx-1; RNF140
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125438 sequence for NM_004416 edited (data generated by NextGen Sequencing)
  ATGTCACGGCCAGGCCACGGTGGGCTGATGCCTGTGAATGGTCTGGGCTTCCCACCGCAG
  AACGTGGCCCGGGTGGTGGTGTGGGAGTGGCTGAATGAGCACAGCCGCTGGCGGCCCTAC
  ACGGCCACCGTGTGCCACCACATTGAGAACGTGCTGAAGGAGGACGCTCGCGGTTCCGTG
  GTCCTGGGGCAGGTGGACGCCCAGCTTGTGCCCTACATCATCGACCTGCAGTCCATGCAC
  CAGTTTCGCCAGGACACAGGCACCATGCGGCCCGTGCGGCGCAACTTCTACGACCCGTCG
  TCGGCGCCGGGCAAGGGCATCGTGTGGGAGTGGGAGAACGACGGCGGCGCATGGACGGCC
  TACGATATGGACATCTGCATCACCATCCAGAACGCCTACGAGAAGCAGCACCCGTGGCTC
  GACCTCTCATCGCTAGGCTTCTGCTACCTCATCTACTTCAACAGCATGTCGCAGATGAAC
  CGCCAGACGCGCCGGCGCCGCCGCCTGCGCCGCCGCCTGGACCTCGCCTACCCGCTCACC
  GTGGGCTCCATCCCTAAGTCGCAGTCGTGGCCCGTGGGCGCCAGCTCGGGCCAGCCCTGC
  TCCTGCCAGCAGTGCCTGCTGGTCAACAGCACGCGCGCCGCCTCCAACGCCATCCTGGCC
  TCGCAGCGCCGCAAGGCGCCCCCCGCGCCCCCGCTGCCGCCGCCGCCGCCACCTGGAGGG
  CCTCCAGGCGCGCTTGCCGTGCGCCCCAGCGCCACCTTCACAGGCGCCGCGCTCTGGGCA
  GCGCCCGCCGCCGGCCCCGCCGAGCCCGCGCCGCCTCCCGGGGCGCCCCCACGGAGCCCG
  GGCGCCCCCGGCGGAGCGCGCACCCCGGGGCAGAACAACCTCAACCGGCCCGGGCCCCAG
  CGCACCACCAGCGTGAGCGCGCGCGCCTCCATCCCGCCGGGGGTCCCCGCACTCCCGGTG
  AAGAACTTGAATGGTACTGGGCCGGTCCATCCGGCCCTGGCAGGGATGACCGGGATACTG
  CTGTGCGCGGCCGGGCTGCCCGTGTGCCTGACGCGGGCCCCCAAGCCCATCCTGCACCCG
  CCGCCCGTGAGCAAGAGCGACGTGAAGCCCGTGCCTGGCGTGCCCGGGGTGTGCCGCAAG
  ACCAAGAAGAAGCACCTTAAAAAGAGTAAGAATCCCGAGGATGTGGTTCGAAGATACATG
  CAGAAGGTGAAAAACCCACCTGATGAGGACTGTACCATCTGCATGGAGCGGCTGGTCACA
  GCATCAGGCTACGAGGGCGTGCTTCGGCACAAGGGCGTGCGGCCTGAGCTCGTGGGCCGC
  CTGGGCCGCTGTGGCCACATGTACCACCTGCTGTGCCTCGTGGCCATGTACTCCAATGGC
  AACAAGGATGGCAGCCTGCAGTGCCCCACCTGCAAGGCCATCTATGGGGAGAAGACGGGT
  ACGCAGCCGCCTGGGAAGATGGAGTTCCACCTCATCCCCCACTCGCTGCCCGGCTTCCCT
  GATACCCAGACCATCCGCATCGTCTATGACATCCCCACAGGCATCCAGGGCCCTGAGCAC
  CCCAACCCCGGGAAGAAGTTCACCGCAAGAGGATTCCCTCGCCACTGCTATCTACCCAAC
  AACGAGAAAGGCCGGAAGGTGCTGCGGCTGCTCATCACGGCCTGGGAGAGAAGACTCATC
  TTCACTATCGGCACGTCCAACACCACGGGCGAGTCGGACACCGTGGTGTGGAACGAGATC
  CACCACAAGACCGAGTTTGGATCCAACCTCACGGGCCACGGCTACCCGGACGCTAGCTAC
  CTAGACAACGTGCTGGCTGAGCTCACAGCCCAGGGCGTATCCGAGGCTGCAGCCAAGGCT
  TGA
  
  Clone variation with respect to NM_004416.2
  1233 c=>t;1251 a=>g;1425 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004416 unedited
  ACGTTTGNAACCCACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGAGCTGTTTCC
  TCCGGCATAAGAGAGACACTTGCTTTCCAGGGCAGCACCCTTTATCGGAGAAGGCTCTAC
  AGGGAAGGGGTCTTTGCAGCCTGGATGGCCATCCCACATTCCTTTAACGGAGGTCTCTAG
  GCCTCAGAGAGAACCCAGAGTTAGAAAGGAGGCCAGACGGTCCTTGCTGTCCCCCTGGGG
  AGAGAGGAAGTTGCCGCCTGCTGCCAGGCCCAGGAGGAGCTGGGCCTGCAATAGTGGGGG
  ACCTGGCCCCTGAGGCAGTGGCGGCCATGTCACGGCCAGGCCACGGTGGGCTGATGCCTG
  TGAATGGTCTGGGCTTCCCACCGCAGAACGTGGCCCGGGTGGTGGTGTGGGAGTGACTGA
  ATGAACACAGTCGCTCTGCGCCCGTACCACGCGCACGTGGTGCCTACCCACATTGGGAAC
  CCTTCCAAACAGAAGAAACCCTCCCGGGCTCCCCTGGCTGTCCTGGCGCGATAGCTCGCA
  ACGCCCACTCCGTGGCCCCCAACAACAGAGATCGTAGCAAGCCTCTGAACACCCCATACA
  CATATTACCATTCCCCAAACATCTCGCGTGCACACCGCCCTCTCCCATTGCTAGCCCTAA
  CACTTTCATCGTATACTAACAGTGCGTGTACCGTCACGCTACGTCTCAATACGCACTTCC
  CGCATCGCTGCGGACGATGTGAGTGAGTCGCACGACATCTAAACTCACGCTCGCGTAAGC
  CGCGATTCTCATTAGAACTCAACTATCCGTCGACCTACTCGCCGTCGAGCGCAGAGACG
  
• Restriction Sites: Please inquire
• ACCN: NM_004416
• Insert Size: 3200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004416.2, NP_004407.2
• RefSeq Size: 3317 bp
• RefSeq ORF: 1863 bp
• Locus ID: 1840
• Cytogenetics: 12q24.13
• Domains: RING, WWE
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Notch signaling pathway
• Gene Summary: 'Studies in Drosophila have identified this gene as encoding a positive regulator of the Notch-signaling pathway. The human gene encodes a protein of unknown function; however, it may play a role in basic helix-loop-helix transcription factor activity. [provided by RefSeq, Jul 2008]'