Chimaerin 2 (CHN2) (NM_004067) Human Untagged Clone

CAT#: SC125452

CHN2 (untagged)-Human chimerin (chimaerin) 2 (CHN2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHN2
• Synonyms: ARHGAP3; BCH; CHN2-3; RHOGAP3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004067 edited
  ATGGCAGCGTCCAGCAACTCCAGCCTGTCCGGCTCGTCGGTGTCCTCCGATGCTGAAGAA
  TACCAGCCTCCTATATGGAAATCATACTTATATCAGTTACAGCAAGAGGCACCTCGTCCC
  AAGAGAATCATTTGTCCTCGGGAGGTGGAAAACAGACCAAAATATTATGGAAGAGAGTTT
  CATGGGATCATCTCTCGGGAGCAGGCGGATGAGCTTCTTGGAGGCGTGGAGGGTGCCTAC
  ATCCTTAGAGAAAGCCAGCGGCAACCAGGATGCTACACGCTGGCTCTCAGGTTTGGAAAC
  CAGACCTTAAACTACAGGCTCTTCCACGACGGGAAACACTTTGTGGGTGAGAAGAGGTTT
  GAGTCGATTCATGATCTGGTGACAGATGGCTTGATAACACTGTACATAGAAACAAAAGCT
  GCCGAGTACATTTCAAAAATGACAACTAACCCCATCTATGAACACATTGGATATGCCACC
  CTACTCAGAGAAAAAGTATCCAGAAGGCTGAGCAGGTCTAAAAATGAACCAAGAAAAACA
  AACGTCACACATGAAGAACACACAGCGGTGGAAAAGATCTCCTCCCTGGTTCGAAGGGCT
  GCCCTCACACACAACGACAACCACTTCAATTATGAGAAGACACACAACTTTAAGGTCCAC
  ACGTTCCGAGGCCCACACTGGTGTGAATATTGTGCCAATTTCATGTGGGGGCTCATCGCC
  CAAGGGGTCCGGTGCTCAGACTGTGGATTGAACGTACACAAACAGTGTTCCAAGCACGTT
  CCCAATGACTGCCAACCTGATCTCAAGAGGATCAAGAAAGTGTACTGTTGTGACCTCACA
  ACACTTGTGAAGGCTCACAACACTCAGAGACCCATGGTGGTAGACATATGCATTCGGGAA
  ATTGAAGCAAGAGGATTAAAATCGGAAGGCCTTTACAGAGTCTCTGGGTTCACTGAACAC
  ATTGAAGATGTCAAAATGGCATTTGACAGAGATGGTGAAAAGGCCGATATATCTGCCAAT
  GTCTATCCAGACATAAACATCATCACTGGAGCCCTTAAACTGTATTTCAGAGACTTACCC
  ATCCCTGTCATCACATATGATACCTATTCCAAATTTATAGATGCAGCAAAAATCTCCAAT
  GCAGATGAGAGGCTGGAAGCCGTCCATGAAGTGCTGATGCTGCTGCCTCCTGCCCACTAT
  GAAACCCTCCGGTACCTAATGATCCACCTCAAAAAGGTTACTATGAATGAAAAAGACAAT
  TTCATGAATGCAGAAAATCTGGGGATCGTGTTTGGGCCCACTCTGATGAGGCCCCCTGAG
  GACAGCACCCTGACCACCCTGCATGATATGCGGTACCAAAAGCTGATTGTGCAGATTTTA
  ATAGAAAACGAAGACGTTTTATTCTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004067 unedited
  NGGTGTCAAATTTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGGCGAG
  GGCAGCGGCGGCGGCGTCCGCACCGGGGCTGAGCGAGCAGCGACGCGAGGGGCGCGCGGA
  GATGGCAGCGTCCAGCAACTCCAGCCTGTCCGGCTCGTCGGTGTCCTCCGATGCTGAAGA
  ATACCAGCCTCCTATATGGAAATCATACTTATATCAGTTACAGCAAGAGGCACCTCGTCC
  CAAGAGAATCATTTGTCCTCGGGAGGTGGAAAACAGACCAAAATATTATGGAAGAGAGTT
  TCATGGGATCATCTCTCGGGAGCAGGCGGATGAGCTTCTTGGAGGCGTGGAGGGTGCCTA
  CATCCTTAGAGAAAGCCAGCGGCAACCAGGATGCTACACGCTGGCTCTCAGGTTTGGAAA
  CCAGACCTTAAACTACAGGCTCTTCCACGACGGGAAACACTTTGTGGGTGAGAAGAGGTT
  TGAGTCGATTCATGATCTGGTGACAGATGGCTTGATAACACTGTACATAGAAACAAAAGC
  TGCCGAGTACATTTCAAAAATGACAACTAACCCCATCTATGAACACATTGGATATGCCAC
  CCTACTCAGAGAAAAAGTATCCAGAAGGCTGAGCAGGTCTAAAAATGAACCAAGAAAAAC
  AAACGTCACACATGAAGAACACACAGCGGTGGAAAAGATCTCCTCCCTGGTTCGAAGGGC
  TGCCCTCACACACAACGACNACCACTTCATTATGAGAAGACACACAACTTTAAGTCCACA
  CGTTCCGAGGCCACACTGGTGTGAATATTGTGCCAATTTCATGTGGGGGCTCATCGCCCA
  GGGGTNNCGTGCTCAAACTGTGATTGACGTACACAACAGTGTCCAAGCACGTCCCATGAC
  TGCCACCTGATCTCAGAGATCAGNAAGTGTCTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004067
• Insert Size: 3160 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004067.1, NP_004058.1
• RefSeq Size: 2213 bp
• RefSeq ORF: 1407 bp
• Locus ID: 1124
• Cytogenetics: 7p14.3
• Domains: RhoGAP, SH2, DAG_PE-bind
• Gene Summary: 'This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that contains a phorbol-ester/diacylglycerol (DAG)-type zinc finger, a Rho-GAP domain, and an SH2 domain. The encoded protein translocates from the cytosol to the Golgi apparatus membrane upon binding by diacylglycerol (DAG). Activity of this protein is important in cell proliferation and migration, and expression changes in this gene have been detected in cancers. A mutation in this gene has also been associated with schizophrenia in men. Alternative transcript splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, May 2014]'
  Transcript Variant: This variant (2) differs in the 5' UTR and contains multiple additional 5' coding exons, compared to variant 1. It represents use of an alternate promoter and initiates translation from an alternate start codon. The encoded isoform (2) has a distinct N-terminus and is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.