AP2M1 (BC013796) Human Untagged Clone

CAT#: SC125671

AP2M1 (untagged)-Human adaptor-related protein complex 2, mu 1 subunit, mRNA (cDNA clone MGC:9329 IMAGE:3924339), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AP2M1
• Synonyms: AP50; CLAPM1; mu2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC013796 edited
  GGGCCGGCAGAGCAGGGGGCCGAGGACACCAGGTCTGTTCTCAGAGCGATGGGCCGCGGA
  GACTGATCTGCCGCCATGATTGGAGGCTTATTCATCTATAATCACAAGGGGGAGGTGCTC
  ATCTCCCGAGTCTACCGAGATGACATCGGGAGGAACGCAGTGGATGCCTTTCGGGTCAAT
  GTTATCCATGCCCGGCAGCAGGTGCGCAGCCCCGTCACCAACATTGCTCGCACCAGCTTC
  TTCCACGTTAAGCGGTCCAACATTTGGCTGGCAGCAGTCACCAAGCAGAATGTCAACGCT
  GCCATGGTCTTCGAATTCCTCTATAAGATGTGTGACGTGATGGCTGCCTACTTTGGCAAG
  ATCAGCGAGGAAAACATCAAGAACAATTTTGTGCTCATATATGAGCTGCTGGATGAGATT
  CTAGACTTTGGCTACCCACAGAATTCCGAGACAGGCGCGCTGAAAACCTTCATCACGCAG
  CAGGGCATCAAGAGTCAGACAAAAGAAGAGCAGTCACAGATCACCAGCCAGGTAACTGGG
  CAGATTGGCTGGCGGCGAGAGGGTATCAAGTATCGTCGGAATGAGCTCTTCCTGGATGTG
  CTGGAGAGTGTGAACCTGCTCATGTCCCCACAAGGGCAGGTGCTGAGTGCCCATGTGTCG
  GGCCGGGTGGTGATGAAGAGCTACCTGAGTGGCATGCCTGAATGCAAGTTTGGGATGAAT
  GACAAGATTGTTATTGAAAAGCAGGGCAAAGGCACAGCTGATGAAACAAGCAAGAGCGGG
  AAGCAATCAATTGCCATTGATGACTGCACCTTCCACCAGTGTGTGCGACTCAGCAAGTTT
  GACTCTGAACGCAGCATCAGCTTTATCCCGCCAGATGGAGAGTTTGAGCTTATGAGGTAT
  CGCACAACCAAGGACATCATCCTTCCCTTCCGGGTGATCCCGCTAGTGCGAGAAGTGGGA
  CGCACCAAACTGGAGGTCAAGGTGGTCATCAAGTCCAACTTTAAACCCTCACTGCTGGCT
  CAGAAGATTGAGGTGAGGATCCCAACCCCACTGAACACAAGCGGGGTGCAGGTGATCTGC
  ATGAAGGGGAAGGCCAAGTACAAGGCCAGCGAGAATGCCATCGTGTGGAAGATCAAGCGC
  ATGGCAGGCATGAAGGAATCGCAGATCAGCGCAGAGATTGAGCTTCTGCCTACCAACGAC
  AAGAAGAAATGGGCTCGACCCCCCATTTCCATGAACTTTGAGGTGCCATTCGCACCCTCT
  GGCCTCAAGGTGCGCTACTTGAAGGTGTTTGAACCGAAGCTGAACTACAGCGACCATGAT
  GTCATCAAATGGGTGCGCTACATTGGCCGCAGTGGCATTTATGAAACTCGCTGCTAGCTG
  CCACTAGGCAGCTAGCCCACCTCCCCAGCCACCCTCCTCCACAGGTCCAGGTGCCGCTCC
  CTCCCCCACCACACATCAGTGTCTCCTCCCTCCTGCTTTGCTGCCTTCCCTTTGCACCAG
  CCCGAGTCTAGGTCTGGGCCAAGCACATTACAAGTGGGACCGGTGGAGCAGCCCCTGGGC
  TCCCTGGGCAGGGGAGTTCTGAGGCTCCTGCTCTCCCATCCACCTGTCTGTCCTGGCCTA
  ATGCCAGGCTCTGAGTTCTGTGACCAAAGCCAGGTGGGTTCCCTTTCCTTCCCACCCCTG
  TGGCCACAGCTCTGGAGTGGGAGGGTTGGTTGCCCCTCACCTCAGAGCTCCCCCAAAGGC
  CAGTAATGGATCCCCGGCCTCAGTCCCTACTCTGCTTTGGGATAGTGTGAGCTTCATTTT
  GTACACGTGTGACTTCGTCCAGTTACAAACCCAATAAACTCTGTAGAGTGGAAAAAAAAA
  AAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC013796 unedited
  TTCTGGATTTGTAATACGACTCTATATAGGGCGGCCGCGNAATCAAATCTGGTACCGGTC
  CGGAATTCCCGGGATATCGTCGACCCACGCGTCCGCCCACGCGTCCGGGGCCGGCAGAGC
  AGGGGGCCGAGGACACCAGGTCTGTTCTCAGAGCGATGGGCCGCGGAGACTGATCTGCCG
  CCATGATTGGAGGCTTATTCATCTATAATCACAAGGGGGAGGTGCTCATCTCCCGAGTCT
  ACCGAGATGACATCGGGAGGAACGCAGTGGATGCCTTTCGGGTCAATGTTATCCATGCCC
  GGCAGCAGGTGCGCAGCCCCGTCACCAACATTGCTCGCACCAGCTTCTTCCACGTTAAGC
  GGTCCAACATTTGGCTGGCAGCAGTCACCAAGCAGAATGTCAACGCTGCCATGGTCTTCG
  AATTCCTCTATAAGATGTGTGACGTGATGGCTGCCTACTTTGGCAAGATCAGCGAGGAAA
  ACATCAAGAACAATTTTGTGCTCATATATGAGCTGCTGGATGAGATTCTAGACTTTGGCT
  ACCCACAGAATTCCGAGACAGGCGCGCTGAANACCTTCATCACGCAGCAGGGCATCAAGA
  GTCAGACAAAAGAAGAGCAGTCACAGATCACCAGCCAGGTAACTGGGCAGATTGGCTGGC
  GGCGAGAGGGTATCAAGTATCGTCNGAATGAGCTCTTCCTGGATGTGCTGGAGAGTGTGA
  ACCTGCTCATGTCCCCACAGGGGCAGGTGCTGAGTGCCCATGTGTCNGGCCGGGTGGTGA
  TGAAGAGCTACCTGAGTGGCATGCCTGAAATGCAGGTTGGGGATGAATNGACAGATTGTT
  ATTGAAAAGCAGGGCANAGGCACAGCTGATGAAANNCAGCAGAGCGGGGAGCAATCCATT
  GCCATTGATGACTGCACCCTTCACCANTGTGTGCGACTCANCAGTTTN
  
• Restriction Sites: Please inquire
• ACCN: BC013796
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC013796.1, AAH13796.1
• RefSeq Size: 1867 bp
• Locus ID: 1173
• Cytogenetics: 3q27.1
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Huntington's disease
• Gene Summary: 'This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015]'