AP2M1 (BC013796) Human Untagged Clone

CAT#: SC125671

AP2M1 (untagged)-Human adaptor-related protein complex 2, mu 1 subunit, mRNA (cDNA clone MGC:9329 IMAGE:3924339), complete cds


Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol AP2M1
Synonyms AP50; CLAPM1; mu2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for BC013796 edited
GGGCCGGCAGAGCAGGGGGCCGAGGACACCAGGTCTGTTCTCAGAGCGATGGGCCGCGGA
GACTGATCTGCCGCCATGATTGGAGGCTTATTCATCTATAATCACAAGGGGGAGGTGCTC
ATCTCCCGAGTCTACCGAGATGACATCGGGAGGAACGCAGTGGATGCCTTTCGGGTCAAT
GTTATCCATGCCCGGCAGCAGGTGCGCAGCCCCGTCACCAACATTGCTCGCACCAGCTTC
TTCCACGTTAAGCGGTCCAACATTTGGCTGGCAGCAGTCACCAAGCAGAATGTCAACGCT
GCCATGGTCTTCGAATTCCTCTATAAGATGTGTGACGTGATGGCTGCCTACTTTGGCAAG
ATCAGCGAGGAAAACATCAAGAACAATTTTGTGCTCATATATGAGCTGCTGGATGAGATT
CTAGACTTTGGCTACCCACAGAATTCCGAGACAGGCGCGCTGAAAACCTTCATCACGCAG
CAGGGCATCAAGAGTCAGACAAAAGAAGAGCAGTCACAGATCACCAGCCAGGTAACTGGG
CAGATTGGCTGGCGGCGAGAGGGTATCAAGTATCGTCGGAATGAGCTCTTCCTGGATGTG
CTGGAGAGTGTGAACCTGCTCATGTCCCCACAAGGGCAGGTGCTGAGTGCCCATGTGTCG
GGCCGGGTGGTGATGAAGAGCTACCTGAGTGGCATGCCTGAATGCAAGTTTGGGATGAAT
GACAAGATTGTTATTGAAAAGCAGGGCAAAGGCACAGCTGATGAAACAAGCAAGAGCGGG
AAGCAATCAATTGCCATTGATGACTGCACCTTCCACCAGTGTGTGCGACTCAGCAAGTTT
GACTCTGAACGCAGCATCAGCTTTATCCCGCCAGATGGAGAGTTTGAGCTTATGAGGTAT
CGCACAACCAAGGACATCATCCTTCCCTTCCGGGTGATCCCGCTAGTGCGAGAAGTGGGA
CGCACCAAACTGGAGGTCAAGGTGGTCATCAAGTCCAACTTTAAACCCTCACTGCTGGCT
CAGAAGATTGAGGTGAGGATCCCAACCCCACTGAACACAAGCGGGGTGCAGGTGATCTGC
ATGAAGGGGAAGGCCAAGTACAAGGCCAGCGAGAATGCCATCGTGTGGAAGATCAAGCGC
ATGGCAGGCATGAAGGAATCGCAGATCAGCGCAGAGATTGAGCTTCTGCCTACCAACGAC
AAGAAGAAATGGGCTCGACCCCCCATTTCCATGAACTTTGAGGTGCCATTCGCACCCTCT
GGCCTCAAGGTGCGCTACTTGAAGGTGTTTGAACCGAAGCTGAACTACAGCGACCATGAT
GTCATCAAATGGGTGCGCTACATTGGCCGCAGTGGCATTTATGAAACTCGCTGCTAGCTG
CCACTAGGCAGCTAGCCCACCTCCCCAGCCACCCTCCTCCACAGGTCCAGGTGCCGCTCC
CTCCCCCACCACACATCAGTGTCTCCTCCCTCCTGCTTTGCTGCCTTCCCTTTGCACCAG
CCCGAGTCTAGGTCTGGGCCAAGCACATTACAAGTGGGACCGGTGGAGCAGCCCCTGGGC
TCCCTGGGCAGGGGAGTTCTGAGGCTCCTGCTCTCCCATCCACCTGTCTGTCCTGGCCTA
ATGCCAGGCTCTGAGTTCTGTGACCAAAGCCAGGTGGGTTCCCTTTCCTTCCCACCCCTG
TGGCCACAGCTCTGGAGTGGGAGGGTTGGTTGCCCCTCACCTCAGAGCTCCCCCAAAGGC
CAGTAATGGATCCCCGGCCTCAGTCCCTACTCTGCTTTGGGATAGTGTGAGCTTCATTTT
GTACACGTGTGACTTCGTCCAGTTACAAACCCAATAAACTCTGTAGAGTGGAAAAAAAAA
AAAAAAA
>OriGene 5' read for BC013796 unedited
TTCTGGATTTGTAATACGACTCTATATAGGGCGGCCGCGNAATCAAATCTGGTACCGGTC
CGGAATTCCCGGGATATCGTCGACCCACGCGTCCGCCCACGCGTCCGGGGCCGGCAGAGC
AGGGGGCCGAGGACACCAGGTCTGTTCTCAGAGCGATGGGCCGCGGAGACTGATCTGCCG
CCATGATTGGAGGCTTATTCATCTATAATCACAAGGGGGAGGTGCTCATCTCCCGAGTCT
ACCGAGATGACATCGGGAGGAACGCAGTGGATGCCTTTCGGGTCAATGTTATCCATGCCC
GGCAGCAGGTGCGCAGCCCCGTCACCAACATTGCTCGCACCAGCTTCTTCCACGTTAAGC
GGTCCAACATTTGGCTGGCAGCAGTCACCAAGCAGAATGTCAACGCTGCCATGGTCTTCG
AATTCCTCTATAAGATGTGTGACGTGATGGCTGCCTACTTTGGCAAGATCAGCGAGGAAA
ACATCAAGAACAATTTTGTGCTCATATATGAGCTGCTGGATGAGATTCTAGACTTTGGCT
ACCCACAGAATTCCGAGACAGGCGCGCTGAANACCTTCATCACGCAGCAGGGCATCAAGA
GTCAGACAAAAGAAGAGCAGTCACAGATCACCAGCCAGGTAACTGGGCAGATTGGCTGGC
GGCGAGAGGGTATCAAGTATCGTCNGAATGAGCTCTTCCTGGATGTGCTGGAGAGTGTGA
ACCTGCTCATGTCCCCACAGGGGCAGGTGCTGAGTGCCCATGTGTCNGGCCGGGTGGTGA
TGAAGAGCTACCTGAGTGGCATGCCTGAAATGCAGGTTGGGGATGAATNGACAGATTGTT
ATTGAAAAGCAGGGCANAGGCACAGCTGATGAAANNCAGCAGAGCGGGGAGCAATCCATT
GCCATTGATGACTGCACCCTTCACCANTGTGTGCGACTCANCAGTTTN
Restriction Sites Please inquire     
ACCN BC013796
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC013796.1, AAH13796.1
RefSeq Size 1867 bp
Locus ID 1173
Cytogenetics 3q27.1
Protein Families Druggable Genome
Protein Pathways Endocytosis, Huntington's disease
Gene Summary 'This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015]'

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.