A RAF (ARAF) (BC009028) Human Untagged Clone

CAT#: SC125965

(untagged)-Human mRNA similar to raf-related oncogene (cDNA clone MGC:18177 IMAGE:4155318), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARAF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC009028 edited
  CCCACGCGTCCGAGGCGGTGGTGGTGGTAGCGGTGTGACAGGAGGCCTATGGCAGCTGCC
  CAGTCCTACCTCAGCCCATCTTGAAAACAATCTTAGGCAACATGGAACCACCACGAGGCC
  CTCCTGTTAGTGGGGCTGAGCCATCTCGGGCAGTTGGCACTGTCAAAGTGTACCTGCCTA
  ACAAGCAACGCACAGTGGTGACTGTCCGGGAAGGCATGAGTGTCTATGACTCTTTGGACA
  AGGCCCTGAAGGTGCGGGGTCTCAACCAGGACTGCTGTGTGGTCTACAGGCTCATCAAAG
  GAAGAAAGACAGTCACTGCCTGGGACACAGCCATTGCGCCTCTGGATGGTGAGGAGCTCA
  TTGTGGAGGTCCTAGAAGATGTGCCACTGACCATGCACAATTTTGTACGGAAGACATTCT
  TCAGCCTGGCCTTCTGTGACTTCTGCCTTAAGTTTCTGTTCCATGGATTCCGCTGCCAGA
  CCTGTGGCTACAAGTTCCACCAGCATTGTTCCTCCAAGGTCCCCACCGTCTGCGTTGACA
  TGAGTACCAACCGCCGACAGTTCTACCACAGCATCCAGGATCTGTCTGGAGGCTCCAGGC
  AGCAGGAGGCTCCCTCAAATCTCTCTGTGAATGAGCTGCTAACCCCCCAGGGTCCCAGGT
  AGGGATGTCGTAGATGAAAGGCCAGGGGGCCAGTGAAAGAAGGATTGGAGGACCCTGAGA
  GATTATACTTTCGTTTATTTGTTTACGTGATAAATACTATTGAGTACCTACAAGTGTTGA
  ATATGGGCATGGGATTGTGGGATCATTGGTAAGGCACCCAGTCACTCCAGGTTTCATTTT
  TTTCATCTGGGAAGAGGGGATGAGATTTTCCCTAGTGGGTTCTGAAGAGGATTAAGGGTT
  GAGATATTTGCAATGCTTAAAGCAAGATCTGATACAGAGTAAATAACAAGTGTTATTCAT
  TACAAGTGTATATATAAAATCATCTAGTTTGTTCATTTCTTCCAGTATAACCACACCCCA
  GTCTTTTCTCTTTGAAATAAATGTTACTGTATCAGAAAGTACTTTTATTTCACTTATGTT
  GGATATGATAGCATATAGTTTTGCATAATATATAAAAAGTAAGGTTAATGTATTATTATT
  TGTAGTTATGAGTGCATGTGTGTTGATTTACCTGTGAATTTAATCTCAGGATGGTGAAAG
  TAACAGAAGAATGTTTTTCTGTAAGGTATAATTCATGGGAGGTGGAGGCAAGACGATCAA
  TAGTTCAAGGCTACCCTCCACTAAGTAGCAAGTTCAAGGCCAGCCTGGGCTACATGAGAC
  TGTCTTAAAACAGTAAAAAAGGTGTTTTGTAACGTGATATATGTATAATCGTTATATGAT
  GTGTGTAATGTGTATTACATAATATAATATGTGTATTGAAAACGTTGCCATTTTAATATA
  GGTAATGCTCATTATTATAAAATAAATAATATATTTGAAAAATACATGTCATTATGATAT
  GTAATAAAAAGTCATATCTTACCCTTTAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC009028 unedited
  ACTTTGAATTTGTATACGACTTATATGGGCGGCCGCGNATTCAGATCTGGTACCGGTCCG
  GAATTCCCGGGATATCGTCGACCCACGCGTCCGCCCACGCGTCCGAGGCGGTGGTGGTGG
  TAGCGGTGTGACAGGAGGCCTATGGCAGCTGCCCAGTCCTACCTCAGCCCATCTTGAAAA
  CAATCTTAGGCAACATGGAACCACCACGAGGCCCTCCTGTTAGTGGGGCTGAGCCATCTC
  GGGCAGTTGGCACTGTCAAAGTGTACCTGCCTAACAAGCAACGCACAGTGGTGACTGTCC
  GGGAAGGCATGAGTGTCTATGACTCTTTGGACAAGGCCCTGAAGGTGCGGGGTCTCAACC
  AGGACTGCTGTGTGGTCTACAGGCTCATCAAAGGAAGAAAGACAGTCACTGCCTGGGACA
  CAGCCATTGCGCCTCTGGATGGTGAGGAGCTCATTGTGGAGGTCCTAGAAGATGTGCCAC
  TGACCATGCACAATTTTGTACGGAAGACATTCTTCAGCCTGGCCTTCTGTGACTTCTGCC
  TTAAGTTTCTGTTCCATGGATTCCGCTGCCAGACCTGTGGCTACAAGTTCCACCAGCATT
  GTTCCTCCAAGGTCCCCACCGTCTGCGTTGACATGAGTACCAACCGCCGACAGTTCTACC
  ACAGCATCCAGGATCTGTCTGGAGGCTCCAGGCAGCAGGAGGCTCCCTCAAATCTCTCTG
  TGAATGAGCTGCTAACCCCCCCAGGTCCCANNGTAGGATGTCGTANATGAAAGCCAGGGG
  GCCAGTGAAAGAAGGATTGGAGGACCCTGAGAGATATACCTTTCGTTATTGGTTACGTGA
  TAATACTATGAGTACCTACAGTGTTGATATGGGCATGGGATGGTGGATCATGGTAAAGCA
  CCCGTCATCCCAGTTTCATTTTTTTATA
  
• Restriction Sites: Please inquire
• ACCN: BC009028
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC009028.1, AAH09028.1
• RefSeq Size: 1542 bp
• Locus ID: 369
• Cytogenetics: Xp11.3
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Bladder cancer, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Glioma, Insulin signaling pathway, Long-term depression, Long-term potentiation, Melanoma, Natural killer cell mediated cytotoxicity, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Vascular smooth muscle contraction
• Gene Summary: 'This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012]'