AP2M1 (BC014030) Human Untagged Clone

CAT#: SC126263

AP2M1 (untagged)-Human adaptor-related protein complex 2, mu 1 subunit, mRNA (cDNA clone MGC:20211 IMAGE:4541249), complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AP2M1
• Synonyms: AP50; CLAPM1; mu2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC014030 edited
  GGGCAGACGAGCAGGGGGCGGGCGGACATCTTGGGATCCGGAGAGTGGCCGGGCCGGCAG
  AGCAGGGGGCCGAGGACACCAGGTCTGTTCTCAGAGCGATGGGCCGCGGAGACTGATCTG
  CCGCCATGATTGGAGGCTTATTCATCTATAATCACAAGGGGGAGGTGCTCATCTCCCGAG
  TCTACCGAGATGACATCGGGAGGAACGCAGTGGATGCCTTTCGGGTCAATGTTATCCATG
  CCCGGCAGCAGGTGCGCAGCCCCGTCACCAACATTGCTCGCACCAGCTTCTTCCACGTTA
  AGCGGTCCAACATTTGGCTGGCAGCAGTCACCAAGCAGAATGTCAACGCTGCCATGGTCT
  TCGAATTCCTCTATAAGATGTGTGACGTGATGGCTGCCTACTTTGGCAAGATCAGCGAGG
  AAAACATCAAGAACAATTTTGTGCTCATATATGAGCTGCTGGATGAGATTCTAGACTTTG
  GCTACCCACAGAATTCCGAGACAGGCGCGCTGAAAACCTTCATCACGCAGCAGGGCATCA
  AGAGTCAGCATCAGACAAAAGAAGAGCAGTCACAGATCACCAGCCAGGTAACTGGGCAGA
  TTGGCTGGCGGCGAGAGGGTATCAAGTATCGTCGGAATGAGCTCTTCCTGGATGTGCTGG
  AGAGTGTGAACCTGCTCATGTCCCCACAAGGGCAGGTGCTGAGTGCCCATGTGTCGGGCC
  GGGTGGTGATGAAGAGCTACCTGAGTGGCATGCCTGAATGCAAGTTTGGGATGAATGACA
  AGATTGTTATTGAAAAGCAGGGCAAAGGCACAGCTGATGAAACAAGCAAGAGCGGGAAGC
  AATCAATTGCCATTGATGACTGCACCTTCCACCAGTGTGTGCGACTCAGCAAGTTTGACT
  CTGAACGCAGCATCAGCTTTATCCCGCCAGATGGAGAGTTTGAGCTTATGAGGTATCGCA
  CAACCAAGGACATCATCCTTCCCTTCCGGGTGATCCCGCTAGTGCGAGAAGTGGGACGCA
  CCAAACTGGAGGTCAAGGTGGTCATCAAGTCCAACTTTAAACCCTCACTGCTGGCTCAGA
  AGATCGAGGTGAGGATCCCAACCCCACTGAACACAAGCGGGGTGCAGGTGATCTGCATGA
  AGGGGAAGGCCAAGTACAAGGCCAGCGAGAATGCCATCGTGTGGAAGATCAAGCGCATGG
  CAGGCATGAAGGAATCGCAGATCAGCGCAGAGATTGAGCTTCTGCCTACCAACGACAAGA
  AGAAATGGGCTCGACCCCCCATTTCCATGAACTTTGAGGTGCCATTCGCGCCCTCTGGCC
  TCAAGGTGCGCTACTTGAAGGTGTTTGAACCGAAGCTGAACTACAGCGACCATGATGTCA
  TCAAATGGGTGCGCTACATTGGCCGCAGTGGCATTTATGAAACTCGCTGCTAGCTGCCAC
  TAGGCAGCTAGCCCACCTCCCCAGCCACCCTCCTCCACAGGTCCAGGTGCCGCTCCCTCC
  CCCACCACACATCAGTGTCTCCTCCCTCCTGCTTTGCTGCCTTCCCTTTGCACCAGCCCG
  AGTCTAGGTCTGGGCCAAGCACATTACAAGTGGGACCGGTGGAGCAGCCCCTGGGCTCCC
  TGGGCAGGGGAGTTCTGAGGCTCCTGCTCTCCCATCCACCTGTCTGTCCTGGCCTAATGC
  CAGGCTCTGAGTTCTGTGACCAAAGCCAGGTGGGTTCCCTTTCCTTCCCACCCCTGTGGC
  CACAGCTCTGGAGTGGGAGGGTTGGTTGCCCCTCACCTCAGAGCTCCCCCAAAGGCCAGT
  AATGGATCCCCGGCCTCAGTCCCTACTCTGCTTTGGGATAGTGTGAGCTTCATTTTGTAC
  ACGTGTGACTTCGTCCAGTTACAAACCCAATAAACTCTGTAGAGTGGAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC014030 unedited
  GGGTTTAGATTTGTATACGACTTACTATAGGCGGCCGCGNATTCANATCTGGTACCGGTC
  CGGAATTCCCGGGATATCGTCGACCCACGCGTCCGCCCACGCGTCCGGGGCCGGCAGAGC
  AGGGGGCCGAGGACACCAGGTCTGTTCTCAGAGCGATGGGCCGCGGAGACTGATCTGCCG
  CCATGATTGGAGGCTTATTCATCTATAATCACAAGGGGGAGGTGCTCATCTCCCGAGTCT
  ACCGAGATGACATCGGGAGGAACGCAGTGGATGCCTTTCGGGTCAATGTTATCCATGCCC
  GGCAGCAGGTGCGCAGCCCCGTCACCAACATTGCTCGCACCAGCTTCTTCCACGTTAAGC
  GGTCCAACATTTGGCTGGCAGCAGTCACCAAGCAGAATGTCAACGCTGCCATGGTCTTCG
  AATTCCTCTATAAGATGTGTGACGTGATGGCTGCCTACTTTGGCAAGATCAGCGAGGAAA
  ACATCAAGAACAATTTTGTGCTCATATATGAGCTGCTGGATGAGATTCTAGACTTTGGCT
  ACCCACAGAATTCCGAGACAGGCGCGCTGAAAACCTTCATCACGCAGCAGGGCATCAAGA
  GTCAGACAAAAGAAGAGCAGTCACAGATCACCAGCCAGGTAACTGGGCAGATTGGCTGGC
  GGCGAGAGGGTATCAAGTATCGTCGGAATGAGCTCTTCCTGGATGTGCTGGAGAGTGTGA
  ACCTGCTCATGTCCCCACAAGGGCAAGTGCTGAGTGCCCATGTGTCGGGCCGGGTGGTGA
  TGAAGAGCTACCTGAGTGGCATGCCTGAATGCAAGTTGGGGATGAATGACAAGATTGTTA
  TTGAAAAACAGGGCAAGGCACCGCTGGAGAAACAAGCAAGACGGGAAGCAATCAATGCCA
  TTGGT
  
• Restriction Sites: Please inquire
• ACCN: BC014030
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC014030.1, AAH14030.1
• RefSeq Size: 1939 bp
• Locus ID: 1173
• Cytogenetics: 3q27.1
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Huntington's disease
• Gene Summary: 'This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015]'