PTS (NM_000317) Human Untagged Clone

CAT#: SC126853

PTS (untagged)-Human 6-pyruvoyltetrahydropterin synthase (PTS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTS
• Synonyms: PTPS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126853 sequence for NM_000317 edited (data generated by NextGen Sequencing)
  ATGAGCACGGAAGGTGGTGGCCGTCGCTGCCAGGCACAAGTGTCCCGCCGCATCTCCTTC
  AGCGCGAGCCACCGATTGTACAGTAAATTTCTAAGTGATGAAGAAAACTTGAAACTGTTT
  GGGAAATGCAACAATCCAAATGGCCATGGGCACAATTATAAAGTTGTGGTGACAGTACAT
  GGAGAGATTGACCCTGCTACGGGAATGGTTATGAATCTGGCTGATCTCAAAAAATATATG
  GAGGAGGCGATTATGCAGCCCCTTGATCATAAGAATCTGGATATGGATGTGCCATACTTT
  GCAGATGTGGTGAGCACGACTGAAAATGTAGCTGTTTATATCTGGGACAACCTCCAGAAA
  GTTCTTCCTGTAGGAGTTCTTTATAAAGTAAAAGTATACGAAACTGACAATAATATTGTG
  GTTTATAAAGGAGAATAG
  
  Clone variation with respect to NM_000317.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000317 unedited
  GTATTTGTATACGACNACTATAGGGCGGCCGCGAATTCGCACGAGGGGNGAAGATGAGCA
  CGGAAGGTGGTGGCCGTCGCTGCCAGGCACAAGTGTCCCGCCGCATCTCCTTCAGCGCGA
  GCCACCGATTGTACAGTAAATTTCTAAGTGATGAAGAAAACTTGAAACTGTTTGGGAAAT
  GCAACAATCCAAATGGCCATGGGCACAATTATAAAGTTGTGGTGACAGTACATGGAGAGA
  TTGACCCTGCTACGGGAATGGTTATGAATCTGGCTGATCTCAAAAAATATATGGAGGAGG
  CGATTATGCAGCCCCTTGATCATAAGAATCTGGATATGGATGTGCCATACTTTGCAGATG
  TGGTGAGCACGACTGAAAATGTAGCTGTTTATATCTGGGACAACCTCCAGAAAGTTCTTC
  CTGTAGGAGTTCTTTATAAAGTAAAAGTATACGAAACTGACAATAATATTGTGGTTTATA
  AAGGAGAATAGCTATTGGGGTTAGCATTGCACAAAGCCCAGTTTCTTTCTGTGTTTGAAA
  AAGATTTTGATCCCCTTGGAATATTAAGAGGTCAACACGTGATTGTTGTACGTACACATT
  GTGCTCTGGAGTGCCTATTTATTGAAATCATTGTAAGACCTGTTATAAATTTAAGTCTAT
  TTAAAACTAAACTTGTAATATACATCCTGAAAATCATTTAGAGAGTCTTTTATTTATAAA
  TTAAAAATCACTTCATTTTCACAAAANAAAAAAAAAAAAACTCGACTCTAGATTGCGGCC
  GCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGGCATCCTGTGNACCCTCCCAGTGC
  CTCTCTGGCCNTGNNAGTGCCCTNCAGTGCCACCAGCCTTGTCTATAAAATAAGTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000317
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000317.1, NP_000308.1
• RefSeq Size: 921 bp
• RefSeq ORF: 438 bp
• Locus ID: 5805
• Cytogenetics: 11q23.1
• Domains: PTPS
• Protein Families: Druggable Genome
• Protein Pathways: Folate biosynthesis, Metabolic pathways
• Gene Summary: 'The enzyme encoded by this gene catalyzes the elimination of inorganic triphosphate from dihydroneopterin triphosphate, which is the second and irreversible step in the biosynthesis of tetrahydrobiopterin from GTP. Tetrahydrobiopterin, also known as BH(4), is an essential cofactor and regulator of various enzyme activities, including enzymes involved in serotonin biosynthesis and NO synthase activity. Mutations in this gene result in hyperphenylalaninemia. [provided by RefSeq, Oct 2008]'