Calpain 3 (CAPN3) (NM_173090) Human Untagged Clone

CAT#: SC126949

CAPN3 (untagged)-Human calpain 3, (p94) (CAPN3), transcript variant 6

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CAPN3
• Synonyms: CANP3; CANPL3; LGMD2; LGMD2A; LGMDD4; LGMDR1; nCL-1; p94
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126949 sequence for NM_173090 edited (data generated by NextGen Sequencing)
  ATGGAGATCTGTGCAGATGAGCTCAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAG
  GACCTGAAGACACACGGGTTCACACTGGAGTCCTGCCGTAGCATGATTGCGCTCATGGAT
  ACAGATGGCTCTGGAAAGCTCAACCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAG
  GCCTGGCAGAAAATTTTCAAACACTATGACACAGACCAGTCCGGCACCATCAACAGCTAC
  GAGATGCGAAATGCAGTCAACGACGCAGGATTCCACCTCAACAACCAGCTCTATGACATC
  ATTACCATGCGGTACGCAGACAAACACATGAACATCGACTTTGACAGTTTCATCTGCTGC
  TTCGTTAGGCTGGAGGGCATGTTCAGAGCTTTTCATGCATTTGACAAGGATGGAGATGGT
  ATCATCAAGCTCAACGTTCTGGAGTGGCTGCAGCTCACCATGTATGCCTGA
  
  Clone variation with respect to NM_173090.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_173090 unedited
  NNGGGCTGCATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCACAGGC
  GATTGGTTTTAGTGGTAGGTGTGTGGGGATCTGTTCTGGTCATCTGGATGCTGGTCATCG
  GTGTGCAGTATTGATCAGGACCTGCAAACCCAAAAGCTTATGGGAGCTGGCACGTCACAA
  AAAGAAAAAAACCAAGCCACAGCCTGGCAGCTCTGATCAGGAAAGTGAGGAACAGCAACA
  ATTCCGGAACATTTTCAAGCAGATAGCAGGAGATGACATGGAGATCTGTGCAGATGAGCT
  CAAGAAGGTCCTTAACACAGTCGTGAACAAACACAAGGACCTGAAGACACACGGGTTCAC
  ACTGGAGTCCTGCCGTAGCATGATTGCGCTCATGGATACAGATGGCTCTGGAAAGCTCAA
  CCTGCAGGAGTTCCACCACCTCTGGAACAAGATTAAGGCCTGGCAGAAAATTTTCAAACA
  CTATGACACAGACCAGTCCGGCACCATCAACAGCTACGAGATGCGAAATGCAGTCAACGA
  CGCAGGATTCCACCTCAACAACCAGCTCTATGACATCATTACCATGCGGTACGCAGACAA
  ACACATGAACATCGACTTTGACAGTTTCATCTGCTGCTTCGTTAGGCTGGAGGGCATGTT
  CAGAGCTTTTCATGCATTTGACAAGGATGGAGATGGTATCATCAAGCTCAACGTTCTGGA
  GTGGCTGCAGCTCACCATGTATGCCTGAACCAAGCTGGCCTCATCCAAAGCCATGCAGGA
  TCACTCAGGATTTCAGTTTCACCCTCTATTTTCAAAGCCCTTTACCTCANAGGACCCAGC
  AGCTACACCCCTACAGGCTTCCAG
  
• Restriction Sites: Please inquire
• ACCN: NM_173090
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173090.1, NP_775113.1
• RefSeq Size: 1309 bp
• RefSeq ORF: 471 bp
• Locus ID: 825
• Cytogenetics: 15q15.1
• Protein Families: Druggable Genome, Protease
• Gene Summary: 'Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (6) differs in the 5' UTR and uses a downstream start codon, compared to variant 1. It encodes isoform e, which has a shorter N-terminus compared to isoform a.