GCDH (NM_013976) Human Untagged Clone

CAT#: SC126971

GCDH (untagged)-Human glutaryl-CoA dehydrogenase (GCDH), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GCDH
• Synonyms: ACAD5; GCD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_013976, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCCTGAGAGGCGTCTCCGTGCGGCTGCTGAGCCGCGGACCCGGCCTGCACGTCCTTCGCACGTGGG
  TCTCGTCGGCGGCGCAGACCGAGAAAGGCGGGAGAACACAGAGCCAACTGGCTAAGTCCTCGCGTCCCGA
  GTTTGACTGGCAGGACCCGCTGGTGCTGGAGGAGCAGCTGACCACAGATGAGATCCTCATCAGGGACACC
  TTCCGCACCTACTGCCAGGAGAGACTCATGCCTCGCATCCTGTTGGCCAATCGCAACGAAGTTTTTCATC
  GGGAGATCATTTCGGAGATGGGGGAGTTGGGTGTGCTGGGCCCCACCATCAAAGGATATGGCTGTGCTGG
  GGTTTCGTCTGTGGCCTATGGGCTCCTGGCCCGAGAGCTGGAGCGGGTGGACAGTGGCTACAGGTCGGCG
  ATGAGTGTCCAGTCCTCCCTCGTCATGCACCCTATCTATGCCTATGGCAGCGAGGAACAGCGGCAGAAGT
  ACCTGCCCCAGCTGGCCAAGGGGGAGCTCCTGGGCTGCTTCGGGCTCACAGAGCCCAACAGCGGAAGTGA
  CCCCAGCAGCATGGAGACCAGAGCCCACTACAACTCATCCAACAAGAGCTACACCCTCAATGGGACCAAG
  ACCTGGATCACGAACTCGCCTATGGCCGATCTGTTTGTAGTGTGGGCTCGGTGTGAAGATGGCTGCATTC
  GGGGCTTCCTGCTGGAGAAGGGGATGCGGGGTCTCTCGGCCCCCAGGATCCAGGGCAAGTTCTCGCTGCG
  GGCCTCAGCCACAGGCATGATCATCATGGACGGTGTGGAGGTGCCAGAGGAGAATGTGCTCCCTGGTGCA
  TCCAGCCTGGGGGGTCCCTTCGGCTGCCTGAACAACGCCCGGTACGGCATCGCGTGGGGCGTGCTTGGAG
  CTTCGGAGTTCTGCTTGCACACAGCCCGGCAGTACGCCCTCGACAGGATGCAGTTTGGTGTCCCACTGGC
  CAGGAACCAGCTGATTCAGAAGAAGCTGGCAGACATGCTCACTGAGATTACCCTGGGCCTTCACGCCTGC
  CTGCAGCTCGGCCGCTTGAAGGACCAGGACAAGGCTGCCCCCGAGATGGTTTCTCTGCTGAAGAGGAATA
  ACTGTGGGAAAGCCCTGGACATCGCCCGCCAGGCCCGAGACATGCTGGGGGGGAATGGGATTTCTGACGA
  GTATCACGTGATCCGGCACGCCATGAACCTGGAGGCCGTGAACACCTACGAAGTCGTTCAGATGTGTTCC
  TTAAAAAGAAGATGGAATTCTCTGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013976 unedited
  TGTATACGACTCACTATAGGCGGCCGCGAATTCGGCACGAGGGAACCGGGAGGTACTACC
  AGGTCGCCGTCGTTGCTCCGCTCGCTCTGAGAGAGCATGGCCCTGAGAGGCGTCTCCGTG
  CGGCTGCTGAGCCGCGGACCCGGCCTGCACGTCCTTCGCACGTGGGTCTCGTCGGCGGCG
  CAGACCGAGAAAGGCGGGAGAACACAGAGCCAACTGGCTAAGTCCTCGCGTCCCGAGTTT
  GACTGGCAGGACCCGCTGGTGCTGGAGGAGCAGCTGACCACAGATGAGATCCTCATCAGG
  GACACCTTCCGCACCTACTGCCAGGAGAGACTCATGCCTCGCATCCTGTTGGCCAATCGC
  AACGAAGTTTTTCATCGGGAGATCATTTCGGAGATGGGGGAGTTGGGTGTGCTGGGCCCC
  ACCATCAAAGGATATGGCTGTGCTGGGGTTTCGTCTGTGGCCTATGGGCTCCTGGCCCGA
  GAGCTGGAGCGGGTGGACAGTGGCTACAGGTCGGCGATGAGTGTCCAGTCCTCCCTCGTC
  ATGCACCCTATCTATGCCTATGGCAGCGAGGAACAGCGGCAGAAGTACCTGCCCCAGCTG
  GCCAACGGGGAGCTCCTGGGCTGCTTCGGGCTCACAGAGCCCAACAGCGGGAAGTGACCC
  CAGCAGCATGGAGACCAGAGCCCACTACAACTCATCCAACAAGAGCTACACCCTCAATGG
  GACCAAGACCTGGATCACGAACTCGCCTATGGCCGATCTGTTTGTAGTGTGGCCTCCGTG
  TGAAGATGCCTGCATTCGGNGCTTCCTGCTGGAGAAGGGATGCGGGTCTCTCGCCCCCCA
  GATCAGNGGCAGTCTCGCTGCGGCCTCAGCACAGGCTGACATATGGACGTGTGGAGTGCC
  CAGGAGATTGCTCCCTGTGCATCAGCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013976 unedited
  TAGCTTGNACCCGCCCGCTTTCTAGNGATCAGTTTTCCTTTTTTTTTTTTTCAAAGTGCA
  GGTCTTTATTTTCCTCTCCCTCACTCTGCTCCAACACCCAGCATAAGGCACTACCCCCAG
  ATGGGAGGGAGGGAGGGCGCACTGTGAACTCAGTGTGAGGGGGTCAGCTGCAGCAGACGG
  AGTTGCTTCCATGTCACTCTCCTCTCAAGAGAAGCTGCTATTTCAGGGTAAATGGAGTCT
  GCTCTCATCCATGGTTAAAAGTGGATTGAGACGCTCTACAGAGAATTCCATCTTCTTTTT
  AAGGAACACATCCGAACGACTTCAGAAGGGAAATTTTGATATTTAAAAATCAGAGTCTCT
  CACTTCCCACTCCATCCGCCACCTCCCTTTCTAAGCTCAGAGCACAGCGCTCCTACGGTC
  CAGCCAGGCATCTCTCCAGAAAGGGGCTTGAGAGTTTCGGGCCCCTGATGGAGCGGCTCA
  CTTGCTGGCCGTGAACGCCTGGATTCCCGTGATAGCTCTCCCAAGGATCAGGGCGTGAAT
  GCCATGTGTACCTTCGTAGGTGTTCACGGCCTCCAGGTTCATGGCGTGCCGGATCACGTG
  ATACTCGTCAGAAATCCCATTCCCCCCCAGCATGTCTCGGGCCTGGCGGGCGATGTCCAG
  GGCTTTCCCACAGTTATTCCTCTTCAGCAGAGAAACCATCTCGGGGGCAGCCTTGTCCTG
  GTCCTTCAAGCGGCCGAGCTGCAGGCAGGCGTGAAGGCCCAGGTAATCTCAGTGAGCATG
  TCTGCCAGCTTCTTCTGATCAGCTGGCTCTGGCCAGTGGAACACAACTGCATCTGTCGAG
  GCGTCTGCCCGGCTGTGTGCAGCATACTCCGAGCTCAGCCACGCCCACCGATGCGACCGG
  GCGTTGTCAGGACCCGAAGGCCCCCAGCCTGTAGCCCAGGAACCATCCTCTCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013976
• ORF Size: 1287 bp
• Insert Size: 1830
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_013976.2, NP_039663.1
• RefSeq Size: 1607
• RefSeq ORF: 1287
• Locus ID: 2639
• Domains: Acyl-CoA_dh, Acyl-CoA_dh_M, Acyl-CoA_dh_N
• Protein Families: Druggable Genome
• Protein Pathways: Fatty acid metabolism, Lysine degradation, Metabolic pathways, Tryptophan metabolism
• Gene Summary: The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Mutations in this gene result in the metabolic disorder glutaric aciduria type 1, which is also known as glutaric acidemia type I. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 12. [provided by RefSeq, Mar 2013]
  Transcript Variant: This variant (2) lacks a segment that results in a frameshift in the 3' coding region, compared to variant 1. The resulting isoform (b, also known as the short isoform) has a distinct and shorter C-terminus, compared to isoform a. This variant lacks publicly available transcript support but is supported by data in PubMed ID:8541831.