BBS4 (NM_033028) Human Untagged Clone

CAT#: SC127001

BBS4 (untagged)-Human Bardet-Biedl syndrome 4 (BBS4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BBS4
• Synonyms: Bardet-Biedl syndrome 4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_033028 edited
  AAATGGCTGAGGAGAGAGTCGCGACGAGAACTCAATTTCCTGTATCTACTGAGTCTCAAA
  AACCCCGGCAGAAAAAAGCTCCAGAGTTTCCTATTTTGGAGAAGCAGAACTGGTTGATTC
  ATCTTCATTATATCCGGAAAGATTATGAAGCCTGCAAGGCTGTTATCAAAGAACAGCTTC
  AAGAGACTCAGGGATTGTGTGAATATGCTATCTATGTCCAAGCATTGATATTTCGCCTAG
  AAGGAAATATCCAAGAATCCCTAGAACTCTTCCAGACATGTGCAGTTCTTAGTCCTCAGA
  GTGCTGATAACCTCAAGCAGGTGGCCAGATCTTTATTTCTTTTGGGAAAACATAAAGCTG
  CCATTGAAGTATATAATGAAGCAGCTAAACTCAACCAGAAAGATTGGGAGATCAGCCATA
  ACCTAGGAGTTTGCTACATATACCTGAAGCAGTTCAACAAGGCACAAGACCAGTTGCACA
  ATGCCCTGAATCTTAATAGGCACGATCTGACTTATATAATGCTGGGGAAGATCCACTTGC
  TGGAGGGAGACTTGGACAAGGCCATTGAAGTCTACAAGAAAGCAGTGGAGTTCTCACCAG
  AAAATACAGAGCTTCTTACAACTTTAGGATTACTCTACTTACAGCTCGGCATTTACCAGA
  AGGCATTTGAACATCTTGGCAATGCACTGACTTATGACCCTACCAACTACAAGGCCATCT
  TGGCAGCAGGCAGCATGATGCAGACCCACGGGGACTTTGATGTTGCCCTCACCAAATACA
  GAGTTGTGGCTTGTGCTGTTCCAGAAAGTCCTCCACTCTGGAATAACATTGGAATGTGTT
  TCTTTGGCAAGAAGAAATATGTGGCGGCCATCAGCTGCCTGAAACGAGCCAACTACTTGG
  CACCCTTCGATTGGAAGATTCTGTATAATTTGGGCCTTGTCCATTTGACCATGCAGCAGT
  ATGCATCAGCTTTTCATTTTCTCAGTGCGGCCATCAACTTCCAGCCAAAGATGGGGGAGC
  TCTACATGCTCTTGGCAGTGGCTCTGACCAATCTGGAAGATACAGAAAATGCCAAGAGAG
  CCTACGCAGAAGCAGTCCACCTGGATAAGTGTAACCCTTTAGTAAACCTGAACTATGCTG
  TGCTGCTGTACAACCAGGGCGAGAAGAAGAACGCCCTGGCCCAATATCAGGAGATGGAGA
  AGAAAGTCAGCCTACTCAAGGACAATAGCTCTCTGGAATTTGACTCTGAGATGGTGGAGA
  TGGCTCAGAAGTTGGGAGCTGCTCTCCAGGTTGGGGAGGCACTGGTCTGGACCAAACCAG
  TTAAAGATCCCAAATCAAAGCACCAGACCACTTCAACCAGCAAACCTGCCAGTTTCCAGC
  AGCCTCTGGGCTCTAATCAAGCTCTAGGACAGGCAATGTCTTCAGCAGCTGCATACAGGA
  CGCTCCCCTCAGGTGCTGGAGGAACATCCCAGTTCACAAAGCCCCCATCTCTTCCTCTGG
  AGCCAGAGCCTGCGGTGGAATCAAGTCCAACTGAAACATCAGAACAAATAAGAGAGAAAT
  AAGAATAGAATGAATGACCCCAAAATAGGGTTTTCTTGGGCGAGGATGTGCTGGATTAGG
  AAAGGTGACATGACACAGGCAGAGCAGAGTGGCACCCACCACAGAATACAGTGTGTGTTA
  TTACGAGGAGCCAGCAGTTGAGCCTAAGGTCCTTCTACCTACCTGGTATTGGCATTTGAG
  GTCGGAAACCCTCTACTGCCCCATAAGCCAGGAAAAGTGAAAAGAGAACACAGTTCCTTT
  AAGAACTGGCAGCAAGGCTTGAGGCCTTATGTATGTAGCTGAGTCAGCAAGGTACATGAT
  GCTGTCTGCTTTCAAAAGGACTTTTCTCTCCTAGCTGACTGACTCCTTCCTTAGTTCAAG
  GAACAGCTGAGACAGACCTCTGCTGAGTAGCTCTGTGATGACAAAGCCTTGGTTTAACTG
  AGGTGATCCTCAGGTTGTGAGGTTTATTAGTCCCCAAGGCAAACACAAATATTAGATTAA
  TAATCCAACTTTAATAGTATACATTTAAAAGAAAAAAAACAAAAGCCCTGGAAGTTGAGG
  CCAAGCCTGCTGAGTATTGCAGCTGCATTTGCCCAAAGGGAATCCAGAACAAGTCCCTCC
  CTGTATTTTGTTCTTGAGAGGGGTCAGTCTAGAAGCTAGATCCTATCAGGATGAGGAGCA
  GCAGCCCAGGGCTTGTCTGGATCAGCACCAACGATTTTAAAGAAAAAAGGAAGAGTTTCT
  TAGATGAGTAATTGTTATTGAAGATAGTCAGTGATAACCACTGACCAGATGCTATCAATA
  CACTATGTGTCCTTTTTAGAATAAAGATTACATATCATCATTCCTTTGGGGAAAATTGTT
  ATTCAGG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033028 unedited
  TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAATGGCTGAGGAGAGAGTCGC
  GACGAGAACTCAATTTCCTGTATCTACTGAGTCTCAAAAACCCCGGCAGAAAAAAGCTCC
  AGAGTTTCCTATTTTGGAGAAGCAGAACTGGTTGATTCATCTTCATTATATCCGGAAAGA
  TTATGAAGCCTGCAAGGCTGTTATCAAAGAACAGCTTCAAGAGACTCAGGGATTGTGTGA
  ATATGCTATCTATGTCCAAGCATTGATATTTCGCCTAGAAGGAAATATCCAAGAATCCCT
  AGAACTCTTCCAGACATGTGCAGTTCTTAGTCCTCAGAGTGCTGATAACCTCAAGCAGGT
  GGCCAGATCTTTATTTCTTTTGGGAAAACATAAAGCTGCCATTGAAGTATATAATGAAGC
  AGCTAAACTCAACCAGAAAGATTGGGAGATCAGCCATAACCTAGGAGTTTGCTACATATA
  CCTGAAGCAGTTCAACAAGGCACAAGACCAGTTGCACAATGCCCTGAATCTTAATAGGCA
  CGATCTGACTTATATAATGCTGGGGAAGATCCACTTGCTGGAGGGAGACTTGGACAAGGC
  CATTGAAGTCTACAAGAAAGCAGTGGAGTTCTCACCAGAAAATACAGAGCTTCTTACAAC
  TNTANGATTACTCTACTTACAGCTCGGCATTTACCAGAAGGCATTTGAACATCTTGGGCA
  TGCACTGACTTATGACCCTACCCAACTACAGGCCATCTTGGCAGCAGGCAGCATGATGCA
  AACCACGGNGACTNTGATGTTGCCCTCACCAATACANGAGTGGGCTTGTGCCTGTCANAA
  AGTCCTCCCTCTGGNNATACATTGGATGGTGTTCTTTGGCAGAAGAATATGTGGCGGCAT
  CAGCTGCTGAACGAGCCACTACTGGCACCCTCGATGGAAGATCTGATATTTGGGCCTGTC
  CATTGACCTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033028 unedited
  GCGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTAGGTTCTTTTAGGTTTTTAT
  TATGATCTCTTGTTTTTATACCCTGATAACAATTTTCCCCCAAAGGAATGATGATATGTA
  ATCTTTATTCTAAAAAGGACACATAGTGTATTGATAGCATCTGGTCAGTGGTTATCACTG
  ACTATCTTCAATAACAATTACTCATCTAAGAAACTCTTCCTTTTTTCTTTAAAATCGTTG
  GTGCTGATCCAGACAAGCCCTGGGCTGCTGCTCCTCATCCTGATAGGATCTAGCTTCTAG
  ACTGACCCCTCTCAAGAACAAAATACAGGGAGGGACTTGTTCTGGATTCCCTTTGGGCAA
  ATGCAGCTGCAATACTCAGCAGGCTTGGCCTCAACTTCCAGGGCTTTTGTTTTTTTTCTT
  TTAAATGTATACTATTAAAGTTGGATTATTAATCTAATATTTGNGTTTGCCTTGGGGACT
  AATAAACCTCACAACCTGAGGATCACCTCAGTTAAACCAAGGCTTTGTCATCACAGAGCT
  ACTCAGCAGAGGTCTGTCTCAGCTGTTCCTTGAACTAAGGAAGGAGTCAGTCAGCTAGGA
  GAGAAAAGTCCTTTTGAAAGCAGACAGCATCATGTACCTTGCTGACTCAGCTACCATACA
  TAAAGCCTTAAGCCCTTGCTTGCCAGTTCTTAAAGGAACCGTGGTCTCTTTTCACTTTTC
  CCTGGCTAATGGGCACGAAAAGGGTTCCGACCTCAAATGCCAAACCCAGGAGGTTAGAAG
  AACTTTAGCTAAATGCTGGCCCCCCCAAAACCCACCTGGATCCTGGGGGGNGCCACCTTG
  TTTTGCCTGACATGGCACCTTCCTAACCCCCCAATCCTGCCCAAAAAACCCTATTGGGGG
  GACCAATTCAACCCTATTCCCTTTATCGGTCCAGGTTAACGGGCCCGATCCCCCCCGCCT
  TCGCCTCAAAAAAAAATGGGCTCTTAAACCGAATCCCCCCCACCGAGGGAACCCTTGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033028
• Insert Size: 2400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033028.2, NP_149017.2
• RefSeq Size: 2482 bp
• RefSeq ORF: 1560 bp
• Locus ID: 585
• Cytogenetics: 15q24.1
• Domains: TPR
• Gene Summary: 'This gene is a member of the Bardet-Biedl syndrome (BBS) gene family. Bardet-Biedl syndrome is an autosomal recessive disorder characterized by severe pigmentary retinopathy, obesity, polydactyly, renal malformation and cognitive disability. The proteins encoded by BBS gene family members are structurally diverse. The similar phenotypes exhibited by mutations in BBS gene family members are likely due to the protein's shared roles in cilia formation and function. Many BBS proteins localize to the basal bodies, ciliary axonemes, and pericentriolar regions of cells. BBS proteins may also be involved in intracellular trafficking via microtubule-related transport. The protein encoded by this gene has sequence similarity to O-linked N-acetylglucosamine (O-GlcNAc) transferases in plants and archaebacteria and in human forms a multi-protein "BBSome" complex with seven other BBS proteins. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).