Artemis (DCLRE1C) (NM_022487) Human Untagged Clone

CAT#: SC127058

DCLRE1C (untagged)-Human DNA cross-link repair 1C (DCLRE1C), transcript variant b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DCLRE1C
• Synonyms: A-SCID; DCLREC1C; RS-SCID; SCIDA; SNM1C
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127058 sequence for NM_022487 edited (data generated by NextGen Sequencing)
  ATGAAGCATCAGGAGAGGTTTTTATTTCAGGGCAATAATGGAACTGTCCTGTACACAGGA
  GACTTCAGATTGGCGCAAGGAGAAGCTGCTAGAATGGAGCTTCTGCACTCCGGGGGCAGA
  GTCAAAGACATCCAAAGTGTATATTTGGATACTACGTTCTGTGATCCAAGATTTTACCAA
  ATTCCAAGTCGGGAGGAGTGTTTAAGTGGAGTCTTAGAGCTGGTCCGAAGCTGGATCACT
  CGGAGCCCGTACCATGTTGTTTGGCTGAACTGCAAAGCGGCTTATGGCTATGAATATCTG
  TTCACCAACCTTAGTGAAGAATTAGGAGTCCAGGTTCATGTGAATAAGCTAGACATGTTT
  AGGAACATGCCTGAGATCCTTCATCATCTCACAACAGACCGCAACACTCAGATCCATGCA
  TGCCGGCATCCCAAGGCAGAGGAATATTTTCAGTGGAGCAAATTACCCTGTGGAATTACT
  TCCAGAAATAGAATTCCACTCCACATAATCAGCATTAAGCCATCCACCATGTGGTTTGGA
  GAAAGGAGCAGAAAAACAAATGTAATTGTGAGGACTGGAGAGAGTTCATACAGAGCTTGT
  TTTTCTTTTCACTCCTCCTACAGTGAGATTAAAGATTTCTTGAGCTACCTCTGTCCTGTG
  AACGCATATCCAAATGTCATTCCAGTTGGCACAACTATGGATAAAGTTGTCGAAATCTTA
  AAGCCTTTATGCCGGTCTTCCCAAAGTACGGAGCCAAAGTATAAACCACTGGGAAAACTG
  AAGAGAGCTAGAACAGTTCACCGAGACTCAGAGGAGGAAGATGACTATCTCTTTGATGAT
  CCTCTGCCAATACCTTTAAGGCACAAAGTTCCATACCCGGAAACTTTTCACCCTGAGGTA
  TTTTCAATGACTGCAGTATCAGAAAAGCAGCCTGAAAAACTGAGACAAACCCCAGGATGC
  TGCAGAGCAGAGTGTATGCAGAGCTCTCGTTTCACAAACTTTGTAGATTGTGAAGAATCC
  AACAGTGAAAGTGAAGAAGAAGTAGGAATCCCAGCTTCACTGCAAGGAGATCTGGGCTCT
  GTACTTCACCTGCAAAAGGCTGATGGGGATGTACCCCAGTGGGAAGTATTCTTTAAAAGA
  AATGATGAAATCACAGATGAGAGTTTGGAAAACTTCCCTTCCTCCACAGTGGCAGGGGGA
  TCTCAGTCACCAAAGCTTTTCAGTGACTCTGATGGAGAATCAACTCACATCTCCTCCCAG
  AATTCTTCCCAGTCAACACACATAACAGAACAAGGAAGTCAAGGCTGGGACAGCCAATCT
  GATACTGTTTTGTTATCTTCCCAAGAGAGAAACAGTGGGGATATTACTTCCTTGGACAAA
  GCTGACTACAGACCAACAATCAAAGAGAATATTCCTGCCTCTCTCATGGAACAAAATGTA
  ATTTGCCCAAAGGATACTTACTCTGATTTGAAAAGCAGAGATAAAGATGTGACAATAGTT
  CCTAGTACTGGAGAACCAACTACTCTAAGCAGTGAGACACATATACCCGAGGAAAAAAGT
  TTGCTAAATCTTAGCACAAATGCAGATTCCCAGAGCTCTTCTGATTTTGAAGTTCCCTCA
  ACTCCAGAAGCTGAGTTACCTAAACGAGAGCATTTACAATATTTATATGAGAAGCTGGCA
  ACTGGTGAGAGTATAGCAGTCAAAAAAAGAAAATGCTCACTCTTAGATACCTAA
  
  Clone variation with respect to NM_022487.2
  261 g=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022487 unedited
  TTGTAATCCGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGTTTTGGGGTCC
  CGGACTCTGGGATCGGCGGCGCTATGAGTTCTTTCGAGGGGCAGATGGCCGAGTATCCAA
  CTATCTCCATAGACCGCTTCGATAGGGAGAACCTGAGGGCCCGCGCCTACTTCCTGTCCC
  ACTGCCACAAAGATCACATGAAAGGATTAAGAGCCCCTACCTTGAAAAGAAGGTTGGAGT
  GCAGATGAGGAAACTGAGGAACCAGAGAGGTTAAATTAGTTGCCCAGTGTCACACAGCCA
  GTAAGTGGCAGGACCAGGATTCCAGCCAGCTTGAAGGTTTATCTATACTGTTCACCTGTG
  ACTAAGGAGTTGTTGTTAACGAGCCCGAAATACAGATCTTGGAAGAAACGAATTATATCT
  ATTGAAATCGAGACTCCTACCCAGATATCTTTAGTGGATGAAGCATCAGGAGAGAAGGAA
  GAGATTGTTGTGACTCTCTTACCAGCTGGTCACTGTCCGGGATCAGTTAAGTTTTTATTT
  CAGGGCAATAATGGAACTGTCCTGTACACAGGAGACTTCAGATTGGCGCAAGGAGAAACT
  GCTAGAATGGAGCTTCTGCACTCCGGGGGCAGAGTCAAAGACATCCAAAGTGTATACTTG
  GATACTACGTTCTGTGATCCCCGAATTACCAAATTCCAAGTCCGGAGGCGTGGTTAAGTG
  GGAGTCTTACAGCTGGTCCGAAGCTGGATCACTCGGAGCCCGTACCATGTTGNACGGGCT
  GAACTGCAAACCGCCTTATTGCTTATGAATTCTTGTTCCCCACCCTTATGAACTATTAGG
  ATCCAGGGTCTTGTGAATAAACTACAATGTTCAGGAACTTGCTGAATATCCTTATTATTT
  TAAACAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022487 unedited
  TTACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTAAGTAGAGACACA
  TTTCACTGTGTTGGCCAGGCTGGTGTCGAACTCCTGGGCTCAAGCCATTGCCCACCTCAA
  AGTGCTGGGATTACAAGTGTGAGCCACCACACCCAACCAGGTTATTTGAACATTTTTAAG
  TACTGTATTTTCTCTATTGTAATATTGACTGTCATCTCTGTGCAGGTTTTTTAGTGGTTG
  CTCTAGGTTGAAACGCTTTGAATTCTTAGGTATCTAAGAGTGAGCATTTTCTTTTTTTGA
  CTGCTATACTCTCACCAGTTGCCAGCTTCTCATATAAATATTGTAAATGCTCTCGTTTAG
  GTAACTCAGCTTCTGGAGTTGAGGGAACTTCAAAATCAGAAGAGCTCTGGGAATCTGCAT
  TTGTGCTAAGATTTAGCAAACTTTTTTCCTCGGGTATATGTGTCTCACTGCTTAGAGTAG
  TTGGTTCTCCAGTACTAGGAACTATTGTCACATCTTTATCTCTGCTTTTCAAATCAGAGT
  AAGTATCCTTTGGGCAAATTACATTTTGTTCCATGAGAGAGGCAGGAATATTCTCTTTGA
  TTGTTGGTCTGTAGTCAGCTTTGTCCAAGGAAGTAATATCCCCACTGTTTCTCTCTTGGG
  AAGATAACANAACAGTATCAGATTGGCTGTCCCAGCCTTGACTTCCTTGTTCTGTTATGT
  GTGTTGACTGGGAAGAATTCTGGGAGGAGATGTGAGTTGATTCTCCATCAGAGTCACCTG
  AAAGCCTTGGTGACTGAGATCCCCCTGCCACTGTGGAGGAAGGNAAAGTTTTCAACTCTC
  ATCTGGGATTTATCATTCCTTTTAAGAAACTNCCACTGGGGTACATCCCATCAGCCTTTG
  CAGTGGAGTACAAAGCCAGATCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022487
• ORF Size: 1734 bp
• Insert Size: 2440
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_022487.1, NP_071932.1
• RefSeq Size: 2636
• RefSeq ORF: 1734
• Locus ID: 64421
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Non-homologous end-joining, Primary immunodeficiency
• Gene Summary: This gene encodes a nuclear protein that is involved in V(D)J recombination and DNA repair. The encoded protein has single-strand-specific 5'-3' exonuclease activity; it also exhibits endonuclease activity on 5' and 3' overhangs and hairpins. The protein also functions in the regulation of the cell cycle in response to DNA damage. Mutations in this gene can cause Athabascan-type severe combined immunodeficiency (SCIDA) and Omenn syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
  Transcript Variant: This variant (b, also called V3) lacks an exon in the 5' coding region and initiates translation at an alternate downstream start codon, compared to variant a. It encodes isoform b, which has a shorter and distinct N-terminus compared to variant a. Variants b, e, and g encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.