SMAD6 (NM_005585) Human Untagged Clone

CAT#: SC127117

SMAD6 (untagged)-Human SMAD family member 6 (SMAD6), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SMAD6
• Synonyms: AOVD2; HsT17432; MADH6; MADH7
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005585, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTCAGGTCCAAACGCTCGGGGCTGGTGCGGCGACTTTGGCGAAGTCGTGTGGTCCCCGACCGGGAGG
  AAGGCGGCAGCGGCGGCGGCGGTGGCGGCGACGAGGATGGGAGCTTGGGCAGCCGAGCTGAGCCGGCCCC
  GCGGGCAAGAGAGGGCGGAGGCTGCGGCCGCTCCGAAGTCCGCCCGGTAGCCCCGCGGCGGCCCCGGGAC
  GCAGTGGGACAGCGAGGCGCCCAGGGCGCGGGGAGGCGCCGGCGCGCAGGGGGCCCCCCGAGGCCCATGT
  CGGAGCCAGGGGCCGGCGCTGGGAGCTCCCTGCTGGACGTGGCGGAGCCGGGAGGCCCGGGCTGGCTGCC
  CGAGAGTGACTGCGAGACGGTGACCTGCTGTCTCTTTTCGGAGCGGGACGCCGCCGGCGCGCCCCGGGAC
  GCCAGCGACCCCCTGGCCGGGGCGGCCCTGGAGCCGGCGGGCGGCGGGCGGAGTCGCGAAGCGCGCTCGC
  GGCTGCTGCTGCTGGAGCAGGAACTCAAAACCGTCACGTACTCGCTGCTGAAGCGGCTCAAGGAGCGCTC
  GCTGGACACGCTGCTGGAGGCGGTGGAGTCCCGCGGCGGCGTGCCGGGCGGCTGCGTGCTGGTGCCGCGC
  GCCGACCTCCGCCTGGGCGGCCAGCCCGCGCCGCCGCAGCTGCTGCTCGGCCGCCTCTTTCGCTGGCCCG
  ACCTGCAGCACGCCGTGGAGCTGAAGCCCCTGTGCGGCTGCCACAGCTTCGCCGCCGCCGCCGACGGCCC
  TACCGTGTGCTGCAACCCCTACCACTTCAGCCGGCTCTGCGGGCCCGAATCTCCGCCACCTCCCTACTCT
  CGGCTGTCTCCTCGCGACGAGTACAAGCCACTGGATCTGTCCGATTCCACATTGTCTTACACTGAAACGG
  AGGCTACCAACTCCCTCATCACTGCTCCGGGTGAATTCTCAGACGCCAGCATGTCTCCGGACGCCACCAA
  GCCGAGCCACTGGTGCAGCGTGGCGTACTGGGAGCACCGGACGCGCGTGGGCCGCCTCTATGCGGTGTAC
  GACCAGGCCGTCAGCATCTTCTACGACCTACCTCAGGGCAGCGGCTTCTGCCTGGGCCAGCTCAACCTGG
  AGCAGCGCAGCGAGTCGGTGCGGCGAACGCGCAGCAAGATCGGCTTCGGCATCCTGCTCAGCAAGGAGCC
  CGACGGCGTGTGGGCCTACAACCGCGGCGAGCACCCCATCTTCGTCAACTCCCCGACGCTGGACGCGCCC
  GGCGGCCGCGCCCTGGTCGTGCGCAAGGTGCCCCCCGGCTACTCCATCAAGGTGTTCGACTTCGAGCGCT
  CGGGCCTGCAGCACGCGCCCGAGCCCGACGCCGCCGACGGCCCCTACGACCCCAACAGCGTCCGCATCAG
  CTTCGCCAAGGGCTGGGGGCCCTGCTACTCCCGGCAGTTCATCACCTCCTGCCCCTGCTGGCTGGAGATC
  CTCCTCAACAACCCCAGATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005585 unedited
  NACCCAGTATATCACCCTCCCCTTGACGCAAAGGGCGGTAGGCGTGTACGGTGGGCAGGT
  CTATATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGC
  AGCGGCCGCGAATTCGGCACGAGGGTTTTGTTTGGGATTTCCTTTTCTTTCTTTCCTTTT
  TTTTTTCTTTTTGCAGGGAGTAAGAAGGGAGCTGGGGGTATCAACAAGCCTGCCTTTCGG
  ATCCTGCGGGAAAAGCCCATGTAGTTAAGCGCTTTGGTTTAAAAAAAAGGCAAGGTAAAG
  GCAGGGCTTTCCAGACACATTTAGGGGTTCGCGCGAGCGCTTTGTGCTCATGGACCAGCC
  GCACAACTTTTGAAGGCTCGCCGGCCCATGTGGGGTCTTTCTGGCGGCGCGCCGCCTGCA
  GCCCCCCTAAAGCGCGGGGGCTGGAGTTGTTGAGCAGCCCCGCCGCTGTGGTCCATGTAG
  CCGCTGGCCGCGCGCGGACTGCGGCTCGGCGTGCGCGTGTTCCCGGCCGTCCCGCCTCGG
  CGAGCTCCCTCATGTTGTCGCCCTGCGGCGCCCCTTCGACGACAGGCTGTGCGCGGTCTG
  CACGGCGCTCCGCGGCGGAGCTTCATGTGGGGCTGCGACCCGCGCAGCCGGCGCCTCGCT
  GAGGGAACCGACCCCCGGTAACCGGAGACCGCCTCCCCCCCACCCCTGGCGCCAAAGGAT
  ATCGTATGTTCAGGTCCAAACGCTCTGGGCTGGTGCGGCGACTATGGCGAAGTCGTGTGG
  TCCCCGACCGGGAGGCAAGCGGCAACCGCCGCCGGCGGTGGCGGCTACTAGGATGGGAGC
  TTGGGCAGACGAGCTGAGCCGGCCCCGCGGCCAAGAGAGGCTGGAGCTGCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005585 unedited
  NNGGGAATTTTTNNNNNNAATCTTGNACCGCGCCGCATTCTNANGATCGNATTTTTTTTT
  TTTTTTTTTTNGAGGAAAAGAAANAAAAATATCTGTATTGAAAGAATATAAGCCAAAGTG
  CGTCTTTCTTGTTTTGTCCATATACACACATTGCACCATACATAAATAATTACATTGTAA
  AAATGACTCCATAATTACAAGTATAATATATATTTCCATATAATATATAAAACTTTATAT
  TAAATCTAGGTAGATGATATCTGGGGGGTTTTGTCCGTGGGGGCTGTGTCTCTGGGCATC
  GGCCCCTCTCGAGCCGGCAGGTGGCGGTGGCCGCGGNCTCCAACCGNCCCTCCCGCGGGG
  GCCGCACTATCTGGGTTTGTGAGGAGGATCTCCAGCCANCAGGGGCAGAGGTGATGAACT
  GCCCGGGAATACCAGGGCCCCCCAGCCCTTGGCAAACCTGATCGCCGACCCTTTGGGGTT
  CTAAGGGCCCCTCGGCGGGTTTGGGGCTTGGGGCCCTGCTTTAAGCCCAACCCTCCAAAT
  CAAACCTTTGATGGATAACCCGGGGGACCCTTTGCCCCAAAAAAGGGGCCGGCCCCCGGG
  CCCCTCCCAATTCGGGGGATTTCCAAAAAAAGGGGGCCTCCCCCCCCCTTTTGGGCCCCA
  ACCCCCCGGGGTTTTTTTTTTTAAAAAGGTGCCCAAACCCCTTTTTTTTGGGGGTTTTCC
  CCCACCAACAAATTGGGTTTTTCCAAAAGAGAAGAGCGCCCCAAAAAAAAACCCCTCCCC
  TGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005585
• Insert Size: 2400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone may be unstable or toxic at high copy number in common E. coli strain. We recommend using a lower copy number E. coli strain, such as CopyCutter strain (http://www.epibio.com/item.asp?ID=435) for transformation and plasmid preparation. Please be aware that the DNA yield could be low. Additional aliquots of this clone can be ordered from OriGene.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005585.2, NP_005576.2
• RefSeq Size: 2886 bp
• RefSeq ORF: 2886 bp
• Locus ID: 4091
• Cytogenetics: 15q22.31
• Domains: DWB, DWA, MH1
• Protein Families: Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway
• Gene Summary: 'The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants have been found for this gene.[provided by RefSeq, Sep 2014]'
  Transcript Variant: This variant (1) encodes the supported protein.