TAF9 (NM_003187) Human Untagged Clone

CAT#: SC127231

TAF9 (untagged)-Human TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa (TAF9), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TAF9
• Synonyms: MGC:5067; STAF31/32; TAF2G; TAFII-31; TAFII-32; TAFII31; TAFII32; TAFIID32
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003187, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGTCTGGCAAGACGGCTTCTCCCAAGAGCATGCCGAAAGATGCACAGATGATGGCACAAATCCTGA
  AGGATATGGGGATTACAGAATATGAGCCAAGAGTTATAAATCAGATGTTGGAGTTTGCCTTCCGATATGT
  GACCACAATTCTAGATGATGCAAAAATTTATTCAAGCCATGCTAAGAAAGCTACTGTTGATGCAGATGAT
  GTGCGATTGGCAATCCAGTGCCGCGCTGATCAGTCTTTTACCTCTCCTCCCCCAAGAGATTTTTTATTAG
  ATATTGCAAGGCAAAGAAATCAAACCCCTTTGCCATTGATCAAGCCATATTCAGGTCCTAGGTTGCCACC
  TGATAGATACTGCTTAACAGCTCCAAACTATAGGCTGAAATCTTTACAGAAAAAGGCATCAACTTCTGCG
  GGAAGAATAACAGTCCCGCGGTTAAGTGTTGGTTCAGTTACTAGCAGACCAAGTACTCCCACACTAGGCA
  CACCAACCCCACAGACCATGTCTGTTTCAACTAAAGTAGGGACTCCCATGTCCCTCACAGGTCAAAGGTT
  TACAGTACAGATGCCTACTTCTCAGTCTCCAGCTGTAAAAGCTTCAATTCCTGCAACCTCAGCAGTTCAG
  AATGTTCTGATTAATCCATCATTAATCGGGTCCAAAAACATTCTTATTACCACTAATATGATGTCATCAC
  AAAATACTGCCAATGAATCATCAAATGCATTGAAAAGAAAACGTGAAGATGATGATGATGACGATGATGA
  TGATGATGACTATGATAATCTGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003187 unedited
  NCCCCNNNNNANANNCAAAANNGNNCGGTACACATTTGTATACGACTCATATAGGCGGCC
  GCGNAATTCGCACGAGGCGCCCNGAACCGTCGCGGCGGGGACCATGTTGCTTCCGAACAT
  CCTGCTCACCGGTACACCAGGGNNGTTGGAAAAACCACACTAGGCAAAGAACTTGCGTCA
  AAATCAGGACTGAAATACATTAATGTGGGTGATTTAGCTCGAGAAGTCTGATCATCGGAT
  ATCATGGAGTCTGGCAAGACGGCTTCTCCCAAGAGCATGCCGAAAGATGCACAGATGATG
  GCACAAATCCTGAAGGATATGGGGATTACAGAATATGAGCCAAGAGTTATAAATCAGATG
  TTGGAGTTTGCCTTCCGATATGTGACCACAATTCTAGATGATGCAAAAATTTATTCAAGC
  CATGCTAAGAAAGCTACTGTTGATGCAGATGATGTGCGATTGGCAATCCAGTGCCGCGCT
  GATCAGTCTTTTACCTCTCCTCCCCCAAGAGATTNTTTATTAGATATTGCAAGGCAAAGA
  AATCAAACCCCTTTGCCATTGATCAAGCCATATTCAGGTCCTAGGTTGCCACCTGATAGA
  TACTGCTTAACAGCTCCAAACTATANGCTGAAATCTTTACAGAAAAAGGCATCAACTTCT
  GCGGNAAAGAATACAGTCCCGCGGTTAAGTGTTGGTTCAGTTACTAGCAGACCAAGTACT
  CCCACACTAGGCACACCAACCCCACAGACCATGTCTGTTTCAACTAAAGTAGGGACTNCC
  ATGTCCCCTCACAGGTCAAAAGTTTTACAGTACAGATGCCTACTTCTCAGTCTCCAGCTT
  GTAAAGCTTCAATTCCTGCAACCTCAACAGTTCAAAATGGTCTGATTATTCCTCATTATT
  CGGTTCAAAAACATCCTATTACCACTAATTGATGTCCTCCAAAATACTGGCATGATCATC
  AATGCTTGAAAAAAAACTGGANAGATGATATGCACAGATGAGATGAGCCTTTGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003187 unedited
  GTACACTATGNACCGCGCCGCAATCTATGATCGGTTTTTTTTTTTTTTTTTTGAATGAAT
  GACAACTTTATTTTTCTTACACTTTTAAGGCTGATGAAAAACCTTCATTTCAATTGAAAA
  GTATGGTAACTGTGTTTACTCATTATTATTAGTTTTCTAAAACACAACTTGAAAACATCC
  AGCATGCATGTTTAATATCAGTACAATGAATTCAAGACCAAGTATACATGTTACATTCAG
  CAAGGCTAGATTACAGATTATCATAGTCATCATCATCATCATCGTCATCATCATCATCTT
  CACGTTTTCTTTTCAATGCATTTGATGATTCATTGGCAGTATTTTGTGATGACATCATAT
  TAGTGGTAATAAGAATGTTTTTGGACCCGATTAATGATGGATTAATCAGAACATTCTGAA
  CTGCTGANGTTGCANGAATTGAAGCTTTTACAGCTGGAGACTGAGAAGTAGGCATCTGTA
  CTGTAAACCTTTGACCTGTGAGGGACATGGGAGTCCCTACTTTAGTTGAAACAGACATGG
  TCTGTGGAGTTGGTGTGCCTAGTGTGGGAGTACTTGGTCTGCTAGTAACTGAACCAACAC
  TTAACCGCGGNACTGTTTATTCTTCCCGCANAAGTTGATGCCTTTTTCTGTTAAGATTTC
  AGCCTATAGTTGGGAGCTGTTAAGCAGTATCTATCAGGTGGCACCCTANNGACCTGAATA
  TGGCCTTGATCAAATGGCAAAGGGGTTTTGATTTCTTTGCCTTTGCATATCTAATAAAAA
  AATCCTCTTGGGGGAGGAAAGTTAAAAGACTGATCAGCGCGGCACTGGTATGCCAATCGG
  ACATCATCTGCATCACAGAAGCTTTTTTAGCATGACTTGGAAAAAATTTTGCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003187
• Insert Size: 1250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003187.4, NP_003178.1
• RefSeq Size: 1245 bp
• RefSeq ORF: 795 bp
• Locus ID: 6880
• Cytogenetics: 5q13.2
• Domains: TFIID-31
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• Gene Summary: 'Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16. In human, TAF9 and AK6 (GeneID: 102157402) are two distinct genes that share 5' exons. A similar but distinct gene (TAF9L) has been found on the X chromosome and a pseudogene has been identified on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]'
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 4. Both variants 4 and 1 encode the same protein.