HLAE (HLA-E) (NM_005516) Human Untagged Clone

CAT#: SC127254

HLA (untagged)-Human major histocompatibility complex, class I, E (HLA-E)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HLA-E
• Synonyms: HLA-6.2; QA1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127254 sequence for NM_005516 edited (data generated by NextGen Sequencing)
  ATGGTAGATGGAACCCTCCTTTTACTCCTCTCGGAGGCCCTGGCCCTTACCCAGACCTGG
  GCGGGCTCCCACTCCTTGAAGTATTTCCACACTTCCGTGTCCCGGCCCGGCCGCGGGGAG
  CCCCGCTTCATCTCTGTGGGCTACGTGGACGACACCCAGTTCGTGCGCTTCGACAACGAC
  GCCGCGAGTCCGAGGATGGTGCCGCGGGCGCCGTGGATGGAGCAGGAGGGGTCAGAGTAT
  TGGGACCGGGAGACACGGAGCGCCAGGGACACCGCACAGATTTTCCGAGTGAATCTGCGG
  ACGCTGCGCGGCTACTACAATCAGAGCGAGGCCGGGTCTCACACCCTGCAGTGGATGCAT
  GGCTGCGAGCTGGGGCCCGACGGGCGCTTCCTCCGCGGGTATGAACAGTTCGCCTACGAC
  GGCAAGGATTATCTCACCCTGAATGAGGACCTGCGCTCCTGGACCGCGGTGGACACGGCG
  GCTCAGATCTCCGAGCAAAAGTCAAATGATGCCTCTGAGGCGGAGCACCAGAGAGCCTAC
  CTGGAAGACACATGCGTGGAGTGGCTCCACAAATACCTGGAGAAGGGGAAGGAGACGCTG
  CTTCACCTGGAGCCCCCAAAGACACACGTGACTCACCACCCCATCTCTGACCATGAGGCC
  ACCCTGAGGTGCTGGGCCCTGGGCTTCTACCCTGCGGAGATCACACTGACCTGGCAGCAG
  GATGGGGAGGGCCATACCCAGGACACGGAGCTCGTGGAGACCAGGCCTGCAGGGGATGGA
  ACCTTCCAGAAGTGGGCAGCTGTGGTGGTGCCTTCTGGAGAGGAGCAGAGATACACGTGC
  CATGTGCAGCATGAGGGGCTACCCGAGCCCGTCACCCTGAGATGGAAGCCGGCTTCCCAG
  CCCACCATCCCCATCGTGGGCATCATTGCTGGCCTGGTTCTCCTTGGATCTGTGGTCTCT
  GGAGCTGTGGTTGCTGCTGTGATATGGAGGAAGAAGAGCTCAGGTGGAAAAGGAGGGAGC
  TACTCTAAGGCTGAGTGGAGCGACAGTGCCCAGGGGTCTGAGTCTCACAGCTTGTAA
  
  Clone variation with respect to NM_005516.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005516 unedited
  TCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGAGGCTGGGATCATGGTAGATGGAAC
  CCTCCTTTTACTCCTCTCGGAGGCCCTGGCCCTTACCCAGACCTGGGCGGGCTCCCACTC
  CTTGAAGTATTTCCACACTTCCGTGTCCCGGCCCGGCCGCGGGGAGCCCCGCTTCATCTC
  TGTGGGCTACGTGGACGACACCCAGTTCGTGCGCTTCGACAACGACGCCGCGAGTCCGAG
  GATGGTGCCGCGGGCGCCGTGGATGGAGCAGGAGGGGTCAGAGTATTGGGACCGGGAGAC
  ACGGAGCGCCAGGGACACCGCACAGATTTTCCGAGTGAATCTGCGGACGCTGCGCGGCTA
  CTACAATCAGAGCGAGGCCGGGTCTCACACCCTGCAGTGGATGCATGGCTGCGAGCTGGG
  GCCCGACGGGCGCTTCCTCCGCGGGTATGAACAGTTCGCCTACGACGGCAAGGATTATCT
  CACCCTGAATGAGGACCTGCGCTCCTGGACCGCGGTGGACACGGCGGCTCAGATCTCCGA
  GCANAAGTCANATGATGCCTCTGAGGCGGAGCACCAGAGAGCCTACCTGGAAGACACATG
  CGTGGAGTGGGCTCACAATACCTGGAGAAGGGAAGGAGACGCTGCTTCACCTGGAGCCCC
  CAAAGACACACGTGACTCACCCCCCATCTCTGACATGAGGCCACCCTGAGTGCTGGCCCC
  TGGCTTNCTACCTGCGAGATACACTGACCTGCCACAGATGGGGAGGCCATACCAGACACG
  AGCTCGTGAGACAGCCTGCAGGGATGGACCTTCANAGTGGCACTGGGTGGTGCCTCTTGA
  AGAACNAGATCCGTGCATTGCACATGAGGGCTCCCGAGCCTACCTGAATGGAGCGTTCCA
  GCACATCCATGGGGATATGCTGCTGGTNNCTGATCGGCCTGACTGGTGTGTGGATGAGAA
  AAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005516 unedited
  CTATGGCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATTTTTATTTT
  TTGAGATGGCGTTTCACCCTGTCACCCAGGCTGGAGTGCAGTCGCACAATCCTGGCTCAC
  TGCAACCTCCGCCTCCCGGGTTGACGCCATTCTCCTGCCTCCGCCTCCTGAGTAGCTGGG
  ACTACAGGCCCGTGCCTCTGCGCCCAGCTAATTTTTTGTATTTTTTAGTAGAGACGGGGT
  TTCACCGTGTTAGCCAGGATGGTTTCGATCTCCTGACCTCGTGATCTGCCCTCCTCGGCC
  TCCCTAAGTGCTGGGATTACAGGCATGAGCTGCCGCACTCTGCCCAGGTTCTTATTTTTA
  ATAGAAAAGAGAACAGGGAAGGAACACAGGTCAGTGTGAGGAAGGGGGTCATGGTATACA
  CAGAGGTGGACTGTTTCTCTACCTCCTCACATTGTGCTAACAGGGACACAGACAGATTCA
  GAGGCCCTTGCAAAAAGAGAAGCCAGAGTCCCCTAAGACACATAGGGGAGGCGTGAGGAA
  ATCCTGCATCTCAGTCGCACACAAGGCAGCTGTGCATCTCAGTCGCACACAAGGCAGCTG
  TCTCAGGCTTTACAAGCTGTGAGACTCAGACCCCTGGGCACTGTCGCTCCACTCAGCCTT
  AGAGTACCTCCCTCCTTTTCCACCTGAGCTCTTTTTCCTCCATATCACAGCAGCCACCAC
  AGCTCCAGAGACCACAGATCCAAGGAGAACCAGCCAGCAATGATGCCCACGATGGGGTAT
  GTGGGCCTGGAAACCCGCTTCCATCTCAAGTGACGGCCTCGGGGTACCCCTCATGTTGAC
  ATGCACGTGTATCTCTGCTTCTTTCAGAGGGCCCACACAACTGCCCATTTTGGAAGTTCA
  TCCCTGAAGCCTGTTTCCAGACTCGGGCCGGGTATGCCCTCCATCTGCTGCAGCCATGGA
  ATCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005516
• Insert Size: 1780 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005516.4, NP_005507.3
• RefSeq Size: 1701 bp
• RefSeq ORF: 1077 bp
• Locus ID: 3133
• Cytogenetics: 6p22.1
• Domains: MHC_I, ig, IGc1
• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Endocytosis, Graft-versus-host disease, Natural killer cell mediated cytotoxicity, Type I diabetes mellitus, Viral myocarditis
• Gene Summary: 'HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq, Jul 2008]'