USP4 (NM_003363) Human Untagged Clone

CAT#: SC127280

USP4 (untagged)-Human ubiquitin specific peptidase 4 (proto-oncogene) (USP4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: USP4
• Synonyms: UNP; Unph
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003363, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGAAGGTGGAGGCTGCCGTGAGCGACCGGATGCGGAGACTCAGAAGTCCGAGCTTGGACCCTTAA
  TGAGGACCACACTCCAACGCGGGGCGCAGTGGTATCTTATTGACAGCCGGTGGTTCAAGCAGTGGAAGAA
  GTATGTGGGCTTTGACAGCTGGGACATGTACAATGTGGGTGAACATAACCTATTTCCTGGCCCAATAGAC
  AACTCTGGGCTATTTTCAGATCCTGAGAGTCAGACCTTGAAAGAACACTTAATTGATGAATTGGACTATG
  TATTGGTCCCTACCGAGGCGTGGAATAAACTACTAAACTGGTACGGCTGTGTAGAAGGCCAGCAACCCAT
  CGTCAGAAAAGTTGTGGAGCATGGCCTGTTTGTCAAGCACTGCAAAGTCGAGGTGTATTTGCTGGAACTG
  AAGCTCTGTGAGAACAGTGACCCCACCAATGTGCTGAGTTGCCATTTCAGCAAGGCAGACACCATTGCAA
  CCATCGAGAAAGAGATGCGGAAGCTATTCAACATCCCTGCGGAGCGTGAAACACGGCTCTGGAACAAATA
  CATGAGCAACACCTACGAGCAGTTGAGCAAGCTAGACAACACTGTCCAGGATGCTGGGCTATACCAGGGT
  CAGGTGCTAGTAATTGAGCCTCAAAATGAAGATGGCACATGGCCCAGGCAGACCTTGCAGTCAAAATCAA
  GCACTGCGCCTAGCAGAAATTTTACTACCTCTCCAAAATCATCAGCAAGTCCCTATTCCTCAGTGTCTGC
  CTCTCTCATTGCAAATGGTGATAGCACTAGCACCTGTGGGATGCACAGTTCCGGTGTCAGCAGGGGTGGA
  TCTGGCTTTTCTGCTTCGTATAATTGTCAGGAGCCACCATCCTCTCATATACAACCTGGGCTCTGTGGAC
  TTGGAAACCTGGGAAACACCTGCTTCATGAACTCCGCTTTGCAGTGTTTGAGCAACACTGCACCACTGAC
  TGACTACTTTCTCAAAGATGAGTATGAAGCCGAAATCAACAGAGACAACCCTCTGGGGATGAAAGGGGAA
  ATTGCAGAAGCCTATGCTGAACTCATTAAGCAGATGTGGTCTGGAAGGGACGCCCATGTGGCACCTCGCA
  TGTTCAAAACTCAAGTAGGACGTTTTGCTCCTCAATTTTCTGGCTACCAGCAACAAGATTCTCAGGAGCT
  GCTGGCCTTTCTTCTAGATGGATTGCATGAAGATCTGAACCGGGTAAAGAAAAAGCCCTACTTGGAGCTG
  AAGGATGCCAATGGGCGGCCAGATGCGGTGGTGGCAAAGGAAGCCTGGGAGAATCACAGGTTGAGGAATG
  ATTCTGTGATTGTGGATACTTTCCATGGCCTCTTCAAATCTACTTTGGTTTGCCCAGAATGTGCTAAGGT
  TTCTGTGACCTTTGACCCATTTTGCTATCTAACGCTGCCACTGCCCTTGAAGAAAGATCGAGTTATGGAG
  GTTTTCCTGGTTCCTGCTGACCCTCACTGCAGACCTACTCAGTACCGTGTGACTGTGCCGCTGATGGGGG
  CTGTGTCCGACCTGTGCGAGGCTCTCTCCAGGCTGTCTGGCATTGCTGCAGAAAATATGGTGGTCGCAGA
  TGTGTATAATCACCGATTCCACAAAATTTTCCAAATGGATGAAGGTTTAAACCACATCATGCCTCGGGAT
  GACATTTTCGTGTACGAGGTCTGCAGCACTTCCGTGGATGGCTCGGAATGTGTCACGCTTCCAGTCTACT
  TCAGGGAGAGGAAGTCCAGGCCATCAAGCACTTCCTCCGCATCAGCGCTATATGGGCAGCCACTATTGCT
  TTCTGTCCCCAAGCACAAGTTAACCCTTGAGTCTTTGTACCAGGCTGTTTGTGATCGTATCAGCCGCTAT
  GTGAAACAGCCTTTACCTGATGAGTTTGGCAGCTCACCCTTGGAGCCAGGGGCCTGCAATGGCTCCAGGA
  ACAGCTGTGAAGGAGAAGATGAGGAAGAAATGGAGCATCAGGAAGAAGGCAAAGAGCAGCTTTCAGAAAC
  AGAAGGCAGTGGGGAAGATGAGCCAGGAAATGACCCCAGTGAGACCACCCAAAAGAAGATCAAAGGCCAG
  CCCTGCCCAAAAAGGCTTTTTACCTTCAGTCTTGTGAACTCCTATGGAACAGCTGACATAAATTCACTTG
  CAGCTGATGGAAAACTACTTAAACTCAACTCTCGATCTACACTGGCCATGGATTGGGACAGTGAAACTCG
  GAGACTTTACTATGATGAGCAAGAATCTGAGGCCTACGAGAAGCATGTGAGCATGTTGCAGCCTCAGAAG
  AAGAAGAAGACCACAGTGGCCCTGAGAGACTGCATCGAGCTCTTCACCACCATGGAGACCCTTGGGGAGC
  ATGACCCCTGGTACTGTCCCAACTGTAAGAAGCATCAACAGGCCACAAAAAAGTTTGACCTATGGTCCTT
  GCCCAAGATCCTGGTGGTCCACCTCAAACGTTTCTCCTACAACAGATACTGGAGGGATAAGCTCGACACA
  GTCGTAGAATTCCCAATCAGAGGGCTGAACATGTCCGAGTTTGTCTGTAACCTGTCAGCAAGGCCTTATG
  TGTACGACCTCATTGCCGTGTCCAATCATTATGGAGCCATGGGGGTTGGCCACTACACTGCATATGCGAA
  GAACAAACTGAATGGTAAATGGTATTACTTTGATGATAGCAACGTGTCCCTGGCCTCTGAGGATCAGATA
  GTGACTAAAGCAGCTTATGTGCTATTTTACCAACGTCGAGATGATGAATTTTATAAGACACCTTCACTTA
  GCAGTTCTGGTTCCTCTGATGGAGGGACACGACCAAGCAGCTCTCAGCAGGGCTTTGGGGATGATGAGGC
  TTGCAGCATGGACACCAACTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003363 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGATGGCGGAAGGTGGA
  GGCTGCCGTGAGCGACCGGATGCGGAGACTCAGAAGTCCGAGCTTGGACCCTTAATGAGG
  ACCACACTCCAACGCGGGGCGCAGTGGTATCTTATTGACAGCCGGTGGTTCAAGCAGTGG
  AAGAAGTATGTGGGCTTTGACAGCTGGGACATGTACAATGTGGGTGAACATAACCTATTT
  CCTGGCCCAATAGACAACTCTGGGCTATTTTCAGATCCTGAGAGTCAGACCTTGAAAGAA
  CACTTAATTGATGAATTGGACTATGTATTGGTCCCTACCGAGGCGTGGAATAAACTACTA
  AACTGGTACGGCTGTGTAGAAGGCCAGCAACCCATCGTCAGAAAAGTTGTGGAGCATGGC
  CTGTTTGTCAAGCACTGCAAAGTCGAGGTGTATTTGCTGGAACTGAAGCTCTGTGAGAAC
  AGTGACCCCACCAATGTGCTGAGTTGCCATTTCAGCAAGGCAGACACCATTGCAACCATC
  GAGAAAGAGATGCGGAAGCTATTCAACATCCCTGCGGAGCGTGAAACACGGCTCTGGAAC
  AAATACATGAGCAACACCTACGAGCAGTTGAGCAAACTAGACAACACTGTCCAGNATGCT
  GGGCTATACCAGGGTCAGGTGCTAGTAATTGAGCCTCANAATGAAGATGGCACATGGGCC
  CAGCAGACCTTGCAGTCAAAATCAAGCACTGCGCCTAGCAGAAATTNTACTACCTCTCCA
  AAATCATCAGCAAGTCCCTATTTCTCAGTGTCTGCCTCTCTCATTGCANATGGTGATAGC
  ACTAGCACCTGTGGGATGCACAGTTNCCGGTGTCAGCAGGGGTGGGATCTGGCTNTTCTG
  CTTCGNATAATGGCAGGAGCACCATNCTCTCTATACAACTGGGCTCTGTGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003363 unedited
  GGGAGCAGGCCACCCCGAAAACTTNNNANCNGNTTTATTGNACCGGGCCGCATTCTANAT
  CGAGTTTTTTTTTTTTTTTTTTGTTTCCTTCTGCTCATAAAAGAAGGTATTTCCTTGTCT
  GGTTTTTAGACAGAACACTAGCAGTCTGCAGAGTGTCAAAGATGTTCTCCTGGGGGATTA
  CACTGGCGCTACAGGGTGGCAGGATCGTGGAGTCAGCATTAGTTGGTGTCCATGCTGCAA
  GCCTCATCATCCCCAAAGCCCTGCTGAGAGCTGCTTGGTCGTGTCCCTCCATCAGAGGAA
  CCAGAACTGCTAAGTGAAGGTGTCTTATAAAATTCATCATCTCGACGTTGGTAAAATAGC
  ACATAAGCTGCTTTAGTAGTGGCCAACCCCCATGGCTCCATAATGATTGGACACGGCAAT
  GAGGTCGTACACATAAGGCCTTGCTGACAGGTTACAGACAAACTCGGACATGTTCAGCCC
  TCTGATTGGGAATTCTACGACTGTGTCGAGCTTATCCCTCCAGTATCTGTTGTAGGAGAA
  ACGTTTGAGGTGGACCACCAGGATCTTGGGCAAGGACCATAGGTCAAACTTTTTTGTGGC
  CTGTTGATGCTTCTTACAGTTGGGACAGTACCAGGGGTCATGCTCCCCAAGGGTCTCCAT
  GGTGGTGAAGAGCTCGATGCAGTCTCTCAGGCCCACTGTGGTTCTTCTTCTTCTTCTGAA
  GCTGGCACATGCTCACATGCTTCTCGTAGGCCTCAGATTCTTGCTCATCATAGTAAAAGT
  CTCCGAGTTTCACTGGTCCCAATCCATGGCCCAGTGTAAATACGAAAGNTTGAGTTTAAA
  GTAGTTTTCCCTTCAGCTGCAAGTGGAAATTAATGTCAGCCTGGTTCCATTAGAAGTTCA
  CCAAGACTGGAAGGTAAAAAAGCCTTTTTGGGGCACGGCTTGGCCTTCTGATCTTCCTTT
  TGGGNGGCNCCTCCTGGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003363
• Insert Size: 2920 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003363.2, NP_003354.2
• RefSeq Size: 3696 bp
• RefSeq ORF: 2892 bp
• Locus ID: 7375
• Cytogenetics: 3p21.31
• Domains: UCH, DUSP
• Protein Families: Druggable Genome, Protease
• Gene Summary: 'The protein encoded by this gene is a protease that deubiquitinates target proteins such as ADORA2A and TRIM21. The encoded protein shuttles between the nucleus and cytoplasm and is involved in maintaining operational fidelity in the endoplasmic reticulum. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).