UBE2H (NM_182697) Human Untagged Clone

CAT#: SC127291

UBE2H (untagged)-Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBE2H
• Synonyms: E2-20K; GID3; UBC8; UBCH; UBCH2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127291 sequence for NM_182697 edited (data generated by NextGen Sequencing)
  ATGTCATCTCCCAGTCCGGGCAAGAGGCGGATGGACACGGACGTGGTCAAGCTCATCGAG
  AGTAAACATGAGGTTACGATCCTGGGAGGACTTAATGAATTTGTAGTGAAGTTTTATGGA
  CCACAAGGAACACCATATGAAGGCGGAGTATGGAAAGTTAGAGTGGACCTACCTGATAAA
  TACCCTTTCAAATCTCCATCTATAGATCTTACCAATATATTTGAGTCCTTCCTGCCTCAG
  TTATTGGCCTATCCTAACCCCATAGATCCTCTCAATGGTGACGCTGCAGCCATGTACCTC
  CACCGACCAGAAGAATACAAGCAGAAAATTAAAGAGTACATCCAGAAATACGCCACGGAG
  GAGGCGCTGAAAGAACAGGAAGAGGGTACCGGGGACAGCTCATCGGAGAGCTCTATGTCT
  GACTTTTCCGAAGATGAGGCCCAGGATATGGAGTTGTAG
  
  Clone variation with respect to NM_182697.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_182697 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAGGCCGACTGAG
  CAGGAGTCGTCCGCTTATAGTGGAGGCTGCTAGGAGCCGGTCAGAGGGTGAGTGGGGAGC
  AGGCGAGCGAGCCACAGCAGGCAGGAGGGAGCGTCGCGCGGCGCAGAGGAGCGCCGGGGC
  ATGGCCGCGGCGGAACCACAGCCGGAGAGGGGCAGCCCGAGCCGGGCGCCGCAGGGTCCC
  CACCCCCACCCGGCCGGACAGTGCCCGGTGTTCCCCCGTGCGGGGGGCGGCGGCGGCGGC
  TGACGGGACCGCCGGGACCGCGGGGGTGTTGCCACCTCCACCGAGGAGCCGGCGCGGTCG
  CGGGCTCCTCAGAGCCGGGGCCCCGGGCCCGTGACAGACGGTCCGAGGAATGGAGAGAGG
  CGGCGGCGACACCATGTCATCTCCCAGTCCGGGCAAGAGGCGGATGGACACGGACGTGGT
  CAAGCTCATCGAGAGTAAACATGAGGTTACGATCCTGGGAGGACTTAATGAATTTGTAGT
  GAAGTTTTATGGACCACAACGAACACCATATGAAGGCGGAGTATGGAAAGTTAGAGTGGA
  CCTACCTGATAAATACCCTTTCAAATCTCCATCTATAGGATTCATGAATAAAATTTTCCA
  TCCCAACATTGATGAAGCGTCACGAACTGCGTGTCTAGATGTAATTAATCAAACTTGGAC
  AGCTCTCTATGATCTTACCAATATATTTGAGTGCTTGCTGCCTCAGTTATTGGCCTATCC
  TAACCCCATAGATCCTCTGCATGGATGACGCTGCAGGCATGTACCTTCACCGACCAGATG
  AATACAGCNGAATATGAAGAGTGCATTCAGAATTACGCACNGGAGGATGCGCTGCAAGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_182697 unedited
  ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTAAAAAAAACCTTTTGC
  TTCATGCCCGTAATTAACATAAAGTAAGTAAATTCTTTGTCTTTTTATTTAGGAAAATAA
  TCATGCTAAAAGCAAGTTGTACTTTATATAGATTTTCAAACAAAAGATTGATTGGGCTTT
  TTGAATAAAAAAGATAGGGTATCTTCCAACCTCACTGACTTAAACCTTGTGGAATACAGC
  ACTTCTAAACTAAAAACACACGTCAAAAAGAAAAATTAAATAATCCCAGTGGGATGCTCA
  AAAATCGTCTTTAGAAGGTTTTAGGATTGGCTAATTCTGCTATTATCTTATTGGCTGCGG
  CAAAAAAAGAACACAGAGCTGCTGTCTAAAATTTCTCCAAACTCCCCTCCGAGTTCAGCA
  GGCAACACTCAGATTTGAAAGAAACTCTGCAGCAACCTGGACATATGGTTAGCTGAGTCG
  ACATTTGATTAGCAGGCATTTTAAACTGATTTCCTCTTAACATACGCAGGAGAGAAAACA
  TTTTCTTCCTGCAGGGCATGTTCTACTACATCATATTTCTGAATCCCTTCTTCCTATTCG
  GTCACTCTCTGAAAAAAGCTGGTTTGTTTTTCCCATGTAGAATCACTCTGGGTACAAGAA
  CCTTTATTTTGTCCCATTATTATTTGTTTTAATNTAGAACCCTGTGTTGGGTCTAANATA
  CCCATTTCCCACTCCCACCAGCAGATGATGACCAAGCTGCTGCCCAGAGCAGGAGAAAAA
  GGGAAAACCTAACCATAACTGTAGGCCAGTACCCAGGCCTTTGCATTCCCACGTGGGTCG
  CCTCCTAAGTTTAATGCTCATGACCGCTTGTCCACGAAGCGGCCGCCTTTGGTAGGGAAT
  ATAAATCTGAACCTTTTTAAACAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_182697
• Insert Size: 2820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182697.1, NP_874356.1
• RefSeq Size: 2635 bp
• RefSeq ORF: 459 bp
• Locus ID: 7328
• Cytogenetics: 7q32.2
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: 'The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011]'
  Transcript Variant: This variant (2) lacks two consecutive in-frame exons in the coding region, as compared to variant 1. The resulting isoform (2) lacks an internal segment, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.