IBTK (NM_015525) Human Untagged Clone

CAT#: SC127308

IBTK (untagged)-Human inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IBTK
• Synonyms: BTBD26; BTKI
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015525, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGTTCACCCATGCCTGACTGCACATCAAAGTGTCGATCCCTGAAGCATGCTTTGGATGTCCTTTCTG
  TGGTAACAAAGGGGAGCGAAAACCAGATTAAGGCCTTTCTCTCCAGTCATTGTTACAATGCTGCAACTAT
  CAAGGATGTTTTTGGCAGGAATGCCCTCCACCTTGTTTCCTCCTGTGGAAAGAAAGGAGTGTTAGATTGG
  CTTATTCAGAAAGGAGTGGATCTGTTGGTGAAAGACAAAGAGTCTGGATGGACAGCATTGCACAGAAGCA
  TTTTTTATGGACATATTGATTGTGTTTGGTCTCTATTGAAGCATGGTGTTAGTCTGTATATTCAAGATAA
  AGAAGGCTTGTCAGCTTTGGATCTTGTAATGAAGGATAGACCAACTCATGTAGTATTCAAGAATACTGAT
  CCTACAGATGTTTATACTTGGGGCGATAATACAAATTTTACCCTGGGTCATGGAAGCCAGAATAGCAAAC
  ATCATCCAGAGTTGGTGGATCTGTTCTCCAGGAGTGGGATTTATATCAAGCAGGTGGTGCTTTGTAAATT
  TCACTCCGTGTTTCTGTCTCAGAAAGGGCAGGTTTATACCTGTGGTCATGGTCCTGGAGGGCGATTAGGA
  CATGGAGATGAACAGACATGCTTGGTCCCTCGGCTTGTGGAAGGACTGAATGGTCATAATTGTTCCCAAG
  TGGCAGCTGCTAAGGATCATACTGTTGTATTAACTGAAGATGGATGTGTTTATACATTTGGTCTAAACAT
  TTTTCATCAATTAGGAATTATTCCACCGCCTTCCAGTTGTAATGTACCCAGACAGATACAGGCAAAATAT
  CTGAAAGGAAGGACAATCATTGGCGTTGCAGCAGGCAGGTTTCATACAGTCCTATGGACTAGAGAAGCTG
  TTTACACTATGGGACTAAATGGTGGACAACTGGGTTGTTTGCTAGATCCCAATGGAGAAAAGTGTGTAAC
  TGCTCCTCGTCAGGTCTCTGCCCTTCACCATAAAGACATTGCTCTGTCTTTGGTTGCTGCAAGTGATGGA
  GCTACAGTCTGTGTTACCACAAGGGGAGATATTTACTTACTTGCAGACTATCAGTGCAAGAAGATGGCTT
  CTAAACAGTTGAACTTGAAAAAAGTTCTTGTGTCTGGGGGTCATATGGAATACAAGGTTGATCCTGAACA
  TTTGAAAGAAAATGGGGGTCAAAAAATTTGCATTCTTGCAATGGATGGAGCTGGAAGGGTGTTTTGCTGG
  AGATCAGTCAACAGTTCTCTGAAGCAGTGTCGATGGGCCTATCCACGTCAGGTCTTCATTTCTGATATTG
  CTTTAAATAGAAATGAAATTCTATTTGTTACGCAAGATGGAGAAGGATTTAGAGGGAGATGGTTTGAAGA
  GAAAAGAAAGAGTTCTGAAAAGAAAGAGATTTTATCAAACCTTCACAATTCCTCATCAGATGTGTCTTAT
  GTCTCTGATATAAATAGTGTGTATGAAAGAATTCGACTTGAGAAACTTACCTTTGCACATAGAGCTGTTA
  GTGTCAGCACAGATCCAAGTGGATGCAACTTTGCAATCCTGCAGTCAGATCCTAAAACAAGCCTTTATGA
  AATTCCAGCTGTGTCCTCATCATCCTTTTTTGAAGAGTTTGGCAAACTGTTGAGGGAAGCAGATGAAATG
  GACAGCATTCATGATGTGACATTTCAAGTTGGCAATAGACTCTTCCCTGCACATAAATATATTTTGGCAG
  TGCATTCTGATTTTTTTCAGAAATTGTTTCTTTCAGATGGTAATACTTCAGAATTTACAGATATTTACCA
  GAAAGATGAAGATTCTGCAGGGTGCCATCTCTTTGTGGTAGAGAAGGTTCATCCTGACATGTTTGAATAC
  CTTTTACAATTTATATACACAGATACTTGTGACTTTTTAACTCATGGCTTCAAACCAAGAATACACTTAA
  ACAAAAACCCAGAAGAATATCAGGGAACTCTGAATTCTCATTTGAATAAAGTGAATTTCCATGAAGATGA
  TAACCAGAAGTCTGCATTTGAAGTTTACAAAAGTAATCAAGCTCAAACAGTTAGTGAGAGGCAGAAGAGC
  AAACCTAAATCTTGTAAAAAAGGAAAAAATATTAGGGAAGATGATCCTGTAAGAATGTTGCAAACTGTTG
  CAAAGAAATTCGACTTCAGTAATTTGAGTAGTAGGTTAGATGGAGTCAGATTTGAAAATGAAAAAATTAA
  TGTTATTGCCAAGAACACTGGTAATAAACTGAAGCTAAGTCAGAAAAAATGTTCATTTCTGTGTGACGTG
  ACCATGAAATCAGTGGATGGAAAGGAATTTCCTTGTCATAAATGTGTTCTTTGTGCTAGACTTGAATATT
  TTCATAGTATGCTGAGTAGCTCATGGATTGAGGCTTCCAGTTGTGCAGCTCTGGAAATGCCAATACATTC
  TGATATATTAAAAGTTATTTTGGACTACCTCTATACTGATGAAGCTGTGGTGATAAAAGAATCTCAAAAT
  GTAGATTTTATTTGTAGTGTTCTTGTGGTGGCTGATCAACTTCTCATAACCCGGTTGAAAGAGATTTGTG
  AAGTAGCATTAACTGAAAAACTTACCCTGAAGAATGCTGCTATGCTACTGGAATTTGCAGCAATGTATAG
  TGCAAAACAGTTGAAACTGTCTTGTTTACAGTTTATAGGATTGAATATGGCAGCTTTACTTGAAGCAAGG
  TCTCTTGATGTTTTAAGCGATGGTGTTTTGAAGGATCTTTCTGAGTTTTACCGGAAAATGATTCCAGCAA
  TGGATAGAAGAGTCATTACACCATATCAAGATGGACCAGATATTAGCTATTTGGAAGTAGAAGATGGAGA
  TATCTTCTTGAAAGAAGAAATAAATATGGAACAAAATCATTCGGAAACTATGTTCAAGAAAGCAAAAACA
  AAAGCTAAAAAGAAGCCACGTAAACGTTCAGATAGTTCTGGAGGTTATAACCTTTCAGATATTATTCAGA
  GTCCATCATCTACAGGATTATTAAAGTCTGGTAAGACCAATTCTGTGGAATCTCTTCCAGAACTGTTGAC
  ATCAGACTCTGAAGGAAGCTATGCAGGAGTGGGTAGTCCTAGAGATTTACAGTCCCCTGATTTCACAACA
  GGATTTCATTCAGATAAGATTGAGGCGAAAGTCAAACCGTATGTTAATGGTACATCTCCTGTGTATTCTA
  GGGAAGATTTAAAACCATGGGAAAAGTCACCAATACTTAAAATATCTGCTCCACAGCCTATTCCCAGTAA
  CAGAATTGATACTACCAGCTCTGCCAGTTGGGTTGCTGGTTCTTTCAGTCCTGTCAGCCCTCCTGTTGTG
  GATCTCAGAACTATCATGGAAATAGAAGAAAGTAGACAAAAATGTGGAGCTACACCAAAGTCACATTTAG
  GCAAAACAGTTTCTCATGGAGTTAAACTTTCTCAGAAGCAACGAAAAATGATTGCATTGACTACCAAGGA
  AAACAATTCAGGAATGAATAGCATGGAAACAGTTTTATTCACTCCTTCAAAAGCCCCCAAACCAGTGAAT
  GCATGGGCATCTTCTCTGCATTCAGTTTCATCCAAGTCATTCCGGGATTTCTTACTAGAAGAAAAAAAGT
  CTGTTACTAGCCATAGTTCAGGCGATCATGTCAAAAAAGTTTCTTTTAAAGGAATTGAAAATTCTCAGGC
  ACCAAAAATTGTCAGATGCTCTACCCATGGTACCCCAGGACCAGAAGGCAACCATATTTCAGATTTACCA
  CTTCTAGACAGTCCCAATCCCTGGCTATCTTCTTCAGTGACTGCTCCATCCATGGTAGCCCCAGTCACTT
  TTGCATCTATTGTAGAAGAAGAACTACAACAAGAAGCAGCTCTTATTAGAAGTCGAGAAAAACCGTTGGC
  TCTGATTCAGATTGAGGAGCATGCCATACAAGATTTATTGGTTTTCTATGAGGCATTTGGCAACCCTGAA
  GAGTTTGTCATTGTTGAAAGGACACCGCAGGGACCACTGGCAGTACCTATGTGGAATAAGCATGGATGCT
  AG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015525 unedited
  GTATACGACTCCTATAGGCGGCCGCGAATTCGGCACGAGGCTGTATTGCAGCCGCCTGCC
  GAACTGACCCGGGTCTGGGGACTGGCCCCTCTGGCGCCGTTCGGTTTCTCTTATTGCCTT
  CACTGAGGATGAGTCCCTTTGTGGCTCTATGTGGACCCTGCGGAATCCACCGGCGCAGTT
  TCATCTAGCGACTGGTCACCCTTGCAATTATGGATATTTAAAAGGGTCAGACATGTGGAT
  GAGGGGGAGTTCCCCTTCTCACTCCCCCTTGGTGCTTGACTCCAGGAATAATATATAAAC
  TGTGGAATTTTTTTAAATGAAGAACTTGTATTTGATATGAACTGTATAGAGATATTTATA
  ATTTTTTTGATTTAAGTGCCAAAAAATTGTATAAAGATATATAGTTTTATACTATTGTCA
  GGAGGATTTAAATTATCCTAAAAAGTCATTGATACAATGCTGCAACTATCAAGGATGTTT
  TTGGAAAGAATGCCCTCCACCTTGTTTACTCCTGAGAAAAGAAAGGAATGTTAAATTGGC
  TTATTCATAAAGGAGTGGATCTGTTGGAGAAAGACAAAGAGTCTGGATGGACAGCATTGC
  ACAGAAACATTTTTTATGGACATATTGAAAGCGTTTGGTATCTATTGAAGCATGGTGGTA
  GTCTGTATATTCAAGATAAAGAAGGCTTGTCAACTTTAGATCTTGTGATGAACGATAGAC
  CAACTCATGTAGTAATGAAGAATACTGATACTACAGATGTTTATACTTGGGGCGATAATA
  CAAATAATACCCTGCGTCATGGGAAGCCACATAGCAAACATCATGCAGAGTTGGTGGATC
  TGTTCTGCAGCAGTGAGATCATATCAAGCAGTTGCGCTTTGTAATTTCACTCAGTGTTCT
  GTCTTAAAAAGGCAGTTATACCTGGGTAATGGACCGAAAGCAATTAGACATGAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for XM_041401 unedited
  GCGGCCGTATTTTATTGTCGAGTTTTTTTTTTTTTTTTTTTTGAAAGGATCAAGATCATT
  TTTTATTGCACATCTGCTTACATGTCTGCTGTTTTTGGGCAGAGTTAAACCTGACATATT
  GAGCCAGTTTCCGTCATTTTACATCAACTAAATTATACCACCGAACCGCCCCCGCGTCTC
  CCAGGCTTATTTTTTATAAACTAAANAATTAACCTTTTCAATTTATCTAGGCCCTGAAGC
  ACAAATTAAACATTTGTTCTTGCCAAATTTAAATACTTAGAAGAAAAGCAAACCACATCC
  TATACAGAAAACAACTTTTATGTCCAGATATACAATTGACGCCCAGAGAACTCCTGATTA
  TACAGTATTGTAAAGGGACCGCCAATCCCCACCCTTTTTCAAACCATCCAACTCCGGCCT
  ATGCATTCATCCTTCATTTTCCCAAACCTTACCCTTTTCGCGATTGATTTTTTCACATTA
  GGTATTGTTAACATTGCTCCACCCACCAAAAATAACCCACATTGACTCCCACCCCGCCCC
  CCCCTTAAAAATGTTTGTTTTGGGTATAAACCGGCATAATCTCCGTCCCCTTACCGCTCG
  CACGGCCCCTCGGTTCACGCGAAAGGAAATTTTGGGTTATAATATCCGGGGTCGCGTCCC
  CCCCGCTTCCCACACATTTTCCCCTCCCCCACCCTCGGTTTAATACCACTCAACCGCCTT
  TCTTTTTTTTATTCGTCACAAGACCCCCGCCCCCCCCCGCCCCCCATCCGTGCTTTTCTC
  TACTCCCCCCCCCTCCCCCCCTGTTCGTCTTCTTTCCTCGAAAAATTTATACCTCACCCC
  GCACCCCCTTCCTTTTCTCCCCCCACCTCCCCCGTCCCCCCTTCCCCTCCTCCTCCTTTC
  CCCCTCACATCCCGATCCAACGTTATTTTTTTTTATTTATTTTTTAGCCAACTTACCCCC
  CCCCCTCCCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015525
• ORF Size: 4062 bp
• Insert Size: 5000
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_015525.2, NP_056340.2
• RefSeq Size: 5775
• RefSeq ORF: 4062
• Locus ID: 25998
• Protein Families: Druggable Genome
• Gene Summary: Bruton tyrosine kinase (BTK) is a protein tyrosine kinase that is expressed in B cells, macrophages, and neutrophils. The protein encoded by this gene binds to BTK and downregulates BTK's kinase activity. In addition, the encoded protein disrupts BTK-mediated calcium mobilization and negatively regulates the activation of nuclear factor-kappa-B-driven transcription. This gene has a pseudogene on chromosome 18. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).