Aquaporin 4 (AQP4) (NM_004028) Human Untagged Clone

CAT#: SC127332

AQP4 (untagged)-Human aquaporin 4 (AQP4), transcript variant b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AQP4
• Synonyms: MIWC; WCH4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127332 sequence for NM_004028 edited (data generated by NextGen Sequencing)
  
  
  ATGGTGGCTTTCAAAGGGGTCTGGACTCAAGCTTTCTGGAAAGCAGTCACAGCGGAATTTCTGGCCATGC
  TTATTTTTGTTCTCCTCAGCCTGGGATCCACCATCAACTGGGGTGGAACAGAAAAGCCTTTACCGGTCGA
  CATGGTTCTCATCTCCCTTTGCTTTGGACTCAGCATTGCAACCATGGTGCAGTGCTTTGGCCATATCAGC
  GGTGGCCACATCAACCCTGCAGTGACTGTGGCCATGGTGTGCACCAGGAAGATCAGCATCGCCAAGTCTG
  TCTTCTACATCGCAGCCCAGTGCCTGGGGGCCATCATTGGAGCAGGAATCCTCTATCTGGTCACACCTCC
  CAGTGTGGTGGGAGGCCTGGGAGTCACCATGGTTCATGGAAATCTTACCGCTGGTCATGGTCTCCTGGTT
  GAGTTGATAATCACATTTCAATTGGTGTTTACTATCTTTGCCAGCTGTGATTCCAAACGGACTGATGTCA
  CTGGCTCAATAGCTTTAGCAATTGGATTTTCTGTTGCAATTGGACATTTATTTGCAATCAATTATACTGG
  TGCCAGCATGAATCCCGCCCGATCCTTTGGACCTGCAGTTATCATGGGAAATTGGGAAAACCATTGGATA
  TATTGGGTTGGGCCCATCATAGGAGCTGTCCTCGCTGGTGGCCTTTATGAGTATGTCTTCTGTCCAGATG
  TTGAATTCAAACGTCGTTTTAAAGAAGCCTTCAGCAAAGCTGCCCAGCAAACAAAAGGAAGCTACATGGA
  GGTGGAGGACAACAGGAGTCAGGTAGAGACGGATGACCTGATTCTAAAACCTGGAGTGGTGCATGTGATT
  GACGTTGACCGGGGAGAGGAGAAGAAGGGGAAAGACCAATCTGGAGAGGTATTGTCTTCAGTATGA
  
  Clone variation with respect to NM_004028.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004028 unedited
  ACTATAGGCGGCCGCGACATTCGCACGAGGGAAGGCATGAGTGACAGACCCACAGCAAGG
  CGGTGGGGTAAGTGTGGACCTTTGTGTACCAGAGAGAACATCATGGTGGCTTTCAAAGGG
  GTCTGGACTCAAGCTTTCTGGAAAGCAGTCACAGCGGAATTTCTGGCCATGCTTATTTTT
  GTTCTCCTCAGCCTGGGATCCACCATCAACTGGGGTGGAACAGAAAAGCCTTTACCGGTC
  GACATGGTTCTCATCTCCCTTTGCTTTGGACTCAGCATTGCAACCATGGTGCAGTGCTTT
  GGCCATATCAGCGGTGGCCACATCAACCCTGCAGTGACTGTGGCCATGGTGTGCACCAGG
  AAGATCAGCATCGCCAAGTCTGTCTTCTACATCGCAGCCCAGTGCCTGGGGGCCATCATT
  GGAGCAGGAATCCTCTATCTGGTCACACCTCCCAGTGTGGTGGGAGGCCTGGGAGTCACC
  ATGGTTCATGGAAATCTTACCGCTGGTCATGGTCTCCTGGTTGAGTTGATATCACATTTA
  ATTGGTGTTTACTATCTTGCCAGCTGTGATTCCAACGGACTGATGTCACTGGCTCAATAG
  CTNTAGCATTGGATTTTTCTGTTGCAATTGGACATTTATTTGCAATAATTATACTGGTGC
  CAGCATGAATCCCGCCCGATCCTTTGGACCTGCAGTTATCATGGGGAAATTGGGAAACCA
  TGATTATTTGGGGTTTGGGGCCATCATAGGAACTGTCCTCGCTGGGGGCTTATAAAAAAT
  GGCTTCTGGCCCCAAGGTTGATTCAAACCTCGTTTAAAAGAAGCCTCAGCAAAGCTGCCA
  GCAACAAAAGAACTCNTGGGGGGGGGGAGACAACCGAATCAGTAAAAACGGTGACTGATC
  TAACCGGAGGGGGCTGTGAATGACCCCCCCCGGAAAAGAAAAAAGGGAAAACACTTGGGA
  AGGTTTGTTTTATAGACTAAAAACCCCTGAACAACAAAATTTTAAAACGGCTGAATTTTT
  CCCCTAGGGAAAAATGGTAAAAAAAAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004028 unedited
  ATGGACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATGTTTACCTT
  TTAAAACATCCAAATGTTTATTTTCACAAGCTTAGTAGTACAAGATGCAACATAATTCAA
  GAAAATCAACCACAGTGCTAATACTTTAATATATTATTCCAAATATTAGACGTTACAGTT
  TAAATCTTTTTAACATTAGCTCTAATGTTGGAATAACATTTAAGTTAATAAATAACTGTG
  AATATAACCATATGTTCAGTCTATTCTCCCCAGTTTATCCTAAATGACAATTTTATTGTG
  ATTTGGGTCATAAAAGTGATAAATAATTGTTTATATGTTTTCATAAATTGCATGTTTATT
  TAATTAATCCTGAAGAGAACCTAGAAATACATTCCAATGGGTTACATAAATTAGACGTAT
  TTTTTAGCTCTTCGATTTTTTTTTAAACATGAGTGAGTCACACTGTAACTGCTCTTAAAT
  TTAGTGTTCATTGCACATAACATTTTTTGAAATGTATTTTCACAGGCTATAGGTAGTCAT
  TTGCAAAGATGGCCACAATTTTTGATCCTTGAATTAAATGTCTTTCATTTATTTCAGAGA
  ATTATGAGTTTCAAAAGCTACTGCTCTTATGGNGCAATCTGTAGAGTACTAAACTCACAA
  AATTTGTGGTAACAAAAGAGAGTTTTGTTACATTACACTTTNNCAATAGNTACCACATCC
  TCCTACTAATTTTCATGGTAAAAACACTTTANGGAAAGACAGAANTACTTTTTAGTATAA
  AATGGTTCTGAATTTGCAAANTCTATAGTGCTTATGAGAATTATATTGGGCTTTATATGG
  GTTGATAAGGTGNCTTGTGCCCCCAGACTTTCATCTCAGGTGCCACAAAGGCAATCTCTG
  TGAATGGTCGTACGGGAGGTTGTGTCAAATGCTGCATCNGAGCCTCTAGATCATCTACAT
  TGATTACTGCCTAGTTGAATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004028
• Insert Size: 5000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004028.3, NP_004019.1
• RefSeq Size: 5444 bp
• RefSeq ORF: 906 bp
• Locus ID: 361
• Cytogenetics: 18q11.2
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. Additional isoforms, resulting from the use of alternative in-frame translation initiation codons, have also been described. Recent studies provided evidence for translational readthrough in this gene, and expression of C-terminally extended isoforms via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Jun 2018]'
  Transcript Variant: This variant (2, also known as hMIWC1) is alternatively spliced at the 5' end, which causes translation initiation from an in-frame, downstream start codon, compared to variant 1. Variant 2 encodes two isoforms, resulting from the use of alternative in-frame translation termination codons. The encoded isoform (M23), which results from the use of the upstream UGA stop codon, has a shorter N-terminus compared to isoform M1. This isoform is the same as that represented in NM_001364286.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.