Rad6 (UBE2A) (NM_181777) Human Untagged Clone

CAT#: SC127386

UBE2A (untagged)-Human ubiquitin-conjugating enzyme E2A (UBE2A), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBE2A
• Synonyms: HHR6A; MRXS30; MRXSN; RAD6A; UBC2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_181777, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTCCATCCAAATGTCTATGCAGATGGTAGTATATGTCTGGACATACTTCAGAACCGTTGGAGTCCAA
  CCTATGATGTGTCTTCCATTCTAACATCCATACAGTCTCTGTTGGATGAACCCAATCCCAATAGTCCAGC
  AAACAGCCAGGCTGCTCAGCTGTACCAGGAGAACAAACGGGAATATGAAAAGCGTGTTTCTGCAATAGTA
  GAACAAAGCTGGCGTGATTGTTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_181777 unedited
  TCATAATTTGTATACGACTCACTATAGGCGGCACGCGAATTCGCACCAGGCGGTTCCTCT
  GGGTGCTTCCGCCTCCCCTTCTCCTGCTTCTCCAGCCTCTTCGGCCTCCTCGCCCGCCGC
  GGGAACCCGAGACCCCAGTGTATGCCCCACCCCTGACCCCGCTCGCGACATGTCCACCCC
  GGCTCGGCGGCGCCTCATGCGGGACTTCAAGAGGTTGCAGGAGGATCCTCCAGCCGGAGT
  CAGCGGGGCTCCGTCCGAGAACAACATAATGGTGTGGAACGCGGTCATTTTCGGGCCTGA
  AGGGACCCCGTTTGAGGATGGAACATTTAAACTTACAATAGAATTCACTGAAGAATATCC
  AAATAAACCACCTACAGTTAGATTTGTCTCTAAGATGTTCCATCCAAATGTCTATGCAGA
  TGGTAGTATATGTCTGGACATACTTCAGAACCGTTGGAGTCCAACCTATGATGTGTCTTC
  CATTCTAACATCCATACAGTCTCTGTTGGATGAACCCAATCCCAATAGTCCAGCAAACAG
  CCAGGCTGCTCAGCTGTACCAGGAGAACAAACGGGAATATGAAAAGCGTGTTTCTGCAAT
  AGTAGAACAAAGCTGGCGTGATTGTTGACCCCAGGTACAGTTTATAGAAGCTGGCCATAA
  GATAAAATATATTGATGTGTTTGTCACCTCCCTACTCCTGTCATTACATTTACTGTATTA
  AAAGCGAAATAACTGGTGTGCTGTTTCCATCCTTCTTGCCAAGGTTTACTACCCCTTCTA
  CCCTCTCCTTAAACATCAGAAACACCCTCTATGAAATAAATGTACTGTACCTGGGTTACT
  TNGCAAAATACTATGGCTCAGNTNNTCTGTTGGATTCATTTCCAGNTTCAGGCAGNTATT
  TTTATTATGGACTCTAAGCTTTAGAAGAAATGTATACAGAAGTGCTATGCTNACCTGAGA
  C
  
• Restriction Sites: NotI-NotI
• ACCN: NM_181777
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181777.1, NP_861442.1
• RefSeq Size: 2678 bp
• RefSeq ORF: 234 bp
• Locus ID: 7319
• Cytogenetics: Xq24
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: 'The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]'
  Transcript Variant: This variant (3) lacks several 5' exons but has an alternate 5' segment, as compared to variant 1. It uses a downstream in-frame start codon and encodes isoform 3, which has a shorter N-terminus than that of isoform 1.