RNF6 (NM_183044) Human Untagged Clone

CAT#: SC127416

RNF6 (untagged)-Human ring finger protein (C3H2C3 type) 6 (RNF6), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RNF6
• Synonyms: DKFZp686P0776
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127416 sequence for NM_183044 edited (data generated by NextGen Sequencing)
  ATGAATCAGTCTAGATCGAGATCAGATGGTGGCAGTGAAGAAACCTTACCTCAAGACCAT
  AATCATCATGAAAATGAGAGAAGATGGCAGCAAGAGCGTCTCCACAGAGAAGAGGCCTAT
  TATCAGTTTATTAATGAACTCAATGATGAAGATTATCGGCTTATGAGAGACCATAATCTT
  TTAGGCACCCCTGGAGAAATAACATCAGAAGAACTGCAACAGCGGTTAGATGGCGTCAAG
  GAACAACTAGCATCTCAGCCTGACTTGAGAGATGGAACGAATTACAGAGACTCAGAAGTC
  CCTAGAGAAAGTTCACATGAAGATTCTCTTCTAGAATGGTTGAACACCTTTCGGCGCACA
  GGAAATGCAACTCGAAGTGGACAAAATGGGAACCAAACTTGGAGAGCTGTGAGTCGAACA
  AACCCGAACAATGGAGAGTTTCGGTTTAGTTTGGAAATCCACGTAAATCATGAAAATAGA
  GGATTTGAAATTCATGGAGAAGATTATACAGACATTCCACTTTCAGATAGTAACAGAGAT
  CATACTGCAAATAGGCAACAAAGGTCAACTAGTCCTGTGGCTAGGCGAACAAGAAGCCAA
  ACCTCAGTGAATTTCAATGGTAGTAGTTCCAACATTCCAAGGACTAGGCTTGCTTCAAGG
  GGGCAAAATCCAGCTGAAGGATCTTTCTCAACATTGGGAAGGTTAAGAAATGGAATTGGG
  GGAGCAGCTGGCATTCCTCGAGCTAACGCTTCACGCACTAATTTCAGTAGTCACACAAAC
  CAATCAGGTGGTAGTGAACTCAGGCAAAGGGAGGGGCAACGGTTTGGAGCAGCACATGTT
  TGGGAAAATGGGGCTAGAAGTAATGTTACAGTGAGGAATACAAACCAAAGATTAGAGCCA
  ATAAGATTACGATCTACTTCCAATAGTCGAAGCCGTTCACCAATTCAGAGACAGAGTGGC
  ACTGTTTATCATAATTCCCAAAGGGAAAGTAGACCAGTACAGCAAACCACTAGAAGATCT
  GTTAGGAGGAGAGGTAGAACTCGAGTCTTTTTAGAGCAAGATAGAGAACGAGAACGCAGA
  GGTACTGCATATACCCCATTCTCTAATTCAAGGCTTGTGTCAAGAATAACAGTAGAAGAA
  GGAGAAGAATCCAGCAGATCCTCAACTGCTGTACGACGACATCCAACAATCACACTGGAC
  CTTCAAGTGAGAAGGATCCGTCCTGGAGAAAATAGAGATCGGGATAGTATTGCAAATAGA
  ACTCGATCCAGAGTAGGGCTAGCAGAAAATACAGTCACTATTGAAAGCAATAGTGGGGGC
  TTTCGCCGAACCATTTCTCGTTTAGAGCGGTCAGGTATTCGAACCTATGTTAGTACCATA
  ACAGTTCCTCTTCGTAGGATTTCTGAGAATGAGCTTGTTGAGCCATCATCAGTGGCTCTT
  CGGTCAATTTTAAGGCAGATCATGACTGGGTTTGGAGAACTGAGTTCTCTAATGGAGGCC
  GATTCTGAGTCAGAACTTCAAAGAAATGGCCAGCATTTACCAGACATGCACTCAGAACTG
  AGTAACTTAGGTACAGATAACAACAGGAGCCAGCACAGGGAAGGTTCCTCTCAAGACAGG
  CAGGCCCAAGGAGACAGCACTGAAATGCATGGTGAAAACGAGACCACCCAGCCTCATACT
  CGAAACAGTGACAGTAGGGGTGGCAGGCAGTTGCGAAATCCAAACAATTTAGTTGAAACT
  GGAACACTACCCATTCTTCGCCTTGCTCACTTTTTTTTACTAAATGAAAGTGATGATGAT
  GATCGAATACGTGGTTTAACCAAAGAGCAGATTGACAATCTTTCCACCAGGCACTATGAG
  CATAACAGTATTGATAGTGAACTAGGTAAAATCTGTAGTGTTTGTATTAGTGACTATGTA
  ACTGGAAACAAGCTCAGGCAATTACCTTGCATGCATGAATTTCACATTCATTGTATTGAC
  CGATGGCTCTCAGAGAATTGCACTTGTCCGATCTGTCGGCAGCCTGTTTTAGGGTCTAAC
  ATAGCAAACAATGGGTAA
  
  Clone variation with respect to NM_183044.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_183044 unedited
  GCCGCGAATTCGGCACCAGGGTCAGCTCTCAAAAAAGGCACAAACAATTGAAGGATGGAT
  ACCATGGCATATGTTAAAAGCGTGTTGAAAGGAAAATAAGAAAGCCAGGAATCTCAGGAT
  GAATCAGTCTAGATCGAGATCAGATGGTGGCAGTGAAGAAACCTTACCTCAAGACCATAA
  TCATCATGAAAATGAGAGAAGATGGCAGCAAGAGCGTCTCCACAGAGAAGAGGCCTATTA
  TCAGTTTATTAATGAACTCAATGATGAAGATTATCGGCTTATGAGAGACCATAATCTTTT
  AGGCACCCCTGGAGAAATAACATCAGAAGAACTGCAACAGCGGTTAGATGGCGTCAAGGA
  ACAACTAGCATCTCAGCCTGACTTGAGAGATGGAACGAATTACAGAGACTCAGAAGTCCC
  TAGAGAAAGTTCACATGAAGATTCTCTTCTAGAATGGTTGAACACCTTTCGGCGCACAGG
  AAATGCAACTCGAAGTGGACAAAATGGGAACCAAACTTGGAGAGCTGGGAGTCGACCAAA
  CCCGACAATGGAGAGTTTCGGTTTAGTTTGGAAATCCACGTAATATGAAATAGAGGATTT
  GAAATCATGGAGAAGATATACAGACATTTCACTTTCAATAGTACAGAGATCATACTGCAA
  ATAGGAACAAGGTCAACTATTCCTGGGGCTGGCGACAAAAGCCAACCTCAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_183044 unedited
  ACTATGGACCGCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTTTTTTTACA
  GTCTTTGTTTTAATGTTTATTGGTAGAAAACAGATCTTCAATGCATACTTTGTGTTTATA
  TAAACTCTACATTCTCTTAAAGGTTTTCGTTTTGTTTTCACTGGAGATTTTTAGCCTCCA
  AGTGAACTTAACATATTGCCTATGCATCTGATTCTTTATAGACTTTTAGATTTTAAAGCT
  AAATTTGAGAAACCATGCATACTGTATACCTTATTTAATAATCCAAAGAATTGTTTGCAC
  TTTCAAAAAAGTTACAAAAAGGCTGAACACAAGTTAAATAACCTATATGATGTAAATTTT
  CCATTTCTGAATACTTTTTCAGTATTATATATTGCTTGCTGTCTAATAAGTTAGATTGTC
  AGAGACGCTTCAGTAAATTATCTCTACTTTAAAATTATATCTGAATCCCCTTTCTCTGAG
  ATGAACTTGCCAATATTAAACATTGTGCCATATGCAGTATTAGCCCAAAAGCTTAAATAA
  GAACCAAACTTGTAGACTGAATATTTTAACCTTAAAATTATATACCTATATATACACCTA
  TGGTATGCTGCATATTAAATTTAACATTTCAAGTAACATATATATAGCAAACATTCAGCC
  AAATACTCTTTCATGAAAAGATACTGCCCTTAAAATAAAAAGTTAATGAAAAGCTTATTT
  AGACACAAATGTCTAGATATAAGTACTAAGCCTATGAAACTTGAAGCTAAAGTCCGCTGT
  ACTATTAAGAAAAGAAGATGATTCTTAACCTACTGAATTGTGCAGATACCAAAGTGCTTA
  NCATGACANAATTACCACAATAAAAACCTTTTAGAGGTTATTTGGGTCCTAGCCATATTA
  ACATTTCTGGTATTCCTGGATAATTAANCTTTGGATTCTTAAATTTAATTAAATTCCTCT
  TAACCAGTTCTAAAAAGCCACGAAAATTTTCAAACTTATTAATCTGTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_183044
• Insert Size: 3300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183044.1, NP_898865.1
• RefSeq Size: 3419 bp
• RefSeq ORF: 2058 bp
• Locus ID: 6049
• Cytogenetics: 13q12.13
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene contains a RING-H2 finger motif. Deletions and mutations in this gene were detected in esophageal squamous cell carcinoma (ESCC), suggesting that this protein may be a potential tumor suppressor. Studies of the mouse counterpart suggested a role of this protein in the transcription regulation that controls germinal differentiation. Multiple alternatively spliced transcript variants encoding the same protein are observed. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) differs in the 5' UTR, as compared to variant 1. Variants 1, 3, and 4 all encode the same isoform (1).