Von Hippel Lindau (VHL) (NM_198156) Human Untagged Clone

CAT#: SC127423

VHL (untagged)-Human von Hippel-Lindau tumor suppressor (VHL), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VHL
• Synonyms: HRCA1; pVHL; RCA1; VHL1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_198156, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCCCGGAGGGCGGAGAACTGGGACGAGGCCGAGGTAGGCGCGGAGGAGGCAGGCGTCGAAGAGTACG
  GCCCTGAAGAAGACGGCGGGGAGGAGTCGGGCGCCGAGGAGTCCGGCCCGGAAGAGTCCGGCCCGGAGGA
  ACTGGGCGCCGAGGAGGAGATGGAGGCCGGGCGGCCGCGGCCCGTGCTGCGCTCGGTGAACTCGCGCGAG
  CCCTCCCAGGTCATCTTCTGCAATCGCAGTCCGCGCGTCGTGCTGCCCGTATGGCTCAACTTCGACGGCG
  AGCCGCAGCCCTACCCAACGCTGCCGCCTGGCACGGGCCGCCGCATCCACAGCTACCGAGTGTATACTCT
  GAAAGAGCGATGCCTCCAGGTTGTCCGGAGCCTAGTCAAGCCTGAGAATTACAGGAGACTGGACATCGTC
  AGGTCGCTCTACGAAGATCTGGAAGACCACCCAAATGTGCAGAAAGACCTGGAGCGGCTGACACAGGAGC
  GCATTGCACATCAACGGATGGGAGATTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_198156 unedited
  ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCCGGGTGGTCTGGATCGCG
  GAGGGAATGCCCCGGAGGGCGGAGAACTGGGACGAGGCCGAGGTAGGCGCGGAGGAGGCA
  GGCGTCGAAGAGTACGGCCCTGAAGAAGACGGCGGGGAGGAGTCGGGCGCCGAGGAGTCC
  GGCCCGGAAGAGTCCGGCCCGGAGGAACTGGGCGCCGAGGAGGAGATGGAGGCCGGGCGG
  CCGCGGCCCGTGCTGCGCTCGGTGAACTCGCGCGAGCCCTCCCAGGTCATCTTCTGCAAT
  CGCAGTCCGCGCGTCGTGCTGCCCGTATGGCTCAACTTCGACGGCGAGCCGCAGCCCTAC
  CCAACGCTGCCGCCTGGCACGGGCCGCCGCATCCACAGCTACCGAGGTCACCTTTGGCTC
  TTCAGAGATGCAGGGACACACGATGGGCTTCTGGTTAACCAAACTGAATTATTTGTGCCA
  TCTCTCAATGTTGACGGACAGCCTATTTTTGCCAATATCACACTGCCAGTGTATACTCTG
  AAAGAGCGATGCCTCCAGGTTGTCCGGAGCCTAGTCAAGCCTGAGAATTACAGGAGACTG
  GACATCGTCAGGTCGCTCTACGAAGATCTGGAAGACCACCCAAATGTGCAGAAAGACCTG
  GAGCGGCTGACACAGGAGCGCATTGCACATCAACGGATGGGAGATTGAAGATTTCTGNTG
  AAACTACACTGTTTCATCTCAGCTTTTGATGGTACTGATGAGTCTTGATCTAAATCAGGA
  CTGGCTCCTTTCTTAGTTTCAAGTGTCTCATTCTCAGAGTAAATAGGCACCTTGCTTAAA
  AGAAAGTACTGACTTCCTAGCATTGTGAGTTTAGGGGCAACATACCAATGTAATTTATGC
  CTGCCATANAGAGTATTATCACGAGAAGGTGTGGCTTTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_198156 unedited
  ACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGGCAGGGTCTTGCT
  CTGTCCCCCAGCTGGAGTGCAGTGGCATACTCATGGCTCACTGCCGCCTCCAATTCCTAA
  ACTCCAATGATCTTCTCACCTCAGCCTGCCAAGTAGCTAGGATTACAGATGTGTGCCGCC
  ATGCTAATTTTTAAAAATTTTTTGTATAGATGGGTTCTCACTATGAGCCCAGGCTCATCT
  TGAAGTTCTGGGCTCAAGCAATCCTCTTACCTTGGCTTCCCAAAGTACTGGGATTACAGG
  CGGGAGCCACCATGCCAGCCGTCACTCTCATTTTTGAGCACAGGAATTGTGCCCAAAGGC
  TAGGATAAGGAATTAAAGATGCATGCCAAGAAATACCCACAAACATTAATTATAAAAGGG
  AGACTATACTAATGTTTGTGAAAAGCTTATACTAACATAGAGCAATATTTAAGTTATAAA
  GTTAAATGAAAAAGGGTATAAAACTGTATGAACAGTACAATCTCAACTATGTAAAAACCA
  ACCAAAATCTGCCCTAAAAAGGCTGGAAGGAAATAGCTCAAAATGTTAATGTGATTGTAT
  TTAAGAAGTGTATCTAATAGGGATCTTTTTTCTTCCTTTTTAGTTTTCTGTCTTTTCCAA
  ATGTTTCTCTACTGGGGAGATTTTTTTAAAAAGCTATTAGGGAAAGAGTACAACAAAAGT
  GGAAAAAAAAACTATTAGTTACAAAAAACTGAGTCTGATAAAACCATGAAATTGAGTTAT
  GGAACCTAAGTCTAACCTTTAGGATTTGTACTTTAGCAAACTATGAAACAGCCCAGCTCC
  TCATTGATCCATGGGAGGACCCAAGCTGGAGGTGAAAGCCCCCTGGCCTGCCTGTAAAAA
  TGGCGATAGCCGGCCCGGGGCCCAACCCTGTATCCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_198156
• Insert Size: 2990 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198156.1, NP_937799.1
• RefSeq Size: 2845 bp
• RefSeq ORF: 519 bp
• Locus ID: 7428
• Cytogenetics: 3p25.3
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis
• Gene Summary: 'Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks an in-frame coding exon compared to variant 1. The resulting isoform (2) lacks an internal region, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.