Tropomyosin 2 (TPM2) (NM_003289) Human Untagged Clone

CAT#: SC127609

TPM2 (untagged)-Human tropomyosin 2 (beta) (TPM2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TPM2
• Synonyms: AMCD1; DA1; DA2B; DA2B4; HEL-S-273; NEM4; TMSB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127609 sequence for NM_003289 edited (data generated by NextGen Sequencing)
  ATGGACGCCATCAAGAAGAAGATGCAGATGCTGAAGCTGGACAAGGAGAACGCCATCGAC
  CGCGCCGAGCAGGCCGAAGCCGACAAGAAGCAAGCTGAGGACCGCTGCAAGCAGCTGGAG
  GAGGAGCAGCAGGCCCTCCAGAAGAAGCTGAAGGGGACAGAGGATGAGGTGGAAAAGTAT
  TCTGAATCCGTGAAGGAGGCCCAGGAGAAACTGGAGCAGGCCGAGAAGAAGGCCACTGAT
  GCTGAGGCAGATGTGGCCTCCCTGAACCGCCGCATTCAGCTGGTTGAGGAGGAGCTGGAC
  CGGGCCCAGGAGCGCCTGGCTACAGCCCTGCAGAAGCTGGAGGAGGCCGAGAAGGCGGCT
  GATGAGAGCGAGAGAGGAATGAAGGTCATCGAAAACCGGGCCATGAAGGATGAGGAGAAG
  ATGGAACTGCAGGAGATGCAGCTGAAGGAGGCCAAGCACATCGCTGAGGATTCAGACCGC
  AAATATGAAGAGGTGGCCAGGAAGCTGGTGATCCTGGAAGGAGAGCTGGAGCGCTCGGAG
  GAGAGGGCTGAGGTGGCCGAGAGTAAATGTGGGGACCTAGAGGAGGAGCTGAAAATTGTT
  ACCAACAACTTGAAATCCCTGGAGGCCCAGGCGGACAAGTATTCCACCAAAGAAGATAAA
  TATGAAGAGGAGATCAAACTGTTGGAGGAGAAGCTGAAGGAGGCTGAGACCCGAGCAGAG
  TTTGCCGAGAGGTCTGTGGCAAAGTTGGAGAAAACCATCGATGACCTAGAAGATGAAGTC
  TATGCCCAGAAGATGAAGTACAAGGCCATTAGCGAGGAACTGGACAACGCACTCAATGAC
  ATCACCTCCCTCTGA
  
  Clone variation with respect to NM_003289.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003289 unedited
  GTCAAATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGTCCCTCCCCGC
  CTTTTAGGCGCCCGCGTGGCCGGGACGTCCCAGTCCCGCTCCGTCCTCCTCGCCTGCCAC
  CGGTGCACCCAGTCCGCTCACCCAGCCCAGTCCGTCCGGTCCTCACCGCCTGCCGGCCGG
  CCCACCCCCCACCGCAGCCATGGACGCCATCAAGAAGAAGATGCAGATGCTGAAGCTGGA
  CAAGGAGAACGCCATCGACCGCGCCGAGCAGGCCGAAGCCGACAAGAAGCAAGCTGAGGA
  CCGCTGCAAGCAGCTGGAGGAGGAGCAGCAGGCCCTCCAGAAGAAGCTGAAGGGGACAGA
  GGATGAGGTGGAAAAGTATTCTGAATCCGTGAAGGAGGCCCAGGAGAAACTGGAGCAGGC
  CGAGAAGAAGGCCACTGATGCTGAGGCAGATGTGGCCTCCCTGAACCGCCGCATTCAGCT
  GGTTGAGGAGGAGCTGGACCGGGCCCAGGAGCGCCTGGCTACAGCCCTGCAGAAGCTGGA
  GGAGGCCGAGAAGGCGGCTGATGAGAGCGAGAGAGGAATGAAGGTCATCGAAAACCGGGC
  CATGAAGGATGAGGAGAAGATGGAACTGCAGGAGATGCAGCTGAAGGAGGCCAAGCACAT
  CGCTGAGGATTCAGACCGCANATATGAAGAGGTGGCCAGNAAGCTGGTGATCCTGGAAGG
  AGAGCTGNACCGCTCCGNAGAAAAGGNCTGANNGTGCCGANAAGTAATGTGGNGACCTAG
  NNAGAGAGCTGTAAATTGTTACCAACACTTGAAATCCCTGGAGGCCCAGGCGGACAAGTT
  TCCACCAAGAAGAAAATATGAGGAGGAGATCAACTGTTGNAGGAAAGCTGAAAGAGCTGA
  GACCGANCAAANTGCCCGAAAGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003289 unedited
  CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTCCAGTTCAGAA
  TTTATTAAGCAGCAAAGGAGGGTGGAAGGGGATAGGTAAAGGATGAAGCCAGTGCCAAAG
  TGGGTGGTGGGCATGATGGGGGCCTTCCCTAGGCTGCTCCCAGCCTGGCTGTGCAATGTT
  GGCAATTTCTGCTCCTCCTGCCTGCTCCCCTCCCCATAGAGAGAATGGAAAGGAGAGGAG
  AGAAGAGAGCTGAGGTGGCCACGCTGGCGTGGGGCTCAAAGGGAGGTGATGTCATTGAGT
  GCGTTGTCCAGTTCCTCGCTAATGGCCTTGTACTTCATCTTCTGGGCATAGACTTCATCT
  TCTAGGTCATCGATGGTTTTCTCCAACTTTGCCACAGACCTCTCGGCAAACTCTGCTCGG
  GTCTCAGCCTCCTTCAGCTTCTCCTCCAACAGTTTGATCTCCTCTTCATATTTATCTTCT
  TTGGTGGAATACTTGTCCGCCTGGGCCTCCAGGGATTTCAAGTTGTTGGTAACAATTTTC
  AGCTCCTCCTCTAGGTCCCCACATTTACTCTCGGCCACCTTAACCCTCTTCTCCGAGCGC
  TCCAGTTCTCCTTTCAAGATCACCCAGCTTCCTGGCCACCTCTTCTTATTGCGGCCCTGA
  TTCCCAACGATGTGCTTGGCCCCCTTCACCTGCATCTCCTGCAGGTCCTTTTTCTCCTAT
  CCTTTAGGGCCCGGTTTTCGATGACCTTTTTTCCTCTTCTGCTTCTTATAACCGGCTTTT
  TTGCCTTCTCCAATTTTTGATGGTTGTAACCAGGCCCTTTCTGGCCCGGTCCACTTCTCT
  CTAACCAATTGATGCGGGGGTTCTAGGAAGCCCTTCTTCTCTTAAATCAGGCCCTTTTTT
  CGCCTCCTCCATTCTTCCTGGGCCCTTACGGATTTAAAACTTCTTCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003289
• Insert Size: 1360 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003289.3, NP_003280.2
• RefSeq Size: 1327 bp
• RefSeq ORF: 855 bp
• Locus ID: 7169
• Cytogenetics: 9p13.3
• Domains: Tropomyosin
• Protein Pathways: Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)
• Gene Summary: 'This gene encodes beta-tropomyosin, a member of the actin filament binding protein family, and mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]'
  Transcript Variant: This variant (Tpm2.2, also known as variant 1) represents the longer transcript and encodes isoform Tpm2.2st (also known as isoform 1).