UBE2D3 (NM_181892) Human Untagged Clone

CAT#: SC127645

UBE2D3 (untagged)-Human ubiquitin-conjugating enzyme E2D 3 (UBE2D3), transcript variant 8

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBE2D3
• Synonyms: E2(17)KB3; UBC4/5; UBCH5C
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_181892, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGCTGAAACGGATTAATAAGGAACTTAGTGATTTGGCCCGTGACCCTCCAGCACAATGTTCTGCAG
  GTCCAGTTGGGGATGATATGTTTCATTGGCAAGCCACAATTATGGGACCTAATGACAGCCCATATCAAGG
  CGGTGTATTCTTTTTGACAATTCATTTTCCTACAGACTACCCCTTCAAACCACCTAAGGTTGCATTTACA
  ACAAGAATTTATCATCCAAATATTAACAGTAATGGCAGCATTTGTCTCGATATTCTAAGATCACAGTGGT
  CGCCTGCTTTAACAATTTCTAAAGTTCTTTTATCCATTTGTTCACTGCTATGTGATCCAAACCCAGATGA
  CCCCCTAGTGCCAGAGATTGCACGGATCTATAAAACAGACAGAGATAAGTACAATAGGTTAGCAAGAGAG
  TGGACAGAGAAATACGCTATGTTGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_181892 unedited
  TGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGTTCCGAGAAGGAGAAGG
  AAAGGGCTTGACACGTATTCACTCGGCCCCGGACGTGGGAAGCAAGCCGTCTGGCTTCGG
  CCTCACATCGGTCCTGTGCTCGGGACGGCGGCGTTGGCGGACTGATCCGCGGCGGTGAAG
  AGGCGCCTGTGTCTGGCAGAGCTGGTGTGAGACGAGACAATCCTGCCCCGCCGCCGGGAT
  AATCAAGAGTTTTGGCCGGACCTTTGAGCATACACCGAGAGAGTGAGGAGCCAGACGACA
  AGCACACACTATGGCGCTGAAACGGATTAATAAGGAACTTAGTGATTTGGCCCGTGACCC
  TCCAGCACAATGTTCTGCAGGTCCAGTTGGGGATGATATGTTTCATTGGCAAGCCACAAT
  TATGGGACCTAATGACAGCCCATATCAAGGCGGTGTATTCTTTTTGACAATTCATTTTCC
  TACAGACTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAA
  TATTAACAGTAATGGCAGCATTTGTCTCGATATTCTAAGATCACAGTGGTCGCCTGCTTT
  AACAATTTCTAAAGTTCTTTTATCCATTTGTTCACTGCTATGTGATCCAAACCCAGATGA
  CCCCCTAGTGCCAGAGATTGCACGGATCTATANAACAGACAGAGATAAGTACAACAGAAT
  ATCTCGGGAATGGACTCAGAAGTATGCCATGTGATGCTACCTTTAAGTCAGAATAACCTG
  CATTATAGCTGGAATAAAACTTTAATACTGGTCCTTTTTTGATTTTCTATCCGGCTGCTT
  CCTATCAGACTCATCTTTTTATTTTATTTTTGTTACCTCCTCCTTCTTCCATGCTCATTG
  AAGACTTAGTCTTCACTTGACATACTGTTTAAACGTAAGAGTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_181892 unedited
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTTTATGCAACTTT
  ATTGAAGAAAAATAAATCAATTACTAGCAGAGCTATTAGTTGATCACTCATCCATTGACA
  ACTTGCATCATTTATTCAGTGCTATATTAAACAGTGTATTGGAAGATAGATTAACTAATA
  GCTCCAAGCCTCCTAACAATTTAAATGAAAATTACAAAATGTTTGAGACCCTATTTTGGA
  ATACAAAGGGTGTTTGACTTCCAATTTCCATTCTCTGTAGAACAAGAACAGGTCATTCCT
  TTATTGACATGCATAAAATACATCACATTTTCTGTTCTGCTGATGCTATAAATTCAATAC
  CAATTCTCCAGCCACATCAGTTATGAGCATAAAGCATATCATGTAACCTCAAATCTCTAA
  CAGTGGAAGGCTTAAACAAGAATGGATGCTGCTAGAATAATGCTGTATCCTTTGATGTGA
  CAACTGAGCATCCATCTCCCTCCCCCTCAGATGATTTCTTCAGCATGTTAGAGCAGTGAG
  GAATGGTTTTAGTCTCATAACCAAGTTAGAGTGAAGACATTGGCCACATAATACACATGC
  TCCATTTTTTTTTTTAAAAATTCTGAGTCTTGGGCAACTGTTCTCTTGTAACTACTTTAC
  AGTTTCTAAAAGCAGGTATTGTCCAAAGCTGGAAGAACTTAAGTCTTCTCAAATGACCAT
  GTGAATGAATGGAAGGAGGTAACCAAAAATAAAATTAAAAAGATGAGGCCTGATAGGGGA
  GCCACCCGTATAAAAAATCAAAAAGAACCAGGTTTTAAGTTTATTCCACCTTATGGCCGG
  TTTTCTGACTTTAGGTAGCTACATGGCTACTTTGAACCTTTCCGGAATTTCGTGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_181892
• Insert Size: 1670 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181892.1, NP_871621.1
• RefSeq Size: 4216 bp
• RefSeq ORF: 447 bp
• Locus ID: 7323
• Cytogenetics: 4q24
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: 'The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. [provided by RefSeq, Jan 2017]'
  Transcript Variant: This variant (8) differs in the 5' UTR and has an additional exon in the 3' region, as compared to variant 1. It encodes isoform 2, which has a distinct and one aa longer C-terminus, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.