TJP1 (NM_003257) Human Untagged Clone

CAT#: SC127835

TJP1 (untagged)-Human tight junction protein 1 (zona occludens 1) (TJP1), transcript variant 1


  "NM_003257" in other vectors (5)

Reconstitution Protocol

SC127835 is the updated version of SC120780.

USD 2,820.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol TJP1
Synonyms ZO-1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_003257, the custom clone sequence may differ by one or more nucleotides


ATGTCCGCCAGAGCTGCGGCCGCCAAGAGCACAGCAATGGAGGAAACAGCTATATGGGAACAACATACAG
TGACGCTTCACAGGGCTCCTGGATTTGGATTTGGAATTGCAATATCTGGTGGACGAGATAATCCTCATTT
TCAGAGTGGGGAAACGTCAATAGTGATTTCAGATGTGCTGAAAGGAGGACCAGCTGAAGGACAGCTACAG
GAAAATGACCGAGTTGCAATGGTTAACGGAGTTTCAATGGATAATGTTGAACATGCTTTTGCTGTTCAGC
AACTAAGGAAAAGTGGGAAAAATGCAAAAATTACAATTAGAAGGAAGAAGAAAGTTCAAATACCAGTAAG
TCGTCCTGATCCTGAACCAGTATCTGATAATGAAGAAGATAGTTATGATGAGGAAATACATGATCCAAGA
AGTGGCCGGAGTGGTGTGGTTAACAGAAGGAGTGAGAAGATTTGGCCGAGGGATAGAAGTGCAAGTAGAG
AGAGGAGCTTGTCCCCGCGGTCAGACAGGCGGTCAGTGGCTTCCAGCCAGCCTGCTAAACCTACTAAAGT
CACACTGGTGAAATCCCGGAAAAATGAAGAATATGGTCTTCGATTGGCAAGCCATATATTTGTTAAGGAA
ATTTCACAAGATAGTTTGGCAGCAAGAGATGGCAATATTCAAGAAGGTGATGTTGTATTGAAGATAAATG
GTACTGTGACAGAAAATATGTCATTGACAGATGCAAAGACATTGATAGAAAGGTCTAAAGGCAAATTAAA
AATGGTAGTTCAAAGAGATGAACGGGCTACGCTATTGAATGTCCCTGATCTTTCTGACAGCATCCACTCT
GCTAATGCCTCTGAGAGAGACGACATTTCAGAAATTCAGTCACTGGCATCAGATCATTCTGGTCGATCAC
ACGATAGGCCTCCCCGCCGCAGCCGGTCACGATCTCCTGACCAGCGGTCAGAGCCTTCTGATCATTCCAG
GCACTCGCCGCAGCAGCCAAGCAATGGCAGTCTCCGGAGTAGAGATGAAGAGAGAATTTCTAAACCTGGG
GCTGTCTCAACTCCTGTAAAGCATGCTGATGATCACACACCTAAAACAGTGGAAGAAGTTACAGTTGAAA
GAAATGAGAAACAAACACCTTCTCTTCCAGAACCAAAGCCTGTGTATGCCCAAGTTGGGCAACCAGATGT
GGATTTACCTGTCAGTCCATCTGATGGTGTCCTACCTAATTCAACTCATGAAGATGGGATTCTTCGGCCC
AGCATGAAATTGGTAAAATTCAGAAAAGGAGATAGTGTGGGTTTGCGGCTGGCTGGTGGAAATGATGTTG
GAATATTTGTAGCTGGCGTTCTAGAAGATAGCCCTGCAGCCAAGGAAGGCTTAGAGGAAGGTGATCAAAT
TCTCAGGGTAAACAACGTAGATTTTACAAATATCATAAGAGAAGAAGCCGTCCTTTTCCTGCTTGACCTC
CCTAAAGGAGAAGAAGTGACCATATTGGCTCAGAAGAAGAAGGATGTTTATCGTCGCATTGTAGAATCAG
ATGTAGGAGATTCTTTCTATATTAGAACCCATTTTGAATATGAAAAGGAATCTCCCTATGGACTTAGTTT
TAACAAAGGAGAGGTGTTCCGTGTTGTGGATACCTTGTACAATGGAAAACTGGGCTCTTGGCTTGCTATT
CGAATTGGTAAAAATCATAAGGAGGTAGAACGAGGCATCATCCCTAATAAGAACAGAGCTGAGCAGCTAG
CCAGTGTACAGTATACACTTCCAAAAACAGCAGGCGGAGACCGTGCTGACTTCTGGAGATTCAGAGGTCT
TCGCAGCTCCAAGAGAAATCTTCGAAAAAGCAGAGAGGATTTGTCCGCTCAGCCTGTTCAAACAAAGTTT
CCAGCTTATGAAAGAGTGGTTCTTCGAGAAGCTGGATTTCTGAGGCCTGTAACCATTTTTGGACCAATAG
CTGATGTTGCCAGAGAAAAGCTGGCAAGAGAAGAACCAGATATTTATCAAATTGCAAAGAGTGAACCACG
AGACGCTGGAACTGACCAACGTAGCTCTGGCATTATTCGCCTGCATACAATAAAGCAAATCATAGATCAA
GACAAACATGCTTTATTAGATGTAACACCAAATGCAGTTGATCGTCTTAACTATGCCCAGTGGTATCCAA
TTGTTGTATTTCTTAACCCTGATTCTAAGCAAGGAGTAAAAACAATGAGAATGAGGTTATGTCCAGAATC
TCGGAAAAGTGCCAGGAAGTTATACGAGCGATCTCATAAACTTCGTAAAAATAATCACCATCTTTTTACA
ACTACAATTAACTTAAATTCAATGAATGATGGTTGGTATGGTGCGCTGAAAGAAGCAATTCAACAACAGC
AAAACCAGCTGGTATGGGTTTCCGAGGGAAAGGCGGATGGTGCTACAAGTGATGACCTTGATTTGCATGA
TGATCGTCTGTCCTACCTGTCAGCTCCAGGTAGTGAATACTCAATGTATAGCACGGACAGTAGACACACT
TCTGACTATGAAGACACAGACACAGAAGGCGGGGCCTACACTGATCAAGAACTAGATGAAACTCTTAATG
ATGAGGTTGGGACTCCACCGGAGTCTGCCATTACACGGTCCTCTGAGCCTGTAAGAGAGGACTCCTCTGG
AATGCATCATGAAAACCAAACATATCCTCCTTACTCACCACAAGCGCAGCCACAACCAATTCATAGAATA
GACTCCCCTGGATTTAAGCCAGCCTCTCAACAGAAAGCAGAAGCTTCATCTCCAGTCCCTTACCTTTCGC
CTGAAACAAACCCAGCATCATCAACCTCTGCTGTTAATCATAATGTAAATTTAACTAATGTCAGACTGGA
GGAGCCCACCCCAGCTCCTTCCACCTCTTACTCACCACAAGCTGATTCTTTAAGAACACCAAGTACTGAG
GCAGCTCACATAATGCTAAGAGATCAAGAACCATCATTGTCGTCGCATGTAGATCCAACAAAGGTGTATA
GAAAGGATCCATATCCCGAGGAAATGATGAGGCAGAACCATGTTTTGAAACAGCCAGCCGTTAGTCACCC
AGGGCACAGGCCAGACAAAGAGCCTAATCTGACCTATGAACCCCAACTCCCATACGTAGAGAAACAAGCC
AGCAGAGACCTCGAGCAGCCCACATACAGATACGAGTCCTCAAGCTATACGGACCAGTTTTCTCGAAACT
ATGAACATCGTCTGCGATACGAAGATCGCGTCCCCATGTATGAAGAACAGTGGTCATATTATGATGACAA
ACAGCCCTACCCATCTCGGCCACCTTTTGATAATCAGCACTCTCAAGACCTTGACTCCAGACAGCATCCC
GAAGAGTCCTCAGAACGAGGGTACTTTCCACGTTTTGAAGAGCCAGCCCCTCTGTCTTACGACAGCAGAC
CACGTTACGAACAGGCACCTAGAGCATCCGCCCTGCGGCACGAAGAGCAGCCAGCTCCTGGGTATGACAC
ACATGGTAGACTCAGACCGGAAGCCCAGCCCCACCCTTCAGCAGGGCCCAAGCCTGCAGAGTCCAAGCAG
TATTTTGAGCAATATTCACGCAGTTACGAGCAAGTACCACCCCAAGGATTTACCTCTAGAGCAGGTCATT
TTGAGCCTCTCCATGGTGCTGCAGCTGTCCCTCCGCTGATACCTTCATCTCAGCATAAGCCAGAAGCTCT
GCCTTCAAACACCAAACCACTGCCTCCACCCCCAACTCAAACCGAAGAAGAGGAAGATCCAGCAATGAAG
CCACAGTCTGTACTCACCAGAGTTAAGATGTTTGAAAACAAAAGATCTGCATCCTTAGAGACCAAGAAGG
ATGTAAATGACACTGGCAGTTTTAAGCCTCCAGAAGTAGCATCTAAACCTTCAGGTGCTCCCATCATTGG
TCCCAAACCCACTTCTCAGAATCAATTCAGTGAACATGACAAAACTCTGTACAGGATCCCAGAACCTCAA
AAACCTCAACTGAAGCCACCTGAAGATATTGTTCGGTCCAATCATTATGACCCTGAAGAAGATGAAGAAT
ATTATCGAAAACAGCTGTCATACTTTGACCGAAGAAGTTTTGAGAATAAGCCTCCTGCACACATTGCCGC
CAGCCATCTCTCCGAGCCTGCAAAGCCAGCGCATTCTCAGAATCAATCAAATTTTTCTAGTTATTCTTCA
AAGGGAAAGCCTCCTGAAGCTGATGGTGTGGATAGATCATTTGGCGAGAAACGCTATGAACCCATCCAGG
CCACTCCCCCTCCTCCTCCATTGCCCTCGCAGTATGCCCAGCCATCTCAGCCTGTCACCAGCGCGTCTCT
CCACATACATTCTAAGGGAGCACATGGTGAAGGTAATTCAGTGTCATTGGATTTTCAGAATTCCTTAGTG
TCCAAACCAGACCCACCTCCATCTCAGAATAAGCCAGCAACTTTCAGACCACCAAACCGAGAAGATACTG
CTCAGGCAGCTTTCTATCCCCAGAAAAGTTTTCCAGATAAAGCCCCAGTTAATGGAACTGAACAGACTCA
GAAAACAGTCACTCCAGCATACAATCGATTCACACCAAAACCATATACAAGTTCTGCCCGACCATTTGAA
CGCAAGTTTGAAAGTCCTAAATTCAATCACAATCTTCTGCCAAGTGAAACTGCACATAAACCTGACTTGT
CTTCAAAAACTCCCACTTCTCCAAAAACTCTTGTGAAATCGCACAGTTTGGCACAGCCTCCTGAGTTTGA
CAGTGGAGTTGAAACTTTCTCTATCCATGCAGAGAAGCCTAAATATCAAATAAATAATATCAGCACAGTG
CCTAAAGCTATTCCTGTGAGTCCTTCAGCTGTGGAAGAGGATGAAGATGAAGATGGTCATACTGTGGTGG
CCACAGCCCGAGGCATATTTAACAGCAATGGGGGCGTGCTGAGTTCCATAGAAACTGGTGTTAGTATAAT
TATCCCTCAAGGAGCCATTCCCGAAGGAGTTGAGCAGGAAATCTATTTCAAGGTCTGCCGGGACAACAGC
ATCCTTCCACCTTTAGATAAAGAGAAAGGTGAAACACTGCTGAGTCCTTTGGTGATGTGTGGTCCCCATG
GCCTCAAGTTCCTGAAGCCTGTGGAGCTGCGCTTACCACACTGTGATCCTAAAACCTGGCAAAACAAGTG
TCTTCCCGGAGATCCAAATTATCTCGTTGGAGCAAACTGTGTTTCTGTCCTTATTGACCACTTTTAA


>OriGene 5' read for NM_003257 unedited
GGCTTTTGTATACGACTCCTATAGGCGGCCGCGAATTCGGCACGAGGGNANAAACTCGGC
TCCAGTGAGGGTTCGGGGGCTGGAAGCCGGCTCTCAGCGGGTCGGGGCTTGGGGTGCCAC
CTCCTGCTGGCCGGGAGCTGCTGTCTTTGGAGGAGTGGTTGGTCCCCGGCGAAACCCTGT
AGTTTCGATCTGATGTCACTCCCTGCGGTATGCGCACGCCAGCGATAAGGCTTTGAGACT
GCAAAACACTCCACTCAGCCTGTGAGGCGTAGTAGAGCACAGCAATGGAGGAAACAGCTA
TATGGGAACAACATACAGTGACGCTTCACAGGGCTCCTGGATTTGGATTTGGAATTGCAA
TATCTGGTGGACGAGATAATCCTCATTTTCAGAGTGGGGAAACGTCAATAGTGATTTCAG
ATGTGCTGAAAGGAGGACCAGCTGAAGGACAGCTACAGGAAAATGACCGAGTTGCAATGG
TTAACGGAGTTTCAATGGATAATGTTGAACATGCTTTTGCTGTTCAGCAACTAAGGAAAA
GTGGGAAAAATGCAAAAATTACAATTAGAAGGAAGAAGAAAGTTCANATACCAGTAAGTC
GTCCTGATCCTGAACCAGTATCTGATAATGAAGAAGATAGTTATGATGAGGAAATACATG
ATCCAAGAAGTGGCCGGAGTGGTGTGGTTAACAGAAGGAGTGAGAAGATTTGGCCGAGGA
TAGAAGTGCAAGTAGAGAGAGGAGCTTGTCCCCGCGGTCAGACAGGCGGTCAGTGGCTTC
CAGCCAGCCTGCTAAACCCTACTAAGTCACACTGGTGAAATCCCGNANAAATGAGATATG
GTCTTCGATTGGCAGCCATATATTGNTAAGGAAATTCACAAAAAGTTTGGGCAGCAGAGA
TGGCATATTCAGAGGGGATGTGTATTGAGAATATGTACTGGACGGAAAATGTCATGN
>OriGene 3' read for NM_003257 unedited
TTTAACANNTTTGACTTGNNACCGCGGCCGCTTACNANNGATCGGTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTATTTTAAAAATTTTATTTTGGGAGATTAGAATTTGAGATTTTTAAT
AACGGCAAAAGAAATCAGTCACACCTAATGATTAACAGAATGTAGTGGTGTATTATCTAA
ACAGAAATCGTGCTGATGTGCCATAATAAATTGTCTATTAGTAAAAAAAACACTTTAGGG
CACAGCATTGTATCACAAATTACAGTAGGGATACTTTGCAAGAATTTAATCAAACTAGAG
AATTCTGAGTAACTGTATCTTTTAAATGCAGCACTTAAAAATGTAACAACTCTGTGCATC
CTTTTTCTTAAAAAAAATGACCTTGCATGTGTCATAGAAACGCTGCTTTATTGCTGCAGA
GGTCAAAGTTCAAGGCTCAAGAGGTACAGGAGAGAATACAAAGGTAGCCTTTAGAAACGT
GGTCTTGTTTATGTATAAAAAAGGTAAAGTTTATAAAAGTTAATTTACAAACCAAGAACA
AAAGTGGTATGCACGCATTATGTACAAGCATCCCTTAAAACATCAAATTTTTCAAATGCA
TAGCCCCGAAGAACCGAAAACCCCCCCCGGCCCCTTTGTCAAAAAAAAAAAAAAAGGGAA
AGGAAAGGAAAGGAAAAATTTTCCCCAAATCCACCCACTATTTTTTTCTGGGGGGATAAC
CATTTTTTGAAACCCCCCAAGGGCCCAAGGTCCTCCAGTTCTTGGCATGGACCCGTTTTT
CAATAAATGCCCCAAAACCCTTTAAACCTTTGTTACCCAGGGGGGAATGGGCCCAAAAAT
TTAAAAAACTGGGGGGTAGAGGCTTTCCCGCCGGGTAAAATTTTCTCGGGGGAAAAAGGG
AGAAATA
Restriction Sites NotI-NotI     
ACCN NM_003257
Insert Size 5000 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003257.1, NP_003248.1
RefSeq Size 7171 bp
RefSeq ORF 5211 bp
Locus ID 7082
Cytogenetics 15q13.1
Domains ZU5, PDZ, Guanylate_kin, GuKc
Protein Families Druggable Genome
Protein Pathways Adherens junction, Epithelial cell signaling in Helicobacter pylori infection, Gap junction, Tight junction, Vibrio cholerae infection
Gene Summary 'This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]'
Transcript Variant: This variant (1, also known as ZO-1alpha+) encodes isoform a, which is found in most epithelial cell junctions.

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