PPP1A (PPP1CA) (NM_001008709) Human Untagged Clone

CAT#: SC127981

PPP1CA (untagged)-Human protein phosphatase 1, catalytic subunit, alpha isozyme (PPP1CA), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP1CA
• Synonyms: PP-1A; PP1A; PP1alpha; PPP1A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001008709, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCCGACAGCGAGAAGCTCAACCTGGACTCGATCATCGGGCGCCTGCTGGAAGGGTCCAGGGTCCTGA
  CACCCCATTGCGCCCCAGTGCAGGGCTCGCGGCCTGGCAAGAATGTACAGCTGACAGAGAACGAGATCCG
  CGGTCTGTGCCTGAAATCCCGGGAGATTTTTCTGAGCCAGCCCATTCTTCTGGAGCTGGAGGCACCCCTC
  AAGATCTGCGGTGACATACACGGCCAGTACTACGACCTTCTGCGACTATTTGAGTATGGCGGTTTCCCTC
  CCGAGAGCAACTACCTCTTTCTGGGGGACTATGTGGACAGGGGCAAGCAGTCCTTGGAGACCATCTGCCT
  GCTGCTGGCCTATAAGATCAAGTACCCCGAGAACTTCTTCCTGCTCCGTGGGAACCACGAGTGTGCCAGC
  ATCAACCGCATCTATGGTTTCTACGATGAGTGCAAGAGACGCTACAACATCAAACTGTGGAAAACCTTCA
  CTGACTGCTTCAACTGCCTGCCCATCGCGGCCATAGTGGACGAAAAGATCTTCTGCTGCCACGGAGGCCT
  GTCCCCGGACCTGCAGTCTATGGAGCAGATTCGGCGGATCATGCGGCCCACAGATGTGCCTGACCAGGGC
  CTGCTGTGTGACCTGCTGTGGTCTGACCCTGACAAGGACGTGCAGGGCTGGGGCGAGAACGACCGTGGCG
  TCTCTTTTACCTTTGGAGCCGAGGTGGTGGCCAAGTTCCTCCACAAGCACGACTTGGACCTCATCTGCCG
  AGCACACCAGGTGGTAGAAGACGGCTACGAGTTCTTTGCCAAGCGGCAGCTGGTGACACTTTTCTCAGCT
  CCCAACTACTGTGGCGAGTTTGACAATGCTGGCGCCATGATGAGTGTGGACGAGACCCTCATGTGCTCTT
  TCCAGATCCTCAAGCCCGCCGACAAGAACAAGGGGAAGTACGGGCAGTTCAGTGGCCTGAACCCTGGAGG
  CCGACCCATCACCCCACCCCGCAATTCCGCCAAAGCCAAGAAATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001008709 unedited
  AACCACGGGGACNNCCACCCCACCACGCCGGTTCAGATTTGTATACGACTCATATAGGCG
  GCCGCGAAATCGGCACCAGGTCCGACAGCGAGNAACTCAACCTGGACTCGATCATCGGGC
  GCCTGCTGGAAGGGTCCAGGGTCCTGACACCCCATTGCGCCCCAGTGCAGGGCTCGCGGC
  CTGGCAAGAATGTACAGCTGACAGAGAACGAGATCCGCGGTCTGTGCCTGAAATCCCGGG
  AGATTTTTCTGAGCCAGCCCATTCTTCTGGAGCTGGAGGCACCCCTCAAGATCTGCGGTG
  ACATACACGGCCAGTACTACGACCTTCTGCGACTATTTGAGTATGGCGGTTTCCCTCCCG
  AGAGCAACTACCTCTTTCTGGGGGACTATGTGGACAGGGGCAAGCAGTCCTTGGAGACCA
  TCTGCCTGCTGCTGGCCTATAAGATCAAGTACCCCGAGAACTTCTTCCTGCTCCGTGGGA
  ACCACGAGTGTGCCAGCATCAACCGCATCTATGGTTTCTACGATGAGTGCAAGAGACGCT
  ACAACATCAAACTGTGGAAAACCTTCACTGACTGCTTCAACTGCCTGCCCATCGCGGCCA
  TAGTGGACGAAAAGATCTTCTGCTGCCACGGAGGCCTGTCCCCGGACCTGCAGTCTATGG
  AGCAGATTCGGCGGATCATGCGGCCCACAGATGTGCCTGACCAGNGCCTGCTGTGTGACC
  TGCTGTGGTCTGACCCCTGACAAGACGTGCANGGCTGGGGCGAGAACGACCGTGCGTCTC
  TTTTACCTTTGGAGCCGAGTGGTGGCCAAGTCCTCCACAGCACGACTTGGACCTCATCTG
  CCGACACACCAGTGGTAGAAGACGCCTATGAGGTCTTTGCCAAGCGCAGCTGGTGACACT
  TTCTCAGCTCCAACTACTGTGNCGAGTTTGACAATGCTGGCGCCATGATGATGTGGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001008709 unedited
  GAGCACTGGNGGNAGGGGTCACAGGGCATGCCACCCGGGTACTGTTCAGGAAACAGCTAT
  GACCGCGGCCGCAATCTAGAGCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCGCTTTGCCCTTTATAA
  AAAAAACCAAAGGGGTGGCCCCAAAACCCGGGGGCCCCCCCGGAGGCCAAACCGGGGGGG
  AAACCCAAAACCGGGCCGGCCGTTGCCCGGACCTGCACCCCCGGCCCCAGGATCCTGGTA
  AAAGCCCCCGGAGGGGCAGGCAAAAAACACCCCGGGGGGACGGGCCCCCCCTTTTGTTTC
  TTTAAAAAAAAAAAAAAAAAATCCCCCGGGGCCCCTTGTCCCCCGGAACAGGGGGCCCGG
  AGGGGCCGGGGGACCCCCCCCCCAACAGGGGAGCAGGATTTTGGAACAAAAAACCCATCA
  TCGGGGGCACAGGGGGGGGGGGGGGGGCAATTTTTGGGCTTTGGCGAAATTGGGGGGGGG
  GGGGAGGGGCCGCCCCCCAGGGTTCAGGCCAATGAACGGCCCGTACTTCCCCTTGTTTTT
  GTCGGGGGGCTTGAGGATCTGAAAAAACACATGAGGGTCTCCCCCACACCCATTAGGGGC
  CCCGCTTTGTCAAACCCCCCACAATATTGGGGACCTGAAAAAATGCCCCCACCTCCCGTT
  TGGAAAAAAACTAATAGCCGTCTTCAACCCACTGGGGGGCCCCGGAAAAAAGGGCCAAAT
  CCGCCTTGGGGAAGAACTGGCCCCCCCCCCGGCCCCAAGGTAAAAAAACCCCCCGGTTGT
  TCTTCCCCACACCGGAGGTTCCTGGGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001008709
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008709.1, NP_001008709.1
• RefSeq Size: 1521 bp
• RefSeq ORF: 1026 bp
• Locus ID: 5499
• Cytogenetics: 11q13.2
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Focal adhesion, Insulin signaling pathway, Long-term potentiation, Oocyte meiosis, Regulation of actin cytoskeleton, Vascular smooth muscle contraction
• Gene Summary: 'The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). This broadly expressed gene encodes the alpha subunit of the PP1 complex that associates with over 200 regulatory proteins to form holoenzymes which dephosphorylate their biological targets with high specificity. PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies suggest that PP1 is an important regulator of cardiac function and that PP1 deregulation is implicated in diabetes and multiple types of cancer. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2020]'
  Transcript Variant: This variant (3, also called PP-1 alpha 2) uses an alternate in-frame splice site at the 5' end of an exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is longer compared to isoform 1.