SEMA4A (NM_022367) Human Untagged Clone

CAT#: SC128157

SEMA4A (untagged)-Human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A (SEMA4A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SEMA4A
• Synonyms: CORD10; RP35; SEMAB; SEMB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128157 sequence for NM_022367 edited (data generated by NextGen Sequencing)
  ATGGCCCTCCCAGCCCTGGGCCTGGACCCCTGGAGCCTCCTGGGCCTTTTCCTCTTCCAA
  CTGCTTCAGCTGCTGCTGCCGACGACGACCGCGGGGGGAGGCGGGCAGGGGCCCATGCCC
  AGGGTCAGATACTATGCAGGGGATGAACGTAGGGCACTTAGCTTCTTCCACCAGAAGGGC
  CTCCAGGATTTTGACACTCTGCTCCTGAGTGGTGATGGAAATACTCTCTACGTGGGGGCT
  CGAGAAGCCATTCTGGCCTTGGATATCCAGGATCCAGGGGTCCCCAGGCTAAAGAACATG
  ATACCGTGGCCAGCCAGTGACAGAAAAAAGAGTGAATGTGCCTTTAAGAAGAAGAGCAAT
  GAGACACAGTGTTTCAACTTCATCCGTGTCCTGGTTTCTTACAATGTCACCCATCTCTAC
  ACCTGCGGCACCTTCGCCTTCAGCCCTGCTTGTACCTTCATTGAACTTCAAGATTCCTAC
  CTGTTGCCCATCTCGGAGGACAAGGTCATGGAGGGAAAAGGCCAAAGCCCCTTTGACCCC
  GCTCACAAGCATACGGCTGTCTTGGTGGATGGGATGCTCTATTCTGGTACTATGAACAAC
  TTCCTGGGCAGTGAGCCCATCCTGATGCGCACACTGGGATCCCAGCCTGTCCTCAAGACC
  GACAACTTCCTCCGCTGGCTGCATCATGACGCCTCCTTTGTGGCAGCCATCCCTTCGACC
  CAGGTCGTCTACTTCTTCTTCGAGGAGACAGCCAGCGAGTTTGACTTCTTTGAGAGGCTC
  CACACATCGCGGGTGGCTAGAGTCTGCAAGAATGACGTGGGCGGCGAAAAGCTGCTGCAG
  AAGAAGTGGACCACCTTCCTGAAGGCCCAGCTGCTCTGCACCCAGCCGGGGCAGCTGCCC
  TTCAACGTCATCCGCCACGCGGTCCTGCTCCCCGCCGATTCTCCCACAGCTCCCCACATC
  TACGCAGTCTTCACCTCCCAGTGGCAGGTTGGCGGGACCAGGAGCTCTGCGGTTTGTGCC
  TTCTCTCTCTTGGACATTGAACGTGTCTTTAAGGGGAAATACAAAGAGTTGAACAAAGAA
  ACTTCACGCTGGACTACTTATAGGGGCCCTGAGACCAACCCCCGGCCAGGCAGTTGCTCA
  GTGGGCCCCTCCTCTGATAAGGCCCTGACCTTCATGAAGGACCATTTCCTGATGGATGAG
  CAAGTGGTGGGGACGCCCCTGCTGGTGAAATCTGGCGTGGAGTATACACGGCTTGCAGTG
  GAGACAGCCCAGGGCCTTGATGGGCACAGCCATCTTGTCATGTACCTGGGAACCACCACA
  GGGTCGCTCCACAAGGCTGTGGTAAGTGGGGACAGCAGTGCTCATCTGGTGGAAGAGATT
  CAGCTGTTCCCTGACCCTGAACCTGTTCGCAACCTGCAGCTGGCCCCCACCCAGGGTGCA
  GTGTTTGTAGGCTTCTCAGGAGGTGTCTGGAGGGTGCCCCGAGCCAACTGTAGTGTCTAT
  GAGAGCTGTGTGGACTGTGTCCTTGCCCGGGACCCCCACTGTGCCTGGGACCCTGAGTCC
  CGAACCTGTTGCCTCCTGTCTGCCCCCAACCTGAACTCCTGGAAGCAGGACATGGAGCGG
  GGGAACCCAGAGTGGGCATGTGCCAGTGGCCCCATGAGCAGGAGCCTTCGGCCTCAGAGC
  CGCCCGCAAATCATTAAAGAAGTCCTGGCTGTCCCTAACTCCATCCTGGAGCTCCCCTGC
  CCCCACCTGTCAGCCTTGGCCTCTTATTATTGGAGTCATGGCCCAGCAGCAGTCCCAGAA
  GCCTCTTCCACTGTCTACAATGGCTCCCTCTTGCTGATAGTGCAGGATGGAGTTGGGGGT
  CTCTACCAGTGCTGGGCAACTGAGAATGGCTTTTCATACCCTGTGATCTCCTACTGGGTG
  GACAGCCAGGACCAGACCCTGGCCCTGGATCCTGAACTGGCAGGCATCCCCCGGGAGCAT
  GTGAAGGTCCCGTTGACCAGGGTCAGTGGTGGGGCCGCCCTGGCTGCCCAGCAGTCCTAC
  TGGCCCCACTTTGTCACTGTCACTGTCCTCTTTGCCTTAGTGCTTTCAGGAGCCCTCATC
  ATCCTCGTGGCCTCCCCATTGAGAGCACTCCGGGCTCGGGGCAAGGTTCAGGGCTGTGAG
  ACCCTGCGCCCTGGGGAGAAGGCCCCGTTAAGCAGAGAGCAACACCTCCAGTCTCCCAAG
  GAATGCAGGACCTCTGCCAGTGATGTGGACGCTGACAACAACTGCCTAGGCACTGAGGTA
  GCTTAA
  
  Clone variation with respect to NM_022367.3
  1716 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022367 unedited
  TGTATACGACTCATATAGGCGGCCGCGAATTCGGCACGAGGCTGGCTTTGGCATGATGGG
  CACCTGGAGGGCCGCACTCCCGTTCCAGCCAGGCTGAGCCTTCTGTCCCCTGCCTCTGGG
  GCCTGGGAACCCCCCTTCTTCTTTCTCCTGAATGGCACCCCCGCCCTAGAATCCAGACAC
  CGAGTTTCCCACTGTGGCTGGTTCAAGGAGCTCCCTGGTGACAGTCTGTGGCTGAGCATG
  GCCCTCCCAGCCCTGGGCCTGGACCCCTGGAGCCTTCTGGGCCTTTTCCTCTCCCAACTG
  CTTCAGCTGCTGCTGCCGACGACCACCGCGGGGGGAGGCGGGCAGGGGCCCATGCCCAGG
  GCCATATACTATGCAGGGGATGAACGTACGGCACTTAGCTTCTTCCACCAGAAGGGCCTC
  CAGGATTTTGACACTCTGCTCCTGAGTGGNGACGGAAATACTCTCTACGTGGGGGCTCGA
  GAAGCCATTCTGGCCTTGGATATCCCCCATCCCAGGGGTCCCCAGGCTAAAGAACATGAT
  ACCGTGGCCAGCCCGTGACCGAAAAAAGAGCGAATGCGCCTTTAACACGAAAGCAATGAG
  AACACAGGTTTCACTCCATCCGCGTCCTGGCTTTCTTACAATGTCACCCATCTCTACACC
  CTGCGGAACCTTCCCCCTTAACCCTGCTTGCCCCCTTCTTGAAATTCCCGACTCCTACCT
  GGTGCCCACCTTGCAGACAATGTCACTGCGGCGAAAAGCCCAAACCCCTTTGCCCCGTTC
  CCAACCTACGGCTTGTCTCGTGGAGTGCGAGGCCCTTTTTGGGATACGAAAAATTCCTTG
  GCAGTGCGCCCTTCTGATGCCACACTGGGACCCACTCTGTCCTAAACCGCCACCTTCTCC
  CTGGCTCGATATGAACCCCCTTGGGGCAGACTCCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022367 unedited
  TANGATCCGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACCCTTATATTTTT
  AATAAAAAATAAACAGTCTCTGACAAGCAGTTTTCTGAATCCCAAAACAAAGGAAAAGGG
  AGGGGGAGAGGTGAAGGGGTCAGCTAGGGTAAAGGAGTGAAGAAGGCTCAGATTACCCCT
  GCCATTCTGCCAGGGCAGAAGGGATCAGAGTCTGCCCCAACTGAAGCAAGAAGAAAGGTG
  GTCAGACTTCAGGGAAGACTTCCTGGGAGTCAGCGGTGCACGACTGGTAAGGGAAGCAGG
  AGGGAGCAGATCCCTGCATGACCCTGGGAGAAGGGAGTGTTGGTGTCCAAAGCGGCAGCT
  TCAGAGTGGAGTTTCCAGGAGTGGCATGTTAGCATATGATTGTTTAGATGTTTGGTGTTC
  ATTACCATAGGGTCCTGGGACAGGCAGGTTTTTTAGGGTCTCTTGAAACACTGTGTTTCT
  GGAGGGTCCCTGGAATGGCCAGAACTGAAGGATCCTCTCCAGGTTCTTCAATATCAGTGT
  AGGAGCAGGTCTGGGGGTAGCCCCCACCCTGTTAGAGAGCTCATGTGCTTGGTAGAAGGG
  AGTCCCATAGGGGAGGCAGACTGCTGTGCATCACCCTGCTGAGTGTCATCAGTGATGCAG
  AGTAGCAGAAGCTCCTCTCAGGGGAGAAAGGTGGTCTTTTGTGCTGCTGTCATCCTAGTC
  AGGGCTGGGCTTGGCCCGCCCAGCCAGCATGGCCAGGTGCCTGGACCGGAACCCCGGCCT
  GGGCCTAGAAGTTTAGCTACCCCAGTGCCTAAGCCGTTGGTGTCAGCGTCCACATCACTG
  GCAGAAGTCCTGGCATCTTTGGAGACTGGG
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_022367
• ORF Size: 2286 bp
• Insert Size: 3900
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_022367.2, NP_071762.2
• RefSeq Size: 3257
• RefSeq ORF: 2286
• Locus ID: 64218
• Domains: Sema, PSI, PSI
• Protein Families: Transmembrane
• Protein Pathways: Axon guidance
• Gene Summary: This gene encodes a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is an activator of T-cell-mediated immunity and suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Mutations in this gene are associated with retinal degenerative diseases including retinitis pigmentosa type 35 (RP35) and cone-rod dystrophy type 10 (CORD10). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010]
  Transcript Variant: This variant (1) is the longest transcript. Variants 1-3 encode the same isoform (1).