DPM1 (NM_003859) Human Untagged Clone

CAT#: SC128254

DPM1 (untagged)-Human dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit (DPM1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DPM1
• Synonyms: CDGIE; MPDS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003859 edited
  ATGGCCTCCTTGGAAGTCAGTCGTAGTCCTCGCAGGTCTCGGCGGGAGCTGGAAGTGCGC
  AGTCCACGACAGAACAAATATTCGGTGCTTTTACCTACCTACAACGAGCGCGAGAACCTG
  CCGCTCATCGTGTGGCTGCTGGTGAAAAGCTTCTCCGAGAGTGGAATCAACTATGAAATT
  ATAATCATAGATGATGGAAGCCCAGATGGAACAAGGGATGTTGCTGAACAGTTGGAGAAG
  ATCTATGGGTCAGACAGAATTCTTCTAAGACCACGAGAGAAAAAGTTGGGACTAGGAACT
  GCATATATTCATGGAATGAAACATGCCACAGGAAACTACATCATTATTATGGATGCTGAT
  CTCTCACACCATCCAAAATTTATTCCTGAATTTATTAGGAAGCAAAAGGAGGGTAATTTT
  GATATTGTCTCTGGAACTCGCTACAAAGGAAATGGAGGTGTATATGGCTGGGATTTGAAA
  AGAAAAATAATCAGCCGTGGGGCCAATTTTTTAACTCAGATCTTGCTGAGACCAGGAGCA
  TCTGATTTAACAGGAAGTTTCAGATTATACCGAAAAGAAGTTCTAGAGAAATTAATAGAA
  AAATGTGTTTCTAAAGGCTACGTCTTCCAGATGGAGATGATTGTTCGGGCAAGACAGTTG
  AATTATACTATTGGCGAGGTTCCAATATCATTTGTGGATCGTGTTTATGGTGAATCCAAG
  TTGGGAGGAAATGAAATAGTATCTTTCTTGAAAGGATTATTGACTCTTTTTGCTACTACA
  TAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003859 unedited
  NGGTTAGATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGATGGCCTCC
  TTGGAAGTCAGTCGTAGTCCTCGCAGGTCTCGGCGGGAGCTGGAAGTGCGCAGTCCACGA
  CAGAACAAATATTCGGTGCTTTTACCTACCTACAACGAGCGCGAGAACCTGCCGCTCATC
  GTGTGGCTGCTGGTGAAAAGCTTCTCCGAGAGTGGAATCAACTATGAAATTATAATCATA
  GATGATGGAAGCCCAGATGGAACAAGGGATGTTGCTGAACAGTTGGAGAAGATCTATGGG
  TCAGACAGAATTCTTCTAAGACCACGAGAGAAAAAGTTGGGACTAGGAACTGCATATATT
  CATGGAATGAAACATGCCACAGGAAACTACATCATTATTATGGATGCTGATCTCTCACAC
  CATCCAAAATTTATTCCTGAATTTATTAGGAAGCAAAAGGAGGGTAATTTTGATATTGTC
  TCTGGAACTCGCTACAAAGGAAATGGAGGTGTATATGGCTGGGATTTGAAAAGAAAAATA
  ATCAGCCGTGGGGCCAATTTTTTAACTCAGATCTTGCTGAGACCAGGAGCATCTGATTTA
  ACAGGAAGTTTCAGATTATACCGAAAAGAAGTTCTAGAGAAATTAATAGAAAAATGTGTT
  TCTAAAGGCTACGTCTTCCAGATGGAGATGATTGTTCGGGCAAGACAGTTGAATTATACT
  ATTGGCGAGGTTCCAATATCATTTGTGGATCGTGTTTATGGTGAATCCAAGTTGGGAGGA
  AATGAAATAGTATCTTTCTTNGAAGGATTATTGACTCTTTTTGCTACTACATAAAAGAAA
  GATACTCATTTATAGTTACGTTCATTTCAGGTTAAACACTGAAGAAGCCTGGNTACTGAT
  TTGTAN
  
• Restriction Sites: Please inquire
• ACCN: NM_003859
• ORF Size: 783 bp
• Insert Size: 1200
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_003859.1, NP_003850.1
• RefSeq Size: 1047
• RefSeq ORF: 783
• Locus ID: 8813
• Domains: Glycos_transf_2
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. Mutations in this gene are associated with congenital disorder of glycosylation type Ie. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1.