Collagen IV (COL4A3) (NM_000091) Human Untagged Clone

CAT#: SC300008

COL4A3 (untagged)-Human collagen, type IV, alpha 3 (Goodpasture antigen) (COL4A3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COL4A3
• Synonyms: ATS2; ATS3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000091, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCGCCCGGACCGCCCCCAGGCCGCAGGTGCTCCTGCTGCCGCTCCTGCTGGTGCTCCTGGCGGCGG
  CGCCCGCAGCCAGCAAGGGTTGTGTCTGTAAAGACAAAGGCCAGTGCTTCTGTGACGGGGCCAAAGGGGA
  GAAGGGGGAGAAGGGCTTTCCTGGACCCCCCGGTTCTCCTGGCCAGAAAGGATTCACAGGTCCTGAAGGC
  TTGCCTGGACCGCAGGGACCCAAGGGCTTTCCAGGACTTCCAGGACTCACGGGTTCCAAAGGTGTAAGGG
  GAATAAGTGGATTGCCAGGATTTTCTGGTTCTCCTGGACTTCCAGGCACCCCAGGCAATACCGGGCCTTA
  CGGACTTGTCGGTGTACCAGGATGCAGTGGTTCTAAGGGTGAGCAGGGGTTTCCAGGACTCCCAGGGACA
  CTGGGCTACCCAGGGATCCCGGGTGCTGCTGGTTTGAAAGGACAAAAGGGTGCTCCTGCTAAAGAAGAAG
  ATATAGAACTTGATGCAAAAGGCGACCCCGGGTTGCCAGGGGCTCCAGGACCCCAGGGTTTGCCAGGCCC
  TCCAGGTTTTCCTGGGCCTGTTGGCCCACCTGGTCCTCCGGGATTCTTTGGCTTTCCAGGAGCCATGGGA
  CCTAGAGGACCTAAGGGTCACATGGGTGAAAGAGTGATAGGACATAAAGGAGAGCGGGGTGTGAAAGGGT
  TAACAGGACCCCCGGGACCACCAGGAACAGTTATTGTGACCCTAACTGGCCCAGATAACAGAACGGACCT
  CAAGGGGGAAAAGGGAGACAAGGGAGCAATGGGCGAGCCTGGACCTCCTGGACCCTCAGGACTGCCTGGA
  GAATCATATGGATCTGAAAAGGGTGCTCCTGGAGACCCTGGCCTGCAGGGAAAACCCGGAAAAGATGGTG
  TTCCTGGCTTCCCTGGAAGTGAGGGAGTCAAGGGCAACAGGGGTTTCCCTGGGTTAATGGGTGAAGATGG
  CATTAAGGGACAGAAAGGGGACATTGGCCCTCCAGGATTTCGTGGTCCAACAGAATATTATGACACATAC
  CAGGAAAAGGGAGATGAAGGCACTCCAGGCCCACCAGGGCCCAGAGGAGCTCGTGGCCCACAAGGTCCCA
  GTGGTCCCCCCGGAGTTCCTGGAAGTCCTGGATCATCAAGGCCTGGCCTCAGAGGAGCCCCTGGATGGCC
  AGGCCTGAAAGGAAGTAAAGGGGAACGAGGCCGCCCAGGAAAGGATGCCATGGGGACTCCTGGGTCCCCA
  GGTTGTGCTGGTTCACCAGGTCTTCCAGGATCACCGGGACCTCCAGGACCGCCAGGTGACATCGTTTTTC
  GCAAGGGTCCACCTGGAGATCACGGACTGCCAGGCTATCTAGGGTCTCCAGGAATCCCAGGAGTTGATGG
  GCCCAAAGGAGAACCAGGCCTCCTGTGTACACAGTGCCCTTATATCCCAGGGCCTCCCGGTCTCCCAGGA
  TTGCCAGGGTTACATGGTGTAAAAGGAATCCCAGGAAGACAAGGCGCAGCTGGCTTGAAAGGAAGCCCAG
  GGTCCCCAGGAAATACAGGTCTTCCAGGATTTCCAGGTTTCCCAGGTGCCCAGGGTGACCCAGGACTTAA
  AGGAGAAAAAGGTGAAACACTTCAGCCTGAGGGGCAAGTGGGTGTCCCAGGTGACCCGGGGCTCAGAGGC
  CAACCTGGGAGAAAGGGCTTGGATGGAATTCCTGGAACTCCGGGAGTGAAAGGATTACCAGGACCTAAAG
  GCGAACTGGCTCTGAGTGGTGAGAAAGGGGACCAAGGTCCTCCAGGGGATCCTGGCTCCCCTGGGTCCCC
  AGGACCTGCAGGACCAGCTGGACCACCTGGCTACGGACCCCAAGGAGAACCTGGTCTCCAGGGCACGCAA
  GGAGTTCCTGGAGCCCCCGGACCACCCGGAGAAGCCGGCCCTAGGGGAGAGCTCAGTGTTTCAACACCAG
  TTCCAGGCCCACCAGGACCTCCAGGGCCCCCTGGCCATCCTGGCCCCCAAGGTCCACCTGGTATCCCTGG
  ATCCCTGGGGAAATGTGGAGATCCTGGTCTTCCAGGGCCTGATGGTGAACCAGGAATTCCAGGAATTGGA
  TTTCCTGGGCCTCCTGGACCTAAGGGAGACCAAGGTTTTCCAGGTACAAAAGGATCACTGGGTTGTCCTG
  GAAAAATGGGAGAGCCTGGGTTACCTGGAAAGCCAGGCCTCCCAGGAGCCAAGGGAGAACCAGCAGTAGC
  CATGCCTGGAGGACCAGGAACACCAGGTTTTCCAGGAGAAAGAGGCAATTCTGGGGAACATGGAGAAATT
  GGACTCCCTGGACTTCCAGGTCTCCCTGGAACTCCAGGAAATGAAGGGCTTGATGGACCACGAGGAGATC
  CAGGGCAGCCTGGACCACCTGGAGAACAAGGACCCCCAGGAAGGTGCATAGAGGGTCCCAGGGGAGCCCA
  AGGACTTCCAGGCTTAAATGGATTGAAAGGGCAACAAGGCAGAAGAGGTAAAACGGGGCCAAAGGGAGAC
  CCAGGAATTCCAGGCTTGGATAGATCAGGATTTCCTGGAGAAACTGGATCACCAGGAATTCCAGGTCATC
  AAGGTGAAATGGGACCACTGGGTCAAAGAGGATATCCAGGAAATCCGGGAATTTTAGGGCCACCAGGTGA
  AGATGGAGTGATTGGGATGATGGGCTTTCCTGGAGCCATTGGCCCTCCAGGGCCCCCTGGGAACCCAGGC
  ACACCAGGGCAGAGGGGGAGCCCTGGAATTCCAGGAGTAAAGGGCCAGAGAGGAACCCCAGGAGCCAAGG
  GGGAACAAGGAGATAAAGGAAATCCCGGGCCTTCAGAGATATCCCACGTAATAGGGGACAAAGGAGAACC
  AGGTCTCAAAGGATTCGCAGGAAATCCAGGTGAGAAAGGAAACAGAGGCGTTCCAGGGATGCCAGGTTTA
  AAGGGCCTCAAAGGACTACCCGGACCAGCAGGACCACCAGGCCCCAGAGGAGATTTGGGCAGCACTGGGA
  ATCCTGGAGAACCAGGACTGCGTGGTATACCAGGAAGCATGGGGAACATGGGCATGCCAGGTTCTAAAGG
  AAAAAGGGGAACTTTGGGATTCCCAGGTCGAGCAGGAAGACCAGGCCTCCCAGGTATTCATGGTCTCCAG
  GGAGATAAGGGAGAGCCAGGTTATTCAGAAGGTACAAGGCCAGGACCACCGGGACCAACGGGGGATCCAG
  GACTGCCGGGTGATATGGGAAAGAAAGGAGAAATGGGGCAACCTGGCCCACCTGGACATTTGGGGCCTGC
  TGGACCTGAGGGAGCCCCTGGAAGTCCTGGAAGTCCTGGCCTCCCAGGAAAGCCAGGTCCTCATGGTGAT
  TTGGGTTTTAAAGGAATCAAAGGCCTCCTGGGCCCTCCAGGAATCAGAGGCCCTCCAGGTCTTCCAGGAT
  TTCCAGGATCTCCTGGACCAATGGGTATAAGAGGTGACCAAGGACGTGATGGAATTCCTGGTCCAGCCGG
  AGAAAAGGGAGAAACGGGTTTATTGAGGGCCCCTCCAGGCCCAAGAGGGAACCCTGGTGCTCAAGGAGCC
  AAAGGAGACAGGGGAGCCCCAGGTTTTCCTGGCCTCCCGGGCAGAAAAGGGGCCATGGGAGATGCTGGAC
  CTCGAGGACCCACAGGCATAGAAGGATTCCCAGGGCCACCAGGTCTGCCCGGTGCAATTATCCCTGGCCA
  GACAGGAAATCGTGGTCCACCAGGCTCAAGAGGAAGCCCAGGTGCGCCTGGTCCCCCTGGACCTCCAGGG
  AGTCATGTAATAGGCATAAAAGGAGACAAAGGGTCTATGGGCCACCCTGGCCCAAAAGGTCCACCTGGAA
  CTGCAGGAGACATGGGACCACCAGGTCGTCTGGGAGCACCAGGTACTCCAGGTCTTCCAGGACCCAGAGG
  TGATCCTGGATTCCAGGGGTTTCCAGGCGTGAAAGGAGAAAAGGGTAATCCTGGATTTCTAGGATCCATT
  GGACCTCCAGGACCAATTGGGCCAAAAGGACCACCTGGTGTACGTGGAGACCCTGGCACACTTAAGATTA
  TCTCCCTTCCAGGAAGCCCAGGGCCACCTGGCACACCTGGAGAACCAGGGATGCAGGGAGAACCTGGGCC
  ACCAGGGCCACCTGGAAACCTAGGACCCTGTGGGCCAAGAGGTAAGCCAGGCAAGGATGGAAAACCAGGA
  ACTCCTGGACCAGCTGGAGAAAAAGGCAACAAAGGTTCTAAAGGAGAGCCAGGACCAGCTGGATCAGATG
  GATTGCCAGGTTTGAAAGGAAAACGTGGAGACAGTGGATCACCTGCAACCTGGACAACGAGAGGCTTTGT
  CTTCACCCGACACAGTCAAACCACAGCAATTCCTTCATGTCCAGAGGGGACAGTGCCACTCTACAGTGGG
  TTTTCTTTTCTTTTTGTACAAGGAAATCAACGAGCCCACGGACAAGACCTTGGAACTCTTGGCAGCTGCC
  TGCAGCGATTTACCACAATGCCATTCTTATTCTGCAATGTCAATGATGTATGTAATTTTGCATCTCGAAA
  TGATTATTCATACTGGCTGTCAACACCAGCTCTGATGCCAATGAACATGGCTCCCATTACTGGCAGAGCC
  CTTGAGCCTTATATAAGCAGATGCACTGTTTGTGAAGGTCCTGCGATCGCCATAGCCGTTCACAGCCAAA
  CCACTGACATTCCTCCATGTCCTCACGGCTGGATTTCTCTCTGGAAAGGATTTTCATTCATCATGTTCAC
  AAGTGCAGGTTCTGAGGGCACCGGGCAAGCACTGGCCTCCCCTGGCTCCTGCCTGGAAGAATTCCGAGCC
  AGCCCATTTCTAGAATGTCATGGAAGAGGAACGTGCAACTACTATTCAAATTCCTACAGTTTCTGGCTGG
  CTTCATTAAACCCAGAAAGAATGTTCAGAAAGCCTATTCCATCAACTGTGAAAGCTGGGGAATTAGAAAA
  AATAATAAGTCGCTGTCAGGTGTGCATGAAGAAAAGACACTGA
  
  
• Restriction Sites: AscI-MluI
• ACCN: NM_000091
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000091.4, NP_000082.2
• RefSeq Size: 8114 bp
• RefSeq ORF: 5013 bp
• Locus ID: 1285
• Cytogenetics: 2q36.3
• Protein Families: Druggable Genome
• Gene Summary: 'Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1.