PDE6 alpha (PDE6A) (NM_000440) Human Untagged Clone

CAT#: SC300070

PDE6A (untagged)-Human phosphodiesterase 6A, cGMP-specific, rod, alpha (PDE6A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE6A
• Synonyms: CGPR-A; PDEA; RP43
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000440 edited
  TTGCAGACTGCAAAACTGCCATTGGAAGGCCTCCGTCCCAGTCCTTCTACAGAGTAGCCA
  GTGGGATTCCCAGCCATGGGCGAGGTGACAGCAGAGGAGGTGGAGAAGTTCCTGGACTCG
  AATATTGGCTTTGCCAAACAGTACTACAACCTCCACTACCGGGCCAAGCTCATCTCCGAC
  CTCCTTGGGGCCAAGGAGGCTGCCGTGGACTTCAGCAACTACCACTCCCCGAGCAGCATG
  GAGGAGAGCGAAATCATCTTTGATCTCCTGCGGGACTTTCAGGAGAATTTACAGACAGAG
  AAATGCATCTTCAATGTCATGAAGAAGCTGTGCTTCCTCCTGCAGGCAGACCGCATGAGC
  CTGTTCATGTACCGGACCCGCAATGGCATCGCAGAGCTGGCCACCAGGCTTTTCAATGTC
  CACAAGGATGCTGTCCTCGAGGACTGCCTGGTGATGCCCGACCAAGAGATCGTCTTCCCT
  TTGGACATGGGCATCGTGGGCCATGTCGCACACTCTAAGAAGATTGCTAACGTCCCCAAC
  ACAGAGGAGGATGAGCATTTCTGTGACTTTGTGGACATCCTCACAGAGTACAAGACCAAG
  AACATCTTGGCTTCCCCCATAATGAATGGGAAGGATGTGGTGGCCATAATCATGGCTGTG
  AATAAAGTGGATGGATCCCACTTCACCAAGAGAGATGAAGAGATTCTTCTCAAGTACCTC
  AATTTTGCAAATCTAATCATGAAGGTGTACCACCTGAGTTACCTGCACAACTGTGAAACT
  CGACGTGGCCAGATACTGCTGTGGTCTGGGAGCAAAGTCTTTGAAGAACTTACGGACATC
  GAACGACAGTTCCACAAAGCCCTGTACACAGTCCGTGCTTTCCTCAACTGTGACAGATAC
  TCTGTGGGTCTCTTAGACATGACCAAGCAGAAGGAATTTTTTGATGTGTGGCCGGTTCTG
  ATGGGTGAAGTTCCACCTTACTCTGGTCCCAGGACTCCGGATGGAAGAGAAATTAACTTT
  TACAAGGTCATTGACTACATCCTGCATGGCAAAGAGGACATCAAAGTCATCCCGAATCCA
  CCTCCTGACCATTGGGCTTTAGTAAGCGGTCTCCCAGCTTATGTTGCCCAGAATGGCCTG
  ATTTGCAACATCATGAACGCGCCTGCGGAGGACTTTTTTGCATTTCAGAAAGAACCTCTG
  GATGAGTCTGGATGGATGATTAAAAATGTGCTTTCAATGCCGATTGTGAACAAGAAGGAA
  GAAATTGTTGGAGTGGCCACATTTTACAATCGTAAAGATGGGAAGCCCTTTGATGAAATG
  GATGAGACGCTCATGGAGTCTTTGACTCAATTTCTGGGCTGGTCTGTCTTAAATCCTGAC
  ACCTATGAGTCAATGAATAAACTTGAAAATAGGAAGGATATTTTCCAGGACATAGTAAAA
  TATCATGTGAAGTGTGACAATGAAGAAATTCAGAAAATCTTGAAAACCAGAGAGGTGTAT
  GGGAAGGAGCCATGGGAGTGTGAGGAAGAGGAGCTGGCTGAGATCCTGCAAGCGGAGCTG
  CCAGATGCAGATAAATACGAAATTAATAAATTTCACTTCAGTGACTTACCCCTAACAGAA
  CTGGAGCTGGTAAAATGTGGAATACAGATGTATTATGAGCTCAAAGTGGTGGATAAATTT
  CACATTCCACAAGAGGCCCTGGTGCGGTTCATGTACTCCCTGAGTAAGGGCTACCGCAAG
  ATCACCTACCACAACTGGCGGCACGGCTTCAACGTGGGGCAGACCATGTTCTCCCTGCTG
  GTGACGGGAAAGCTGAAGCGCTACTTCACGGACCTAGAGGCCTTGGCCATGGTCACTGCT
  GCTTTCTGCCATGACATTGACCACAGAGGCACCAATAACCTCTACCAGATGAAATCCCAG
  AACCCACTGGCCAAGCTCCATGGGTCCTCTATCTTGGAAAGACACCACTTGGAGTTTGGC
  AAAACACTGCTCAGAGACGAGAGCCTGAATATCTTTCAAAACCTCAATCGTCGACAGCAT
  GAGCATGCCATCCACATGATGGACATTGCAATCATTGCCACAGACCTCGCCCTGTATTTC
  AAGAAGAGGACGATGTTCCAAAAGATCGTGGATCAGTCTAAGACATATGAGAGTGAACAG
  GAGTGGACACAGTACATGATGCTGGAGCAGACACGGAAGGAAATCGTTATGGCCATGATG
  ATGACCGCCTGTGATCTCTCAGCCATCACCAAACCCTGGGAGGTGCAGAGCCAGGTAGCT
  CTGCTGGTGGCTGCTGAATTCTGGGAACAAGGTGACCTGGAGCGCACGGTGCTGCAACAG
  AATCCCATTCCCATGATGGACAGAAACAAAGCAGATGAACTCCCTAAGCTTCAAGTCGGC
  TTCATTGACTTTGTTTGCACCTTCGTCTACAAGGAATTCTCCCGTTTCCACGAGGAGATC
  ACCCCAATGTTGGACGGGATCACCAACAATCGCAAGGAGTGGAAGGCGCTTGCTGATGAG
  TACGATGCCAAGATGAAGGTGCAGGAGGAGAAGAAGCAGAAACAGCAGTCGGCCAAGTCA
  GCAGCCGCAGGAAATCAGCCGGGGGGAAACCCCAGCCCAGGGGGTGCAACTACATCCAAG
  TCCTGCTGCATCCAGTAACACCACTGGGGATGTGCTGGCTGGACGGCACCACCCTTTCCT
  GGGAAGAGATGACTCAAGCCAGTGGAAGACCACACACCTTGAGAAGTAGAAGAGTCATAG
  GATTTGAAAGCTGTTAGAGAATTTAGCTTCCAGGACTGTTCAATCTTTTGGCTTCCCTGG
  GCCACATTGGAAGAATTGTCTTGGGTCACACATAAAATACAGTAACACTAATGATAGCTG
  TTGAACTTAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000440 unedited
  AGGGGAGGGGGGNNNGGGGGGGTCATATTTGTATACGACTCACTATAGGCGGCCGCGAAT
  TCGGCACGAGGTTGCAGACTGCAAAACTGCCATTGGAAGGCCTCCGTCCCAGTCCTTCTA
  CAGAGTAGCCAGTGGGATTCCCAGCCATGGGCGAGGTGACAGCAGAGGAGGTGGAGAAGT
  TCCTGGACTCGAATATTGGCTTTGCCAAACAGTACTACAACCTCCACTACCGGGCCAAGC
  TCATCTCCGACCTCCTTGGGGCCAAGGAGGCTGCCGTGGACTTCAGCAACTACCACTCCC
  CGAGCAGCATGGAGGAGAGCGAAATCATCTTTGATCTCCTGCGGGACTTTCAGGAGAATT
  TACAGACAGAGAAATGCATCTTCAATGTCATGAAGAAGCTGTGCTTCCTCCTGCAGGCAG
  ACCGCATGAGCCTGTTCATGTACCGGACCCGCAATGGCATCGCAGAGCTGGCCACCAGGC
  TTTTCAATGTCCACAAGGATGCTGTCCTCGAGGACTGCCTGGTGATGCCCGACCAAGAGA
  TCGTCTTCCCTTTGGACATGGGCATCGTGGGCCATGTCGCACACTCTAAGAAGATTGCTA
  ACGTCCCCAACACAGAGGAGGATGAGCATTTCTGTGACTTTGTGGACATCCTCACAGAGT
  ACAAGACCAAGAACATCTTGGCTTCCCCCATAATGAATGGGAAGGATGTGGTGGCCATAA
  TCATGGCTGTGAATAAAGTGGATGGATCCCACTTCACCAAGAGAGATGAAGAGATTCTTC
  TCAAGTACCTCAATTNTGCAAATCTAATCATGAAGGTGTACCACCTGAGTTACCTGCANC
  ACTGTGAAACTCGACGTGGCCAGATACTGCTGTGGTCTGGGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000440 unedited
  NGGGTTTTGNNNNNNNNCCCGCCTTGGNNGATGGCACTTNCCAGGNCCAGNAAAGCACTG
  GGGNAGGGTCACAGGGNTGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCG
  CAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTAAGTTCAACAGCTATCATTAGTGTTA
  CTGTATTTTATGTGTGACCCAAGACAATTCTTCCAATGTGGCCCAGGGAAGCCAAAAGAT
  TGAACAGTCCTGGAAGCTAAATTCTCTAACAGCTTTCAAATCCTATGACTCTTCTACTTC
  TCAAGGTGTGTGGTCTTCCACTGGCTTGAGTCATCTCTTCCCAGGAAAGGGTGGTGCCGT
  CCAGCCAGCACATCCCCAGTGGTGTTACTGGATGCAGCAGGACTTGGATGTAGTTGCACC
  CCCTGGGCTGGGGTTTCCCCCCGGCTGATTTCCTGCGGCTGCTGACTTGGCCGACTGCTG
  TTTCTGCTTCTTCTCCTCCTGCACCTTCATCTTGGCATCGTACTCATCAGCAAGCGCCTT
  CCACTCCTTGCGATTGTTGGTGATCCCGTCCAACATTGGGGTGATCTCCTCGTGGAAACG
  GGAGAATTCCTTGTAGACGAAGGTGCAAACAAAGTCAATGAAGCCGACTTGAAGCTTAGG
  GAGTTCATCTGCTTTGTTTCTGTCCATCATGGGAATGGGATTCTGTTGCAGCACCGTGCG
  CTCCANGTCACCTTGTTCCCAGATTTCAGCAGCCACCAGCAGAGCTACCTGGCTCTGCAC
  CTCCCAGGNTTTGGTGATGGCTGAGAGATCACAGGCGGTCATCATCATGGCCATAACGAT
  TTCCTTCCGTGTCTGCTNCAGCATCATGTACTGTGTCCACTCCTGGTCACTCTCATATGA
  
• Restriction Sites: Please inquire
• ACCN: NM_000440
• ORF Size: 2583 bp
• Insert Size: 3000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: There is 1 nucleotide difference between the OriGene clone and the NCBI reference ORF. These result in the substitution of 1 aa.

Reference Data

• RefSeq: NM_000440.1, NP_000431.1
• RefSeq Size: 2944
• RefSeq ORF: 2583
• Locus ID: 5145
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A alpha subunit, expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa. [provided by RefSeq, Jul 2008]