HTR2B (NM_000867) Human Untagged Clone

CAT#: SC300142

HTR2B (untagged)-Human 5-hydroxytryptamine (serotonin) receptor 2B (HTR2B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HTR2B
• Synonyms: 5-HT(2B); 5-HT-2B; 5-HT2B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000867 edited
  GGGGGTATTTGTTTCACTGCTTTCAACCGCCTGTGCTGGAGGCTCAGAATAAGTCAATGG
  GAGGAGGATTTCAGTCACAGCAGCAAGCAAGTCTAGTGAACAGATAAGATGACATGCTCA
  GCAAAATAACAACGAAACCAGAGGGGGAACTCTCTGGCATGCAAGTTCAAACACGACTCT
  ACAACTACGGCAGAAAAAGAGAGAGAGAGAAACTAAAAATATATATATATCCTATTTTTT
  TCACAGCTATCAGTTTCTTTCACTGAGCTTTCCTAAATTTAAGCCTCTAGAAAATAATAA
  ATACTTGGATATCTTACCTACAAACATGGACAGATGTGTGTATGCGCTCATTTTAGAGAA
  CTTGAATTTTTTTTTTTAAAGGAAGGTGTCAACTTTGGCTTTTGAGTGTTTGGCATGGTT
  ACAATGCCTTAAAAAAACAGATGAGCAGCTTAGCTACTAACCATGCTGACCACTGTTCGG
  AACGGGATTGAATCACAGAAAAACAGCAAATGGCTCTCTCTTACAGAGTGTCTGAACTTC
  AAAGCACAATTCCTGAGCACATTTTGCAGAGCACCTTTGTTCACGTTATCTCTTCTAACT
  GGTCTGGATTACAGACAGAATCAATACCAGAGGAAATGAAACAGATTGTTGAGGAACAGG
  GAAATAAACTGCACTGGGCAGCTCTTCTGATACTCATGGTGATAATACCCACAATTGGTG
  GAAATACCCTTGTTATTCTGGCTGTTTCACTGGAGAAGAAGCTGCAGTATGCTACTAATT
  ACTTTCTAATGTCCTTGGCGGTGGCTGATTTGCTGGTTGGATTGTTTGTGATGCCAATTG
  CCCTCTTGACAATAATGTTTGAGGCTATGTGGCCCCTCCCACTTGTTCTATGTCCTGCCT
  GGTTATTTCTTGACGTTCTCTTTTCAACCGCATCCATCATGCATCTCTGTGCCATTTCAG
  TGGATCGTTACATAGCCATCAAAAAGCCAATCCAGGCCAATCAATATAACTCACGGGCTA
  CAGCATTCATCAAGATTACAGTGGTGTGGTTAATTTCAATAGGCATTGCCATTCCAGTCC
  CTATTAAAGGGATAGAGACTGATGTGGACAACCCAAACAATATCACTTGTGTGCTGACAA
  AGGAACGTTTTGGCGATTTCATGCTCTTTGGCTCACTGGCTGCCTTCTTCACACCTCTTG
  CAATTATGATTGTCACCTACTTTCTCACTATCCATGCTTTACAGAAGAAGGCTTACTTAG
  TCAAAAACAAGCCACCTCAACGCCTAACATGGTTGACTGTGTCTACAGTTTTCCAAAGGG
  ATGAAACACCTTGCTCGTCACCGGAAAAGGTGGCAATGCTGGATGGTTCTCGAAAGGACA
  AGGCTCTGCCCAACTCAGGTGATGAAACACTTATGCGAAGAACATCCACAATTGGGAAAA
  AGTCAGTGCAGACCATTTCCAACGAACAGAGAGCCTCAAAGGTCCTAGGGATTGTGTTTT
  TCCTCTTTTTGCTTATGTGGTGTCCCTTCTTTATTACAAATATAACTTTAGTTTTATGTG
  ATTCCTGTAACCAAACTACTCTCCAAATGCTCCTGGAGATATTTGTGTGGATAGGCTATG
  TTTCCTCAGGAGTGAATCCTTTGGTCTACACCCTCTTCAATAAGACATTTCGGGATGCAT
  TTGGCCGATATATCACCTGCAATTACCGGGCCACAAAGTCAGTAAAAACTCTCAGAAAAC
  GCTCCAGTAAGATCTACTTCCGGAATCCAATGGCAGAGAACTCTAAGTTTTTCAAGAAAC
  ATGGAATTCGAAATGGGATTAACCCTGCCATGTACCAGAGTCCAATGAGGCTCCGAAGTT
  CAACCATTCAGTCTTCATCAATCATTCTACTAGATACGCTTCTCCTCACTGAAAATGAAG
  GTGACAAAACTGAAGAGCGAGTTAGTTATGTATAGCAGAACTGGCAGTTGTCATCAAACA
  TAATGATGAGTAAGATGATGAATGAGATGTAAATGTGCCAAGAATATATTATATAAAGAA
  TTTTATGTCATATATCAAATCATCTCTTTAACCTAAGATGTAAGTATTAAGAATATCTAA
  TTTTCCTAATTTGGACAAGATTATTCCATGAGGAAAATAATTTTATATAGCTACAAATGA
  AAACAATCCAGCACTCTGGTTAAATTTTAAGGTATTCGAATGAAATAAAGTCAAATCAAT
  AAATTTCAGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_000867
• Insert Size: 2300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone is found to be a perfect match to NM_000867.2.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000867.2, NP_000858.2
• RefSeq Size: 2260 bp
• RefSeq ORF: 1446 bp
• Locus ID: 3357
• Cytogenetics: 2q37.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway, Gap junction, Neuroactive ligand-receptor interaction
• Gene Summary: 'This gene encodes one of the several different receptors for 5-hydroxytryptamine (serotonin) that belongs to the G-protein coupled receptor 1 family. Serotonin is a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. Serotonin receptors mediate many of the central and peripheral physiologic functions of serotonin, including regulation of cardiovascular functions and impulsive behavior. Population and family-based analyses of a minor allele (glutamine-to-stop substitution, designated Q20*) which blocks expression of this protein, and knockout studies in mice, suggest a role for this gene in impulsivity. However, other factors, such as elevated testosterone levels, may also be involved. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2016]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).