GRK4 (NM_001004056) Human Untagged Clone

CAT#: SC300592

GRK4 (untagged)-Human G protein-coupled receptor kinase 4 (GRK4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRK4
• Synonyms: GPRK2L; GPRK4; GRK4a; IT11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001004056, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCTCGAGAACATCGTGGCCAACTCGCTGCTGCTGAAAGCGCGTCAAGAAAAGGATTATAGCAGTC
  TTTGTGACAAGCAACCGATAGGAAGACGTCTCTTCAGGCAGTTCTGTGATACCAAACCCACTCTAAAGAG
  GCACATTGAATTCTTGGATGCAGTGGCAGAATATGAAGTTGCCGATGATGAGGACCGAAGTGATTGTGGA
  CTGTCAATCTTAGATAGATTCTTCAATGATAAGTTGGCAGCCCCTTTACCAGAAATACCTCCAGATGTTG
  TGACAGAATGTAGATTGGGACTGAAGGAGGAGAACCCTTCCAAAAAAGCCTTTGAGGAATGTACTAGAGT
  TGCCCATAACTACCTAAGAGGGGAACCATTTGAAGAATACCAAGAAAGCTCATATTTTTCTCAGTTTTTA
  CAATGGAAATGGCTGGAAAGGCAACCCGTAACAAAGAACACATTTAGACATTACAGAGTTCTAGGAAAAG
  GCGGATTTGGAGAGGTTTGCGCCTGTCAAGTGCGAGCCACAGGAAAAATGTATGCCTGCAAAAAGCTACA
  AAAAAAAAGAATAAAGAAGAGGAAAGGTGAAGCTATGGCTCTAAATGAGAAAAGAATTCTGGAGAAAGTG
  CAAAGTAGATTCGTAGTTAGTTTAGCCTACGCTTATGAAACCAAAGATGCCTTGTGCTTGGTGCTCACCA
  TTATGAATGGAGGGGATTTGAAGTTTCACATTTACAACCTGGGCAATCCCGGCTTTGATGAGCAGAGAGC
  CGTTTTCTATGCTGCAGAGCTGTGTTGCGGCTTGGAAGATTTACAGAGGGAAAGAATTGTATACAGAGAC
  TTGAAGCCTGAGAATATTCTCCTTGATGATCGTGGACACATCCGGATTTCAGACCTCGGTTTGGCCACAG
  AGATCCCAGAAGGACAGAGGGTTCGAGGAAGAGTTGGAACAGTCGGCTACATGGCACCTGAAGTTGTCAA
  TAATGAAAAGTATACGTTTAGTCCCGATTGGTGGGGACTTGGCTGTCTGATCTATGAAATGATTCAGGGA
  CATTCTCCATTCAAAAAATACAAAGAGAAAGTCAAATGGGAGGAGGTCGATCAAAGAATCAAGAATGATA
  CCGAGGAGTATTCTGAGAAGTTTTCAGAGGATGCCAAATCTATCTGCAGGATGTTACTCACCAAGAATCC
  AAGCAAGCGGCTGGGCTGCAGGGGCGAGGGAGCGGCTGGGGTGAAGCAGCACCCCGTGTTCAAGGACATC
  AACTTCAGGAGGCTGGAGGCAAACATGCTGGAGCCCCCTTTCTGTCCTGATCCTCATGCCGTTTACTGTA
  AGGACGTCCTGGATATCGAGCAGTTCTCGGTGGTGAAAGGGATCTACCTGGACACCGCAGATGAAGACTT
  CTATGCTCGGTTTGCTACCGGGTGTGTCTCCATCCCCTGGCAGAATGAGATGATCGAATCTGGGTGTTTC
  AAAGACATCAACAAAAGTGAAAGTGAGGAAGCTTTGCCATTAGATCTAGACAAGAACATACATACCCCGG
  TTTCCAGACCAAACAGAGGCTTCTTCTATAGACTCTTCAGAAGAGGGGGCTGCCTGACCATGGTCCCCAG
  TGAGAAGGAAGTGGAACCCAAGCAATGCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001004056
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004056.1, NP_001004056.1
• RefSeq Size: 2225 bp
• RefSeq ORF: 1641 bp
• Locus ID: 2868
• Cytogenetics: 4p16.3
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis
• Gene Summary: 'This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (beta) has the same N- and C-termini but is shorter compared to isoform alpha.