GRK6 (NM_001004105) Human Untagged Clone

CAT#: SC300601

GRK6 (untagged)-Human G protein-coupled receptor kinase 6 (GRK6), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRK6
• Synonyms: GPRK6
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001004105, the custom clone sequence may differ by one or more nucleotides
  ATGGAGCTCGAGAACATCGTAGCGAACACGGTGCTACTCAAGGCCCGGGAAGGTGGCGGT
  GGAAATCGCAAAGGCAAAAGCAAGAAATGGCGGCAGATGCTCCAGTTCCCTCACATCAGC
  CAGTGCGAAGAGCTGCGGCTCAGCCTCGAGCGTGACTATCACAGCCTGTGCGAGCGGCAG
  CCCATTGGGCGCCTGCTGTTCCGAGAGTTCTGTGCCACGAGGCCGGAGCTGAGCCGCTGC
  GTCGCCTTCCTGGATGGGGTGGCCGAGTATGAAGTGACCCCGGATGACAAGCGGAAGGCA
  TGTGGGCGGCAGCTAACGCAGAATTTTCTGAGCCACACGGGTCCTGACCTCATCCCTGAG
  GTCCCCCGGCAGCTGGTGACGAACTGCACCCAGCGGCTGGAGCAGGGTCCCTGCAAAGAC
  CTTTTCCAGGAACTCACCCGGCTGACCCACGAGTACCTGAGCGTGGCCCCTTTTGCCGAC
  TACCTCGACAGCATCTACTTCAACCGTTTCCTGCAGTGGAAGTGGCTGGAAAGGCAGCCA
  GTGACCAAAAACACCTTCAGGCAATACCGAGTCCTGGGCAAAGGTGGCTTTGGGGAGGTG
  TGCGCCTGCCAGGTGCGGGCCACAGGTAAGATGTATGCCTGCAAGAAGCTAGAGAAAAAG
  CGGATCAAGAAGCGGAAAGGGGAGGCCATGGCGCTGAACGAGAAGCAGATCCTGGAGAAA
  GTGAACAGTAGGTTTGTAGTGAGCTTGGCCTACGCCTATGAGACCAAGGACGCGCTGTGC
  CTGGTGCTGACACTGATGAACGGGGGCGACCTCAAGTTCCACATCTACCACATGGGCCAG
  GCTGGCTTCCCCGAAGCGCGGGCCGTCTTCTACGCCGCCGAGATCTGCTGTGGCCTGGAG
  GACCTGCACCGGGAGCGCATCGTGTACAGGGACCTGAAGCCCGAGAACATCTTGCTGGAT
  GACCACGGCCACATCCGCATCTCTGACCTGGGACTAGCTGTGCATGTGCCCGAGGGCCAG
  ACCATCAAAGGGCGTGTGGGCACCGTGGGTTACATGGCTCCGGAGGTGGTGAAGAATGAA
  CGGTACACGTTCAGCCCTGACTGGTGGGCGCTCGGCTGCCTCCTGTACGAGATGATCGCA
  GGCCAGTCGCCCTTCCAGCAGAGGAAGAAGAAGATCAAGCGGGAGGAGGTGGAGCGGCTG
  GTGAAGGAGGTCCCCGAGGAGTATTCCGAGCGCTTTTCCCCGCAGGCCCGCTCACTTTGC
  TCACAGCTCCTCTGCAAGGACCCTGCCGAACGCCTGGGGTGTCGTGGGGGCAGTGCCCGC
  GAGGTGAAGGAGCACCCCCTCTTTAAGAAGCTGAACTTCAAGCGGCTGGGAGCTGGCATG
  CTGGAGCCGCCGTTCAAGCCTGACCCCCAGGCCATTTACTGCAAGGATGTTCTGGACATT
  GAACAGTTCTCTACGGTCAAGGGCGTGGAGCTGGAGCCTACCGACCAGGACTTCTACCAG
  AAGTTTGCCACAGGCAGTGTGCCCATCCCCTGGCAGAACGAGATGGTGGAGACCGAGTGC
  TTCCAAGAGCTGAATGTCTTTGGGCTGGATGGCTCAGTTCCCCCAGACCTGGACTGGAAG
  GGCCAGCCACCTGCACCTCCTAAAAAGGGACTGCTGCAGAGACTCTTCAGTCGCCAAAGG
  TGA
  
• Restriction Sites: Please inquire
• ACCN: NM_001004105
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004105.1, NP_001004105.1
• RefSeq Size: 1925 bp
• RefSeq ORF: 1683 bp
• Locus ID: 2870
• Cytogenetics: 5q35.3
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis
• Gene Summary: 'This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) contains an alternate 3' terminal exon, compared to variant 2, resulting in a shorter isoform (C) with a distinct C-terminus compared to isoform B.