H3C15 (NM_001005464) Human Untagged Clone

CAT#: SC300953

HIST2H3A (untagged)-Human histone cluster 2, H3a (HIST2H3A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: H3C15
• Synonyms: H3/n; H3/o; H3C13; H3C14; HIST2H3A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001005464 edited
  CGCCGCTGCGCTGGTAAGCCTGTGTTTTGGTTCGCTATGGCCCGTACTAAGCAGACTGCT
  CGCAAGTCGACCGGCGGCAAGGCCCCGAGGAAGCAGCTGGCCACCAAGGCGGCCCGCAAG
  AGCGCGCCGGCCACGGGCGGGGTGAAGAAGCCGCACCGCTACCGGCCCGGCACCGTAGCC
  CTGCGGGAGATCCGGCGCTACCAGAAGTCCACGGAGCTGCTGATCCGCAAGCTGCCCTTC
  CAGCGGCTGGTACGCGAGATCGCGCAGGACTTTAAGACGGACCTGCGCTTCCAGAGCTCG
  GCCGTGATGGCGCTGCAGGAGGCCAGCGAGGCCTACCTGGTGGGGCTGTTCGAAGACACG
  AACCTGTGCGCCATCCACGCCAAGCGCGTGACCATTATGCCCAAGGACATCCAGCTGGCC
  CGCCGCATCCGTGGAGAGCGGGCTTAAGAAGTGGCGGTTCGGCCGGAGGTTCCATCGTAT
  CCAAAAGGCTCTTTTCAGAGCCACCCACATCAGCACTTGGAAGAAGCTGTACCGCTTGCC
  CTCCGTGCTCCTCCGGCATTAGAGCGGGGAAGGCACTTCCGCTTAGGCTCCCAACAAAAC
  GGGGTCGGGTCCCCCTGCTGCGGTGCCGTCTTGAGCTTTGCCGACTTCGGCCAATTGGTC
  TATCCGCACTGGCCGGGCTGCGGACGCTTTCCTGGGCGCCGGCTCGCGGGCTTTGGCGGT
  CCGGCTCCCCCCGCGCGTGACCGGGGCCCTGCGTGGCGGGGGGGCCGGGGCAGGAGAGAC
  GGGGCGTCGGGCACGAGGCCAGGGGTCACTAGGGGGACGCTCAGGGCTCGCGGTCCTGAG
  CGCGGCTGGCCTTTGCGGAAGTGGAGCGCCACACGCCCGTGCTTCGCGCCCAAACCGGCC
  TCACCCGGCGCGGCCATCCGGCTAGGGAGGGAAATGACCACCTCGGCCTCGCCGGGGTTC
  TGCGGACAAACGTGGGGAGAACTGCGCTGGGGCTGCCGTTCAGTCCCCGCGCCACCTCCA
  CTAGCACAGTGGTAACCCCCATATCCTGCCCCTAAGCCAAAATTCCCGGGGGCACGGGGT
  GTGGGCGGAATTTTGTGAATCTGGGCTTTATCGAGCCGTTCCCTGTCCATGTCCACAGAA
  GGAGATTCACTATTCCAGTGAAGGCACTCGGTCCTCTTAGAAGCTGCATGGTATGTCTGG
  ATTTTCTGTCCAGACTCAGTAGTTCCTTTACGTTGTTTTCGTCGGACATGTGGGCAGACG
  CTGGTGCCCGAGTTCAGATCCTTGGGAAGACAGTAGATCAGACAGGAAGAACGTGGGCCT
  CCGTTGGGCAAATGAAGCTCTTCGCCTTAGGAAATCGTGACAGGAGGCCACAGAGGTGAT
  CTCTACAGTCTCTGGGAGCTGGGAAGTCCAAGGCCTCTTAGTGGGAAAGTGAGGCAGGGT
  CTTGCAGAGCCCACTCTACACACGGCCAGATACAAATGGCTGTAGAGGTCTTAGATAACA
  CCTTCTCGGTCATTTATTTGCACCTCAGAAGAAGCCAGGGCTTCGGTTTTCTGTTTGTTT
  CATCACAGTTGACAGGTTAAAAGCATTCACTGCAGCGATCTATGAGAATTAAGAGGGGAG
  AAGGGGAAATGGAATAAAACTTCGTTTTGTAAAGCTAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001005464 unedited
  CCTACTCGTTTTGTAAACGACTTATATAGGCGGCCGCGATTCCCGGGAATCGTCGACCCA
  CGCGTCCGCGCCGCTGCGCTGGTAAGCCTGTGTTTTGGTTCGCTATGGCCCGTACTAAGC
  AGACTGCTCGCAAGTCGACCGGCGGCAAGGCCCCGAGGAAGCAGCTGGCCACCAAGGCGG
  CCCGCAAGAGCGCGCCGGCCACGGGCGGGGTGAAGAAGCCGCACCGCTACCGGCCCGGCA
  CCGTAGCCCTGCGGGAGATCCGGCGCTACCAGAAGTCCACGGAGCTGCTGATCCGCAAGC
  TGCCCTTCCAGAGGCTGGTACGCGAGATCGCGCAGGACTTTAAGACGGACCTGCGCTTCC
  AGAGCTCGGCCGTGATGGCGCTGCAGGAGGCCAGCGAGGCCTACCTGGTGGGGCTGTTCG
  AAGACACGAACCTGTGCGCCATCCACGCCAAGCGCGTGACCATTATGCCCAAGGACATCC
  AGCTGGCCCGCCGCATCCGTGGAGAGCGGGCTTAAGAAGTGGCGGTTCGAGCGGAGGTTC
  CATCGTATCCAAAAGGCTCTTTTCAGAGCCACCCACATTAGCACTTTGGAAGAAGCTGTA
  CCGATTGCCCTCCGTGCTCCCTCGGCATTAGAGCGGGGAAGGCACTTCCGCTTAGGCTCC
  CCACAAAACGGGGTCGGGTCCCCCTGCTGCAGTGCCGGTCTGAGCCTTGCCGACTTCGGC
  CAATTGTCTATTCCCACCTGCCCGGCTGCGGACCCTTTCTGGGCGCCGGTTCCCGGCTTT
  GGGGGTCCGGTTCCCCCCGCCTAACCGGGCCCTGATTGCGGGTGGGCCCGGGCAGAAAAA
  AGGGGCTCGAGCACAAGCCCGGGTAAAAGGGGAACCTAGGGCCTCGGTCTGAACCCGT
  
• Restriction Sites: Please inquire
• ACCN: NM_001005464
• Insert Size: 1672 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005464.1, NP_001005464.1
• RefSeq Size: 411 bp
• RefSeq ORF: 411 bp
• Locus ID: 333932
• Cytogenetics: 1q21.2
• Protein Pathways: Systemic lupus erythematosus
• Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. [provided by RefSeq, Aug 2015]