CHI3L2 (NM_001025199) Human Untagged Clone

CAT#: SC302363

CHI3L2 (untagged)-Human chitinase 3-like 2 (CHI3L2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHI3L2
• Synonyms: CHIL2; YKL-39; YKL39
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001025199 edited
  GGATCTGCCTACAAACTGGTTTGCTACTTTACCAACTGGTCCCAGGACCGGCAGGAACCA
  GGAAAATTCACCCCTGAGAATATTGACCCCTTCCTATGCTCTCATCTCATCTATTCATTC
  GCCAGCATCGAAAACAACAAGGTTATCATCAAGGACAAGAGTGAAGTGATGCTCTACCAG
  ACCATCAACAGTCTCAAAACCAAGAATCCCAAACTGAAAATTCTCTTGTCCATTGGAGGG
  TACCTGTTTGGTTCCAAAGGGTTCCACCCTATGGTGGATTCTTCTACATCACGCTTGGAA
  TTCATTAACTCCATAATCCTGTTTCTGAGGAACCATAACTTTGATGGACTGGATGTAAGC
  TGGATCTACCCAGATCAGAAAGAAAACACTCATTTCACTGTGCTGATTCATGAGTTAGCA
  GAAGCCTTTCAGAAGGACTTCACAAAATCCACCAAGGAAAGGCTTCTCTTGACTGTGGGC
  GTATCTGCAGGGAGGCAAATGATTGATAACAGCTATCAAGTTGAGAAACTGGCAAAAGAT
  CTGGATTTCATCAACCTCCTGTCCTTTGACTTCCATGGGTCTTGGGAAAAGCCCCTTATC
  ACTGGCCACAACAGCCCTCTGAGCAAGGGGTGGCAGGACAGAGGGCCAAGCTCCTACTAC
  AATGTGGAATATGCTGTGGGGTACTGGATACATAAGGGAATGCCATCAGAGAAGGTGGTC
  ATGGGCATCCCCACATATGGGCACTCCTTCACACTGGCCTCTGCAGAAACCACCGTGGGG
  GCCCCTGCCTCTGGCCCTGGAGCTGCTGGACCCATCACAGAGTCTTCAGGCTTCCTGGCC
  TATTATGAGATCTGCCAGTTCCTGAAAGGAGCCAAGATCACGCGGCTCCAGGATCAGCAG
  GTTCCCTACGCAGTCAAGGGGAACCAGTGGGTGGGCTATGATGATGTGAAGAGTATGGAG
  ACCAAGGTTCAGTTCTTAAAGAATTTAAACCTGGGAGGAGCCATGATCTGGTCTATTGAC
  ATGGATGACTTCACTGGCAAATCCTGCAACCAGGGCCCTTACCCTCTTGTCCAAGCAGTC
  AAGAGAAGCCTTGGCTCCCTGTGAAGGATTAACTTACAGAGAAGCAGGCAAGATGACCTT
  GCTGCCTGGGGCCTGCTCTCTCCCAGGAATTCTCATGTGGGATTCCCCTTGCCAGGCCGG
  CCTTTGGATCTCTCTTCCAAGCCTTTCCTGACTTCCTCTTAGATCATAGATTGGACCTGG
  TTTTGTTTTCCTGCAGCTGTTGACTTGTTGCCCTGAAGTACAATAAAAAAAATTCATTTT
  GCTCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001025199
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a good match to NM_001025199.1 except for one SNP.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025199.1, NP_001020370.1
• RefSeq Size: 1516 bp
• RefSeq ORF: 936 bp
• Locus ID: 1117
• Cytogenetics: 1p13.2
• Gene Summary: 'The protein encoded by this gene is similar to bacterial chitinases but lacks chitinase activity. The encoded protein is secreted and is involved in cartilage biogenesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]'
  Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (c) is shorter at the N-terminus compared to isoform a.