RhoGAP (ARHGAP5) (NM_001030055) Human Untagged Clone

CAT#: SC302503

ARHGAP5 (untagged)-Human Rho GTPase activating protein 5 (ARHGAP5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARHGAP5
• Synonyms: GFI2; p190-B; p190BRhoGAP; RhoGAP5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001030055, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATGGCAAAAAACAAAGAGCCTCGTCCCCCATCCTATACCATCAGTATAGTTGGACTCTCTGGGACTG
  AAAAAGACAAAGGTAACTGTGGAGTTGGAAAGTCTTGTTTGTGCAATAGATTTGTACGCTCAAAAGCAGA
  TGAATATTATCCAGAGCATACTTCTGTGCTTAGCACCATTGACTTTGGAGGACGAGTAGTAAACAATGAT
  CACTTTTTGTACTGGGGTGACATAATACAAAATAGTGAAGATGGAGTAGAATGCAAAATTCATGTCATTG
  AACAAACAGAGTTCATTGATGACCAGACTTTCTTGCCTCATCGGAGTACGAATTTGCAACCATATATAAA
  ACGTGCAGCTGCATCTAAATTGCAGTCAGCAGAAAAACTAATGTACATTTGCACTGATCAGCTAGGCTTA
  GAACAAGACTTTGAACAGAAGCAAATGCCTGAAGGGAAGCTCAACGTAGATGGATTTTTATTATGCATTG
  ATGTAAGTCAAGGATGCAATAGGAAGTTTGATGATCAACTTAAATTTGTGAATAACCTTTTTGTCCAGTT
  ATCAAAATCAAAAAAACCTGTAATAATAGCAGCAACTAAATGTGATGAATGCGTGGATCATTATCTTAGA
  GAAGTTCAGGCATTTGCTTCAAATAAAAAGAACCTTCTTGTAGTGGAAACATCAGCACGATTTAATGTCA
  ACATTGAAACATGTTTTACTGCACTGGTACAAATGTTGGATAAAACTCGTAGCAAGCCTAAAATTATTCC
  CTATTTGGATGCTTATAAAACACAGAGACAACTTGTTGTCACAGCAACAGATAAGTTTGAAAAACTTGTG
  CAGACTGTGAGAGATTATCATGCAACTTGGAAAACTGTTAGTAATAAATTAAAAAATCATCCTGATTATG
  AAGAATACATCAACTTAGAGGGAACAAGAAAGGCCAGAAATACATTCTCAAAACATATAGAACAACTTAA
  ACAGGAACATATAAGAAAAAGGAGAGAAGAGTATATAAATACTTTACCAAGAGCTTTTAACACTCTTTTG
  CCAAATCTAGAAGAGATTGAACATTTGAATTGGTCAGAAGCTTTGAAGTTAATGGAAAAGAGAGCAGATT
  TCCAGTTATGTTTTGTGGTGCTAGAAAAAACTCCTTGGGATGAAACTGACCATATAGACAAAATTAATGA
  TAGGCGGATTCCATTTGACCTCCTGAGCACTTTAGAAGCTGAAAAAGTCTATCAGAACCATGTACAGCAT
  CTGATATCCGAGAAGAGGAGGGTGGAAATGAAGGAAAAATTCAAAAAGACTTTGGAAAAAATTCAATTCA
  TTTCACCAGGGCAGCCATGGGAGGAAGTTATGTGCTTTGTTATGGAGGATGAAGCCTACAAATATATCAC
  TGAGGCTGATAGCAAAGAGGTATATGGTAGGCATCAGCGAGAAATAGTTGAAAAAGCCAAAGAAGAGTTT
  CAAGAAATGCTTTTTGAGCATTCTGAACTTTTTTATGATTTAGATCTTAATGCAACACCTAGTTCAGATA
  AAATGAGTGAAATTCATACAGTTCTGAGTGAAGAACCTAGATATAAAGCTTTACAGAAACTTGCACCTGA
  TAGGGAATCCCTTCTACTTAAGCATATAGGATTTGTTTATCATCCCACTAAAGAAACATGTCTTAGTGGC
  CAAAATTGTACAGACATTAAAGTGGAGCAGTTACTTGCTAGTAGTCTTTTACAGTTGGATCATGGCCGCT
  TAAGATTATATCACGATAGTACCAATATAGATAAAGTTAACCTTTTTATTTTAGGGAAGGATGGCCTTGC
  CCAAGAACTAGCAAATGAGATAAGGACACAATCCACTGATGATGAGTATGCCTTAGATGGAAAAATTTAT
  GAACTTGATCTTCGGCCGGTTGATGCCAAATCGCCTTACTTTTTGAGTCAGTTATGGACTGCCGCCTTTA
  AACCACATGGGTGCTTCTGTGTATTTAATTCCATTGAGTCATTGAGTTTTATTGGGGAATTTATTGGGAA
  AATAAGAACTGAAGCTTCTCAGATCAGAAAAGATAAATACATGGCTAATCTTCCATTTACATTAATTCTG
  GCTAATCAGAGAGATTCCATTAGTAAGAATCTACCAATTCTCAGGCACCAAGGGCAGCAGTTGGCAAACA
  AGTTGCAATGTCCTTTTGTAGATGTACCTGCTGGTACATATCCTCGTAAATTTAATGAAACCCAAATAAA
  GCAAGCTCTCAGAGGAGTATTGGAATCAGTTAAACACAATTTGGATGTGGTGAGCCCAATTCCTGCCAAT
  AAGGACTTATCAGAAGCTGACTTGAGAATTGTCATGTGCGCCATGTGTGGAGATCCATTTAGTGTGGATC
  TTATTCTTTCACCCTTCCTTGATTCTCATTCTTGCAGTGCTGCTCAAGCTGGACAGAATAATTCCCTAAT
  GCTTGATAAAATCATTGGTGAAAAAAGGAGGCGAATACAGATCACAATATTATCATACCACTCTTCAATT
  GGAGTAAGAAAAGATGAACTAGTTCATGGGTATATATTAGTTTACTCTGCAAAACGGAAAGCTTCGATGG
  GAATGCTTCGAGCATTTCTATCAGAAGTTCAAGACACCATTCCTGTACAGCTGGTGGCAGTTACTGACAG
  CCAAGCAGATTTTTTTGAAAATGAGGCTATCAAAGAGTTAATGACTGAAGGAGAACACATTGCAACTGAG
  ATCACTGCTAAATTTACAGCACTGTATTCTTTATCTCAGTATCATCGGCAAACTGAGGTCTTTACTCTGT
  TTTTTAGTGATGTTCTAGAGAAAAAAAATATGATAGAAAATTCTTATTTGTCTGATAATACAAGGGAATC
  AACCCATCAAAGTGAAGATGTTTTTCTACCATCTCCCAGAGACTGTTTTCCCTATAATAACTACCCTGAT
  TCAGATGATGACACAGAAGCACCACCTCCTTATAGTCCAATTGGGGATGATGTACAGTTGCTTCCAACAC
  CTAGTGACCGTTCCAGATATAGATTAGATTTGGAAGGAAATGAGTATCCTATTCATAGTACCCCAAACTG
  TCATGACCATGAACGCAACCATAAAGTGCCTCCACCTATTAAACCTAAACCAGTTGTACCTAAGACAAAT
  GTGAAAAAACTCGATCCAAACCTTTTAAAAACAATTGAAGCTGGTATTGGTAAAAATCCAAGAAAGCAGA
  CTTCCCGGGTGCCTTTGGCACATCCTGAAGATATGGATCCTTCAGATAACTATGCGGAACCCATTGATAC
  AATTTTCAAACAGAAGGGCTATTCTGATGAGATTTATGTTGTCCCAGATGATAGTCAAAATCGTATTAAA
  ATTCGAAACTCATTTGTAAATAACACCCAAGGAGATGAAGAAAATGGGTTTTCTGATAGAACCTCAAAAA
  GTCATGGGGAACGGAGGCCTTCAAAATACAAATATAAATCTAAAACCTTGTTTAGTAAAGCCAAGTCATA
  CTATAGAAGAACACATTCAGATGCCAGTGATGATGAGGCTTTCACCACTTCTAAAACAAAAAGAAAAGGA
  AGACATCGTGGAAGTGAAGAAGATCCACTTCTTTCTCCTGTTGAAACTTGGAAAGGTGGTATTGATAATC
  CTGCAATCACTTCTGACCAGGAGTTAGATGATAAGAAGATGAAGAAGAAAACCCACAAAGTGAAAGAAGA
  TAAAAAGCAGAAAAAGAAAACTAAGAACTTCAATCCACCAACACGTAGAAATTGGGAAAGTAATTACTTT
  GGGATGCCCCTCCAGGATCTGGTTACAGCTGAGAAGCCCATACCACTATTTGTTGAGAAATGTGTGGAAT
  TTATTGAAGATACAGGGTTATGTACCGAAGGACTCTACCGTGTCAGCGGGAATAAAACTGACCAAGACAA
  TATTCAAAAGCAGTTTGATCAAGATCATAATATCAATCTAGTGTCAATGGAAGTAACAGTAAATGCTGTA
  GCTGGAGCCCTTAAAGCTTTCTTTGCAGATCTGCCAGATCCTTTAATTCCATATTCTCTTCATCCAGAAC
  TATTGGAAGCAGCAAAAATCCCGGATAAAACAGAACGTCTTCATGCCTTGAAAGAAATTGTTAAGAAATT
  TCATCCTGTAAACTATGATGTATTCAGATACGTGATAACACATCTAAACAGGGTTAGTCAGCAACATAAA
  ATCAACCTAATGACAGCAGACAACTTATCCATCTGTTTTTGGCCAACCTTGATGAGACCTGATTTTGAAA
  ATCGAGAGTTTCTGTCTACTACTAAGATTCATCAATCTGTTGTTGAAACATTCATTCAGCAGTGTCAGTT
  TTTCTTTTACAATGGAGAAATTGTAGAAACGACAAACATTGTGGCTCCTCCACCACCTTCAAACCCAGGA
  CAGTTGGTGGAACCAATGGTGCCACTTCAGTTGCCGCCACCATTGCAACCTCAGCTGATACAACCACAAT
  TACAAACGGATCCTCTTGGTATTATATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001030055
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001030055.1, NP_001025226.1
• RefSeq Size: 9604 bp
• RefSeq ORF: 4509 bp
• Locus ID: 394
• Cytogenetics: 14q12
• Protein Pathways: Focal adhesion, Leukocyte transendothelial migration
• Gene Summary: 'Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).