BMAL1 (ARNTL) (NM_001030273) Human Untagged Clone

CAT#: SC302508

ARNTL (untagged)-Human aryl hydrocarbon receptor nuclear translocator-like (ARNTL), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARNTL
• Synonyms: bHLHe5; BMAL1; BMAL1c; JAP3; MOP3; PASD3; TIC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001030273 edited
  TCGGGCGCGGGCGCTCCCGGCGAGCCACGGTGGTGCTGGCTAGAGTGTATACGTTTGGAC
  CCAAGCTTAACTTTTCCAATGTGGAATCCTGGGCCTTCATTGGTTTTTGGAAGAAGCTGA
  CCGCCTGAAAAGAAATTATAAAACATGAAAATCGCTTTGAGGTGACCAAGTCCAGAGGCC
  CCTAACTCCTCCCAAGCTGGATCTGGGGTGTAAGAACTGTGACTTCAGATCATCCAATGG
  CAGACCAGAGAATGGACATTTCTTCAACCATCAGTGATTTCATGTCCCCGGGCCCCACCG
  ACCTGCTTTCCAGCTCTCTTGGTACCAGTGGTGTGGATTGCAACCGCAAACGGAAAGGCA
  GCTCCACTGACTACCAGTCGAATTTGGGGAGCACAATGGCTGGAGGTCAGATGCCCACTA
  GGAGATGCTATGATTAATATAGAAAGCATGGACACAGACAAAGATGACCCTCATGGAAGG
  TTAGAATATACAGAACACCAAGGAAGGATAAAAAATGCAAGGGAAGCTCACAGTCAGATT
  GAAAAGCGGCGTCGGGATAAAATGAACAGTTTTATAGATGAATTGGCTTCTTTGGTACCA
  ACATGCAACGCAATGTCCAGGAAATTAGATAAACTTACTGTGCTAAGGATGGCT:GTTCA
  GCACATGAAAACATTAAGAGGTGCCACCAATCCATACACAGAAGCAAACTACAAACCAAC
  TTTTCTATCAGACGATGAATTGAAACACCTCATTCTCAGGGCAGCAGATGGATTTTTGTT
  TGTCGTAGGATGTGACCGAGGGAAGATACTCTTTGTCTCAGAGTCTGTCTTCAAGATCCT
  CAACTACAGCCAGAATGATCTGATTGGTCAGAGTTTGTTTGACTACCTGCATCCTAAAGA
  TATTGCCAAAGTCAAGGAGCAGCTCTCCTCCTCTGACACCGCACCCCGGGAGCGGCTCAT
  AGATGCAAAAACTGGACTTCCAGTTAAAACAGATATAACCCCTGGGCCATCTCGATTATG
  TTCTGGAGCACGACGTTCTTTCTTCTGTAGGATGAAGTGTAACAGGCCTTCAGTAAAGGT
  TGAAGACAAGGACTTCCCCTCTACCTGCTCAAAGAAAAAAGCAGATCGAAAAAGCTTCTG
  CACAATCCACAGCACAGGCTATTTGAAAAGCTGGCCACCCACAAAGATGGGGCTGGATGA
  AGACAACGAACCAGACAATGAGGGGTGTAACCTCAGCTGCCTCGTCGCAATTGGACGACT
  GCATTCTCATGTAGTTCCACAACCAGTGAACGGGGAAATCAGGGTGAAATCTATGGAATA
  TGTTTCTCGGCACGCGATAGATGGAAAGTTTGTTTTTGTAGACCAGAGGGCAACAGCTAT
  TTTGGCATATTTACCACAAGAACTTCTAGGCACATCGTGTTATGAATATTTTCACCAAGA
  TGACATAGGACATCTTGCAGAATGTCATAGGCAAGTTTTACAGACGAGAGAAAAAATTAC
  AACTAATTGCTATAAATTTAAAATCAAAGATGGTTCTTTTATCACACTACGGAGTCGATG
  GTTCAGTTTCATGAACCCTTGGACCAAGGAAGTAGAATATATTGTCTCAACTAACACTGT
  TGTTTTAGCCAACGTCCTGGAAGGCGGGGACCCAACCTTCCCACAGCTCACAGCATCCCC
  CCACAGCATGGACAGCATGCTGCCCTCTGGAGAAGGTGGCCCAAAGAGGACCCACCCCAC
  TGTTCCAGGGATTCCAGGGGGAACCCGGGCTGGGGCAGGAAAAATAGGCCGAATGATTGC
  TGAGGAAATCATGGAAATCCACAGGATAAGAGGGTCATCGCCTTCTAGCTGTGGCTCCAG
  CCCATTGAACATCACGAGTACGCCTCCCCCTGATGCCTCTTCTCCAGGAGGCAAGAAGAT
  TTTAAATGGAGGGACTCCAGACATTCCTTCCAGTGGCCTACTATCAGGCCAGGCTCAGGA
  GAACCCAGGTTATCCATATTCTGATAGTTCTTCTATTCTTGGTGAGAACCCCCACATAGG
  TATAGACATGATTGACAACGACCAAGGATCAAGTAGTCCCAGTAATGATGAGGCAGCAAT
  GGCTGTCATCATGAGCCTCTTGGAAGCAGATGCTGGACTGGGTGGCCCTGTTGACTTTAG
  TGACTTGCCATGGCCGCTGTAAACACTACATGTTGCTTTGGCAACAGCTATAGTATCAAA
  GTGCATTACTGGTGGAGTTTTACAGTCTGTGAAGCTTACTGGATAAGGAGAGAATAGCTT
  TTATGTACTGACTTCATAAAAGCCATCTCAGAGCCATTGATACAAGTCAATCTTACTATA
  TGTAACTTCAGACAAAGTGGAACTAAGCCTGCTCCAGTGTTTCCTCATCATTGATTATTG
  GGCTAGCTGTGGATAGCTTGCATTAATTGTATATTTTGGATTCTGTTTGTGTTGAATTTT
  TTAATCATTGTGCACAGAAGCATCATTGGTAGCTTTTATATGCAAATGGTCATTTCAGAT
  GTATGGTGTTTTTACACTACAAAGAAGTCCCCCATGTGGATATTTCTTATACTAATTGTA
  TCATAAAGCCGTTTATTCTTCCTTGTAAGAATCCTTTACTATAAATATGGGTTAAAGTAT
  AATGTACTAGACAGTTAAATATTTTTAATAAATGTTTCCCTTGTTCTATAAAAAAAAAAA
  AAAAAAAAAAAATAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001030273
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001030273.1, NP_001025444.1
• RefSeq Size: 2828 bp
• RefSeq ORF: 1749 bp
• Locus ID: 406
• Cytogenetics: 11p15.3
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• Gene Summary: 'The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]'
  Transcript Variant: This variant (3) differs in the 5' UTR, contains an alternate exon, and uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 4. The resulting isoform (b) has a shorter and distinct N-terminus and lacks an alternate internal aa compared to isoform c. Variants 3 and 22 both encode isoform b.