BMAL1 (ARNTL) (NM_001030273) Human Untagged Clone

CAT#: SC302508

ARNTL (untagged)-Human aryl hydrocarbon receptor nuclear translocator-like (ARNTL), transcript variant 3


  "NM_001030273" in other vectors (6)

Reconstitution Protocol

USD 760.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ARNTL
Synonyms bHLHe5; BMAL1; BMAL1c; JAP3; MOP3; PASD3; TIC
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_001030273 edited
TCGGGCGCGGGCGCTCCCGGCGAGCCACGGTGGTGCTGGCTAGAGTGTATACGTTTGGAC
CCAAGCTTAACTTTTCCAATGTGGAATCCTGGGCCTTCATTGGTTTTTGGAAGAAGCTGA
CCGCCTGAAAAGAAATTATAAAACATGAAAATCGCTTTGAGGTGACCAAGTCCAGAGGCC
CCTAACTCCTCCCAAGCTGGATCTGGGGTGTAAGAACTGTGACTTCAGATCATCCAATGG
CAGACCAGAGAATGGACATTTCTTCAACCATCAGTGATTTCATGTCCCCGGGCCCCACCG
ACCTGCTTTCCAGCTCTCTTGGTACCAGTGGTGTGGATTGCAACCGCAAACGGAAAGGCA
GCTCCACTGACTACCAGTCGAATTTGGGGAGCACAATGGCTGGAGGTCAGATGCCCACTA
GGAGATGCTATGATTAATATAGAAAGCATGGACACAGACAAAGATGACCCTCATGGAAGG
TTAGAATATACAGAACACCAAGGAAGGATAAAAAATGCAAGGGAAGCTCACAGTCAGATT
GAAAAGCGGCGTCGGGATAAAATGAACAGTTTTATAGATGAATTGGCTTCTTTGGTACCA
ACATGCAACGCAATGTCCAGGAAATTAGATAAACTTACTGTGCTAAGGATGGCT:GTTCA
GCACATGAAAACATTAAGAGGTGCCACCAATCCATACACAGAAGCAAACTACAAACCAAC
TTTTCTATCAGACGATGAATTGAAACACCTCATTCTCAGGGCAGCAGATGGATTTTTGTT
TGTCGTAGGATGTGACCGAGGGAAGATACTCTTTGTCTCAGAGTCTGTCTTCAAGATCCT
CAACTACAGCCAGAATGATCTGATTGGTCAGAGTTTGTTTGACTACCTGCATCCTAAAGA
TATTGCCAAAGTCAAGGAGCAGCTCTCCTCCTCTGACACCGCACCCCGGGAGCGGCTCAT
AGATGCAAAAACTGGACTTCCAGTTAAAACAGATATAACCCCTGGGCCATCTCGATTATG
TTCTGGAGCACGACGTTCTTTCTTCTGTAGGATGAAGTGTAACAGGCCTTCAGTAAAGGT
TGAAGACAAGGACTTCCCCTCTACCTGCTCAAAGAAAAAAGCAGATCGAAAAAGCTTCTG
CACAATCCACAGCACAGGCTATTTGAAAAGCTGGCCACCCACAAAGATGGGGCTGGATGA
AGACAACGAACCAGACAATGAGGGGTGTAACCTCAGCTGCCTCGTCGCAATTGGACGACT
GCATTCTCATGTAGTTCCACAACCAGTGAACGGGGAAATCAGGGTGAAATCTATGGAATA
TGTTTCTCGGCACGCGATAGATGGAAAGTTTGTTTTTGTAGACCAGAGGGCAACAGCTAT
TTTGGCATATTTACCACAAGAACTTCTAGGCACATCGTGTTATGAATATTTTCACCAAGA
TGACATAGGACATCTTGCAGAATGTCATAGGCAAGTTTTACAGACGAGAGAAAAAATTAC
AACTAATTGCTATAAATTTAAAATCAAAGATGGTTCTTTTATCACACTACGGAGTCGATG
GTTCAGTTTCATGAACCCTTGGACCAAGGAAGTAGAATATATTGTCTCAACTAACACTGT
TGTTTTAGCCAACGTCCTGGAAGGCGGGGACCCAACCTTCCCACAGCTCACAGCATCCCC
CCACAGCATGGACAGCATGCTGCCCTCTGGAGAAGGTGGCCCAAAGAGGACCCACCCCAC
TGTTCCAGGGATTCCAGGGGGAACCCGGGCTGGGGCAGGAAAAATAGGCCGAATGATTGC
TGAGGAAATCATGGAAATCCACAGGATAAGAGGGTCATCGCCTTCTAGCTGTGGCTCCAG
CCCATTGAACATCACGAGTACGCCTCCCCCTGATGCCTCTTCTCCAGGAGGCAAGAAGAT
TTTAAATGGAGGGACTCCAGACATTCCTTCCAGTGGCCTACTATCAGGCCAGGCTCAGGA
GAACCCAGGTTATCCATATTCTGATAGTTCTTCTATTCTTGGTGAGAACCCCCACATAGG
TATAGACATGATTGACAACGACCAAGGATCAAGTAGTCCCAGTAATGATGAGGCAGCAAT
GGCTGTCATCATGAGCCTCTTGGAAGCAGATGCTGGACTGGGTGGCCCTGTTGACTTTAG
TGACTTGCCATGGCCGCTGTAAACACTACATGTTGCTTTGGCAACAGCTATAGTATCAAA
GTGCATTACTGGTGGAGTTTTACAGTCTGTGAAGCTTACTGGATAAGGAGAGAATAGCTT
TTATGTACTGACTTCATAAAAGCCATCTCAGAGCCATTGATACAAGTCAATCTTACTATA
TGTAACTTCAGACAAAGTGGAACTAAGCCTGCTCCAGTGTTTCCTCATCATTGATTATTG
GGCTAGCTGTGGATAGCTTGCATTAATTGTATATTTTGGATTCTGTTTGTGTTGAATTTT
TTAATCATTGTGCACAGAAGCATCATTGGTAGCTTTTATATGCAAATGGTCATTTCAGAT
GTATGGTGTTTTTACACTACAAAGAAGTCCCCCATGTGGATATTTCTTATACTAATTGTA
TCATAAAGCCGTTTATTCTTCCTTGTAAGAATCCTTTACTATAAATATGGGTTAAAGTAT
AATGTACTAGACAGTTAAATATTTTTAATAAATGTTTCCCTTGTTCTATAAAAAAAAAAA
AAAAAAAAAAAATAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_001030273
Insert Size 2700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001030273.1, NP_001025444.1
RefSeq Size 2828 bp
RefSeq ORF 1749 bp
Locus ID 406
Cytogenetics 11p15.3
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Circadian rhythm - mammal
Gene Summary 'The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]'
Transcript Variant: This variant (3) differs in the 5' UTR, contains an alternate exon, and uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 4. The resulting isoform (b) has a shorter and distinct N-terminus and lacks an alternate internal aa compared to isoform c. Variants 3 and 22 both encode isoform b.

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