PRPF40B (NM_001031698) Human Untagged Clone

CAT#: SC302543

PRPF40B (untagged)-Human PRP40 pre-mRNA processing factor 40 homolog B (S. cerevisiae) (PRPF40B), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRPF40B
• Synonyms: HYPC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001031698, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCGGTTCCCGATTCTGGTCCCCGGCCCCCAGCAGCGCCTGCCCCCTTCCCACCGGGGCCCCCCATGA
  TGCCACCACCCTTCATGCCCCCTCCAGGGATCCCCCCACCCTTTCCTCCGATGGGGCTACCCCCCATGAG
  TCAGAGACCACCAGCTATCCCCCCCATGCCACCTGGCATCCTGCCCCCAATGCTTCCACCAATGGGGGCG
  CCACCACCACTCACACAGATACCAGGAATGGTACCTCCGATGATGCCAGGAATGCTGATGCCAGCGGTGC
  CTGTCACCGCAGCGACGGCTCCGGGTGCGGACACCGCCAGCTCTGCTGTGGCTGGGACAGGCCCTCCGAG
  GGCCCTATGGAGTGAGCATGTGGCCCCAGATGGGCGCATCTACTACTACAATGCTGACGACAAGCAGTCC
  GTGTGGGAGAAGCCCAGCGTGCTCAAGTCCAAGGCAGAGCTGCTCCTGTCCCAATGTCCCTGGAAAGAGT
  ACAAGTCGGACACAGGCAAACCTTATTACTATAACAACCAGAGTAAAGAGTCCCGCTGGACCCGGCCCAA
  GGATCTGGATGACCTAGAGGTTCTAGTCAAACAAGAGGCTGCAGGGAAACAGCAGCAGCAGCTGCCACAG
  ACACTTCAGCCACAGCCACCTCAGCCACAGCCTGACCCCCCACCTGTGCCTCCTGGCCCCACCCCAGTGC
  CCACAGGCCTCCTGGAACCTGAGCCAGGTGGGAGTGAAGATTGTGATGTGTTGGAGGCCACCCAGCCCCT
  GGAACAGGGGTTCCTGCAGCAGCTGGAGGAGGGCCCCAGCAGTTCTGGACAGCATCAGCCACAGCAGGAG
  GAGGAGGAATCAAAGCCAGAACCAGAGAGGTCTGGCCTCAGTTGGAGCAACCGGGAGAAGGCAAAGCAGG
  CATTCAAGGAACTGCTGAGGGACAAGGCTGTCCCCTCCAATGCCTCATGGGAACAGGCCATGAAGATGGT
  GGTCACCGACCCCCGTTACAGTGCCTTGCCTAAACTGAGTGAGAAAAAGCAGGCATTCAATGCCTACAAG
  GCGCAGCGGGAGAAGGAGGAGAAGGAGGAGGCCCGGCTAAGGGCCAAAGAGGCCAAGCAGACCCTGCAGC
  ATTTCCTGGAGCAGCATGAACGCATGACCTCCACCACCCGCTACCGGCGGGCAGAACAGACCTTTGGGGA
  GCTGGAGGTCTGGGCTGTGGTCCCTGAGAGGGATCGAAAAGAGGTTTATGATGATGTCCTCTTCTTCCTG
  GCCAAGAAGGAGAAGGAACAGGCCAAGCAGCTCCGGCGCCGCAATATCCAGGCCCTAAAGAGCATCCTGG
  ATGGGATGAGTAGTGTCAACTTCCAAACCACGTGGTCCCAGGCCCAGCAGTACCTCATGGATAACCCCAG
  CTTTGCTCAGGACCATCAGCTGCAGAACATGGACAAGGAAGATGCACTGATCTGTTTTGAGGAGCACATC
  CGAGCTTTGGAGAGGGAAGAGGAGGAGGAACGGGAGCGGGCCCGGCTTCGGGAGCGACGCCAACAACGCA
  AGAATCGGGAGGCCTTCCAGACCTTCCTGGACGAGCTGCATGAGACAGGGCAGCTGCACTCTATGTCCAC
  CTGGATGGAGCTATATCCAGCAGTCAGCACTGATGTCCGCTTTGCCAACATGCTGGGCCAGCCGGGCTCC
  ACCCCTCTGGACTTATTCAAGTTCTATGTGGAGGAGTTGAAGGCACGATTCCATGATGAAAAGAAGATCA
  TTAAGGACATCCTTAAGGACCGGGGCTTCTGCGTGGAGGTGAACACGGCCTTTGAGGACTTCGCCCACGT
  CATAAGCTTTGACAAGAGGGCTGCCGCACTGGACGCAGGCAACATCAAGCTGACCTTCAATAGTCTGCTG
  GAGAAAGCAGAGGCACGGGAGAGGGAGCGGGAGAAGGAGGAGGCACGCAGGATGCGGCGCAGGGAAGCTG
  CCTTTCGAAGCATGCTGAGGCAGGCTGTGCCTGCTCTGGAGCTAGGCACTGCCTGGGAAGAGGTCCGTGA
  GCGTTTTGTGTGTGACTCAGCCTTTGAGCAGATCACCCTGGAGTCGGAGCGGATCCGGCTCTTCCGGGAG
  TTCCTACAGGTGCTGGAGACTGAATGCCAGCACCTCCACACCAAAGGCCGAAAGCATGGCAGGAAAGGCA
  AGAAGCACCATCACAAGCGTTCCCACTCACCCTCAGGCTCTGAGTCAGAAGAAGAGGAGCTGCCCCCACC
  ATCTCTCCGGCCCCCCAAGCGGAGGAGGCGGAACCCCTCAGAGTCAGGCTCTGAGCCCTCTTCCTCACTT
  GATTCAGTTGAAAGTGGGGGTGCTGCCCTTGGAGGACGGGGCTCCCCTTCCTCCCATCTTCTTGGAGCAG
  ATCATGGCCTTCGGAAAGCCAAGAAACCAAAAAAGAAAACTAAGAAGAGAAGACACAAGTCGAATAGTCC
  TGAGAGTGAGACAGACCCTGAGGAGAAAGCTGGCAAGGAGAGCGATGAGAAAGAACAAGAACAGGACAAG
  GACAGGGAGCTCCAACAGGCAGAGCTCCCTAACCGTTCCCCAGGCTTTGGAATCAAGAAGGAGAAGACAG
  GCTGGGACACGTCAGAAAGTGAGCTGAGTGAGGGTGAGCTGGAGAGGCGGCGGCGGACACTCCTACAGCA
  GCTGGATGATCACCAGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001031698
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031698.2, NP_001026868.2
• RefSeq Size: 3350 bp
• RefSeq ORF: 2679 bp
• Locus ID: 25766
• Cytogenetics: 12q13.12
• Protein Pathways: Spliceosome
• Gene Summary: This gene encodes a WW-domain containing protein similar to yeast splicing factor PRP40. This protein has been shown to interact with Huntingtin and methyl CpG binding protein 2 (MeCP2). Alternative splicing results in different transcript variants. [provided by RefSeq, Aug 2014]
  Transcript Variant: This variant (1) encodes the longer isoform (1).